Evans blue dye-enhanced imaging of the brain microvessels using spectral focusing coherent anti-Stokes Raman scattering microscopy

Lee, Boram; Joo, K.; Choi, Eunsuk; Jahng, Junghoon; Kim, Hyunmin; Kim, Eun-Joo

doi:10.1371/journal.pone.0185519

Cited by 6 publications

(1 citation statement)

References 32 publications

(36 reference statements)

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“…Coherent anti-Stokes Raman scattering (CARS) microscopy is another non-invasive advanced imaging technology that has been widely used in biochemistry, pharmacology, neurobiology, and other fields, as this label-free imaging method is advantageous for the imaging of biologically important macromolecules [ 12 , 13 , 14 , 15 ] such as proteins, lipids, nucleic acids, and carbohydrates [ 16 , 17 , 18 , 19 , 20 ]. Since blood vessels are mainly composed of lipid- and protein-rich endothelial cells, we envisage that CARS can also be potentially implemented to acquire the label-free long-term imaging of blood vessels [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Long-Term Repeatable In Vivo Monitoring of Amyloid-β Plaques and Vessels in Alzheimer’s Disease Mouse Model with Combined TPEF/CARS Microscopy

Luo

Samanta

et al. 2022

Biomedicines

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Long-term, repeatable monitoring of the appearance and progress of Alzheimer’s disease (AD) in real time can be extremely beneficial to acquire highly reliable diagnostic insights, which is crucial for devising apt strategies towards effective AD treatment. Herein, we present an optimized innovative cranial window imaging method for the long-term repeatable imaging of amyloid-β (Aβ) plaques and vessels in an AD mouse model. Basically, two-photon excitation fluorescence (TPEF) microscopy was used to monitor the fluorescently labeled Aβ plaques, whereas the label-free blood vessels were studied using coherent anti-Stokes Raman scattering (CARS) microscopy in the live in vivo AD mouse model. It was possible to clearly observe the Aβ deposition and vascular structure in the target cortex localization for 31 weeks in the AD mouse model using this method. The combined TPEF/CARS imaging studies were also instrumental in realizing the relationship between the tendency of Aβ deposition and ageing. Essentially, the progression of cerebral amyloid angiopathy (CAA) in the AD mouse model was quantitatively characterized, which revealed that the proportion Aβ deposition in the unit vessel can increase from 13.63% to 28.80% upon increasing the age of mice from 8 months old to 14 months old. The proposed imaging method provided an efficient, safe, repeatable platform with simple target localization aptitude towards monitoring the brain tissues, which is an integral part of studying any brain-related physiological or disease conditions to extract crucial structural and functional information.

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Section: Introductionmentioning

confidence: 99%

Long-Term Repeatable In Vivo Monitoring of Amyloid-β Plaques and Vessels in Alzheimer’s Disease Mouse Model with Combined TPEF/CARS Microscopy

Luo

Samanta

et al. 2022

Biomedicines

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Raman spectroscopy and neuroscience: from fundamental understanding to disease diagnostics and imaging

Payne

Moody

Wood

et al. 2020

Analyst

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Adenovirus-delivered GFP-HO-1C[INCREMENT]23 attenuates blood–spinal cord barrier permeability after rat spinal cord contusion

Chang

Bi²,

Meng³

et al. 2018

NeuroReport

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The blood-spinal cord barrier (BSCB) plays a key role in maintaining the microenvironment and is primarily composed of tight junction proteins and nonfenestrated capillary endothelial cells. After injury, BSCB damage results in increasing capillary permeability and release of inflammatory factors. Recent studies have reported that haem oxygenase-1 (HO-1) fragments lacking 23 amino acids at the C-terminus (HO-1C[INCREMENT]23) exert novel anti-inflammatory and antioxidative effects in vitro. However, no study has identified the role of HO-1C[INCREMENT]23 in vivo. We aimed to investigate the protective effects of HO-1C[INCREMENT]23 on the BSCB after spinal cord injury (SCI) in a rat model. Here, adenoviral HO-1C[INCREMENT]23 (Ad-GFP-HO-1C[INCREMENT]23) was intrathecally injected into the 10th thoracic spinal cord segment (T10) 7 days before SCI. In addition, nuclear and cytoplasmic extraction and immunofluorescence staining of HO-1 were used to examine the effect of Ad-GFP-HO-1C[INCREMENT]23 on HO-1 nuclear translocation. Evan's blue staining served as an index of capillary permeability and was detected by fluorescence microscopy at 633 nm. Western blotting was also performed to detect tight junction protein expression. The Basso, Beattie and Bresnahan score was used to evaluate kinematic functional recovery through the 28th day after SCI. In this study, the Ad-GFP-HO-1C[INCREMENT]23 group showed better kinematic functional recovery after SCI than the Ad-GFP and Vehicle groups, as well as smaller reductions in TJ proteins and capillary permeability compared with those in the Ad-GFP and Vehicle groups. These findings indicated that Ad-GFP-HO-1C[INCREMENT]23 might have a potential therapeutic effect that is mediated by its protection of BSCB integrity.

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Evans blue dye-enhanced imaging of the brain microvessels using spectral focusing coherent anti-Stokes Raman scattering microscopy

Cited by 6 publications

References 32 publications

Long-Term Repeatable In Vivo Monitoring of Amyloid-β Plaques and Vessels in Alzheimer’s Disease Mouse Model with Combined TPEF/CARS Microscopy

Long-Term Repeatable In Vivo Monitoring of Amyloid-β Plaques and Vessels in Alzheimer’s Disease Mouse Model with Combined TPEF/CARS Microscopy

Raman spectroscopy and neuroscience: from fundamental understanding to disease diagnostics and imaging

Adenovirus-delivered GFP-HO-1C[INCREMENT]23 attenuates blood–spinal cord barrier permeability after rat spinal cord contusion

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