Evaluations of the mutagenicity of a pigment extract from bulb culture of Hippeastrum reticulatum

Nitteranon, Viriya; Kittiwongwattana, Chokchai; Vuttipongchaikij, Supachai; Sakulkoo, Jenjira; Srijakkoat, Monthira; Chokratin, Pakawieng; Harinasut, Poontariga; Suputtitada, Saowanee; Apisitwanich, Somsak

doi:10.1016/j.fct.2014.04.007

Cited by 5 publications

(4 citation statements)

References 33 publications

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“…Physiological activities reported for plants of this genus include psychopharmacological [ 20 ], against Trichomonas vaginalis [ 21 ] and cytotoxicity [ 22 , 23 ]. The species H. reticulatum is widely used as an ornamental plant due to its colorful flowers ranging from light pink to dark purple [ 24 ]. In recent years, some studies have been published about its primary metabolites, pigment constituents (anthocyanins), botanical characterization, karyotypes and phylogenesis [ 9 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…The species H. reticulatum is widely used as an ornamental plant due to its colorful flowers ranging from light pink to dark purple [ 24 ]. In recent years, some studies have been published about its primary metabolites, pigment constituents (anthocyanins), botanical characterization, karyotypes and phylogenesis [ 9 , 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

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Hippeastrum reticulatum (Amaryllidaceae): Alkaloid Profiling, Biological Activities and Molecular Docking

et al. 2017

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The Amaryllidaceae family has proven to be a rich source of active compounds, which are characterized by unique skeleton arrangements and a broad spectrum of biological activities. The aim of this work was to perform the first detailed study of the alkaloid constituents of Hippeastrum reticulatum (Amaryllidaceae) and to determine the anti-parasitological and cholinesterase (AChE and BuChE) inhibitory activities of the epimers (6α-hydroxymaritidine and 6β-hydroxymaritidine). Twelve alkaloids were identified in H. reticulatum: eight known alkaloids by GC-MS and four unknown (6α-hydroxymaritidine, 6β-hydroxymaritidine, reticulinine and isoreticulinine) by NMR. The epimer mixture (6α-hydroxymaritidine and 6β-hydroxymaritidine) showed low activity against all protozoan parasites tested and weak AChE-inhibitory activity. Finally, a molecular docking analysis of AChE and BuChE proteins showed that isoreticulinine may be classified as a potential inhibitory molecule since it can be stabilized in the active site through hydrogen bonds, π-π stacking and hydrophobic interactions.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hippeastrum reticulatum (Amaryllidaceae): Alkaloid Profiling, Biological Activities and Molecular Docking

et al. 2017

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“…Nonetheless, regarding the toxicity of anthocyanins, previous studies have indicated that this compound is widely used as a natural colorant in food and beverages in Europe, Japan, and the United States [ 22 ]. Additionally, JECFA concluded that anthocyanin-containing extracts had a very low or negligible level of toxicity upon consumption [ 24 , 54 ].…”

Section: Resultsmentioning

confidence: 99%

Production and Analytical Aspects of Natural Pigments to Enhance Alternative Meat Product Color

Bakhsh

Cho

Baritugo

et al. 2023

Foods

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Color is a major feature that strongly influences the consumer’s perception, selection, and acceptance of various foods. An improved understanding regarding bio-safety protocols, health welfare, and the nutritional importance of food colorants has shifted the attention of the scientific community toward natural pigments to replace their toxic synthetic counterparts. However, owing to safety and toxicity concerns, incorporating natural colorants directly from viable sources into plant-based meat (PBM) has many limitations. Nonetheless, over time, safe and cheap extraction techniques have been developed to extract the purified form of coloring agents from raw materials to be incorporated into PBM products. Subsequently, extracted anthocyanin has displayed compounds like Delphinidin-3-mono glucoside (D3G) at 3.1 min and Petunidin-3-mono glucoside (P3G) at 5.1 277, 515, and 546 nm at chromatographic lambda. Fe-pheophytin was successfully generated from chlorophyll through the ion exchange method. Likewise, the optical density (OD) of synthesized leghemoglobin (LegH) indicated that pBHA bacteria grow more rigorously containing ampicillin with a dilution factor of 10 after 1 h of inoculation. The potential LegH sequence was identified at 2500 bp through gel electrophoresis. The color coordinates and absorbance level of natural pigments showed significant differences (p < 0.05) with the control. The development of coloring agents originating from natural sources for PBM can be considered advantageous compared to animal myoglobin in terms of health and functionality. Therefore, the purpose of this study was to produce natural coloring agents for PBM by extracting and developing chlorophyll from spinach, extracting anthocyanins from black beans, and inserting recombinant plasmids into microorganisms to produce LegH.

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“…However, a study on in vivo micronucleated reticulocyte assay revealed that no mutagenicity was observed up to 1 g/kg bw of anthocyanin-based pigment extract [31].…”

Section: Micronucleus Assaymentioning

confidence: 99%

Assessment of Potential Mutagenic Effect of Colorant of Some Commercial Fruit Drinks in Mice

Hassanane¹,

Girgis²,

Shoman³

2014

J Nutr Food Sci

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Due to the large consumption of commercial fruit drinks worldwide in recent years and considering that some of the components present in their composition cause potential risks to human health, therefore, this study was designed to evaluate the potential mutagenic effect of colorants of commercial fruit drinks (pear, cherry, strawberries and red grape) stored at 4°C for six months, on mice using comet as say, DNA fragmentation , and micronucleus test as a good indicators for strand breaks in DNA, as well measuring the malondialdehyde (MDA) level. Three doses 0.8, 1.6 and 2.4 mg/kg bw of 4 commercial fruit drinks were administered orally for mice for 3 weeks beside the control group. Mice were sacrificed 24 hours after the last dose and subjected to micronucleus and comet assays as well DNA degradation and MDA analysis. The results revealed that a significant increase in tail length of comet percentages from blood cells as well in the fre quency of micronucleated cells (MNCs) and DNA fragmentation following administration of commercial fruit drinks was achieved compared to control group. The level of MDA was increased (P<0.05) significantly after administration of commercial fruit drinks especially with the high dose (2.4 mg/ kg bw) of treatment compared to control. In conclusion, this study serves as a warning about the consumption of commercial fruit drinks and for the need for further studies in order to evaluate the long term mutagenic effect of these colorants on human health since some soft drinks are consumed daily by a significant proportion of the world population.Citation: Hassanane MM, Girgis SM, Shoman TMT (2014) Assessment of Potential Mutagenic Effect of Colorant of Some Commercial Fruit Drinks in Mice.

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Evaluations of the mutagenicity of a pigment extract from bulb culture of Hippeastrum reticulatum

Cited by 5 publications

References 33 publications

Hippeastrum reticulatum (Amaryllidaceae): Alkaloid Profiling, Biological Activities and Molecular Docking

Hippeastrum reticulatum (Amaryllidaceae): Alkaloid Profiling, Biological Activities and Molecular Docking

Production and Analytical Aspects of Natural Pigments to Enhance Alternative Meat Product Color

Assessment of Potential Mutagenic Effect of Colorant of Some Commercial Fruit Drinks in Mice

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