Evaluations of Shorter Exposures of Contact Lens Cleaning Solutions against Fusarium oxysporum Species Complex and Fusarium solani Species Complex To Simulate Inappropriate Usage

Ramani, Rama; Chaturvedi, Vishnu

doi:10.1128/aac.00634-09

Cited by 6 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…22,30 In fact, most contact lens cases develop biofilm with use. Another limitation is that we tested only one isolate of Curvularia sp.…”

Section: Discussionmentioning

confidence: 99%

“…7,22,[26][27][28][29][30][31][32][33] In our study, we investigated the susceptibility of 13 filamentous fungal strains isolated from human corneal ulcers to 6 contact lens disinfecting solutions. On the whole, 3% hydrogen peroxidebased Arion Cronos and Oxysept Comfort demonstrated a good fungicidal activity; indeed, they were able to kill most of the fungal isolates within the minimum recommended disinfection time (1 and 6 h, respectively).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

In Vitro Efficacy of Contact Lens Solutions Against Various Corneal Fungal Isolates

Pinna

Usai²,

Zanetti³

et al. 2013

Journal of Ocular Pharmacology and Therapeutics

View full text Add to dashboard Cite

show abstract

“…22,30 In fact, most contact lens cases develop biofilm with use. Another limitation is that we tested only one isolate of Curvularia sp.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In Vitro Efficacy of Contact Lens Solutions Against Various Corneal Fungal Isolates

Pinna

Usai²,

Zanetti³

et al. 2013

Journal of Ocular Pharmacology and Therapeutics

View full text Add to dashboard Cite

show abstract

“…Relatively little attention has been paid to the efficacy of disinfecting solutions against fungi [19,[37][38][39][40][41][42][43]. In the U.S., only 1-log reduction of fungal organisms within the recommended disinfection time is required by the FDA to meet the primary Stand Alone Test criteria for contact lens disinfection.…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of various corneal fungal isolates and Pseudomonas aeruginosa to contact lens disinfecting solutions

Pinna

Usai

Zanetti

et al. 2013

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: We aimed to investigate the susceptibility of a combined inoculum of Pseudomonas aeruginosa and different fungal strains to 6 soft contact lens disinfectants. Methodology: One corneal isolate of P. aeruginosa and 13 corneal fungal isolates (9 Aspergillus spp, 3 Fusarium spp, 1 Curvularia sp.) were used. The following solutions were tested: Arion Cronos, Complete RevitaLens, Dua Elite, Opti-Free Express, Regard, Oxysept Comfort, and Oxysept Comfort without catalase. The effect of the solutions was assessed on a combined inoculum of P. aeruginosa plus 1 fungal strain. Suspensions of P. aeruginosa and fungi were made in the solutions (1x10 6 colony-forming units/mL). After 1 hour (Arion Cronos only), 6, 8, and 24 hours, aliquots of suspension were removed and seeded on Luria-Bertani and Sabouraud agar plates. Results: After 6 hours' exposure, all the solutions but Dua Elite and Oxysept Comfort eradicated P. aeruginosa. Conversely, apart from 3% hydrogen peroxide-based Oxysept Comfort without catalase, which eradicated all the fungi tested after 6 hours, all the other solutions were partly ineffective at killing some of the fungal isolates, even after 24 hours' exposure. Conclusions: Most contact lens disinfectants may be ineffective if contact lens care systems become co-contaminated with P. aeruginosa and fungi. In our experiment, only exposure to 3% hydrogen peroxide without neutralizer for at least 6 hours was always able to kill a combined inoculum of P. aeruginosa and different fungal strains.

show abstract

“…until 2007 [23]. Despite the well-documented outbreak of Fusarium keratitis in 2006 [24], only a handful of studies have been conducted to evaluate the importance of fungal biofilms in CLAFK [23,[25][26][27][28]. This mini-review focuses on our current comprehension of the importance of biofilm formation in the pathogenesis and persistence of CLAFK.…”

Section: Introductionmentioning

confidence: 99%

The Role of Biofilms in Contact Lens Associated Fungal Keratitis

Yi,

Sun,

Zeng

et al. 2023

Antibiotics

View full text Add to dashboard Cite

Biofilm formation is an important microbial strategy for fungal pathogens, such as Fusarium, Aspergillus, and Candida, to establish keratitis in patients wearing soft contact lenses. Despite the well-documented 2006 outbreak of Fusarium keratitis that eventually led to the withdrawal of the Bausch & Lomb multipurpose lens care solution ReNu with MoistureLoc (“MoistureLoc”) from the global market, contact lens care systems and solutions currently available on the market do not specifically target fungal biofilms. This is partially due to the lack of recognition and understanding of important roles that fungal biofilms play in contact lens associated fungal keratitis (CLAFK). This review aims to reemphasize the link between fungal biofilms and CLAFK, and deepen our comprehension of its importance in pathogenesis and persistence of this medical device-related infection.

show abstract

Evaluations of Shorter Exposures of Contact Lens Cleaning Solutions against Fusarium oxysporum Species Complex and Fusarium solani Species Complex To Simulate Inappropriate Usage

Cited by 6 publications

References 37 publications

In Vitro Efficacy of Contact Lens Solutions Against Various Corneal Fungal Isolates

In Vitro Efficacy of Contact Lens Solutions Against Various Corneal Fungal Isolates

Susceptibility of various corneal fungal isolates and Pseudomonas aeruginosa to contact lens disinfecting solutions

The Role of Biofilms in Contact Lens Associated Fungal Keratitis

Contact Info

Product

Resources

About