Evaluations and Selections for High Isoflavone Black Soybean Mutants Induced by NaN<sub>3</sub> Treatment

Abstract

Sodium azide (NaN 3 ) (2 mM) was used to induce mutation in black soybean variety CRWD for improvement of isoflavone content. The individual selections were made in M6, M7 and M8 generations, respectively. Both high levels of isoflavones and medium seed size traits were used as selection criteria. Across three growth seasons, the selected five NaN 3 -induced mutants accumulated more total isoflavones (average increases of 5% to 25%) than their wild type variety CRWD. Four mutants yielded more (average increases of 17% to 25%) than CRWD. Both mutants SA-M-03 (yellow cotyledon) and SA-M-05 (green cotyledon) are rich in isoflavones and may be useful in food and other applications.

Effect of Ultraviolet C Irradiation on Isoflavone Concentrations in Different Cultivars of Soybean (Glycine max)

“…In a study where the isoflavone synthase gene was transformed to promote higher isoflavone concentrations, the total isoflavone concentration in the callus tissues of the transformed line was three- to sixfold higher than that in the control cell line [ 57 ]. In another study, NaN 3 -induced soybean mutants had higher total isoflavone concentrations (on average 5% to 25% higher) than their wild-type cultivar [ 58 ]. Furthermore, many studies have demonstrated that postharvest UV-C treatment in detached leaves, vegetables and fruits increases the secondary metabolite contents and their biosynthetic enzymes [ 59 , 60 , 61 , 62 , 63 , 64 ].…”

Selection of soybean mutant lines with altered seed coat colour and their antioxidant activity

“…Chemical mutagenesis using sodium azide (NaN3) in soybean induced genotypic variations that can influence the isoflavone content (Jeng et al. ). Here, we used gamma ray as a mutagen, and the selected mutant lines showed variation in their anthocyanin and isoflavone contents.…”

“…The content of isoflavones and anthocyanins in soybean seeds can vary according to the genotype (Kim et al 2005, Ha et al 2009). Chemical mutagenesis using sodium azide (NaN3) in soybean induced genotypic variations that can influence the isoflavone content (Jeng et al 2013). Here, we used gamma ray as a mutagen, and the selected mutant lines showed variation in their anthocyanin and isoflavone contents.…”