Evaluation The Success of Artificial Insemination Using Frozen Sexed Semen Based on Different Estrus Characters

Abstract

ABSTRACT. Knowledge of farmers about estrus detection is one of the factors that affect fixed-time artificial insemination (AI). This study aims to evaluate the success rate of AI using Y sexing frozen semen based on the estrus character of cattle. Forty-five Limousin Crossed Cows involved in this research with a Body Condition Score of 3-5 (1-9 scale), 1.8-7 years old. Y sexing frozen semen is produced by the Singosari Center for Artificial Insemination using Percoll's Gradient Density Centrifugation method. Observation of estrus character was done before insemination with the deep insemination technique of AI. Artificial insemination was carried out using double doses at the 2nd and 8th hours after estrus. Rheinbio vitamins as BioATP+ are injected after AI. The results showed that the Conception Rate and Pregnancy Rate based on the estrus character was higher as indicated by the red colour of the vulva was 17.78% and 22.22%; very swollen vulva was 20% and 22.22%; abundant cervical mucus was 15.56% and 17.78%, and vaginal temperatures ranging from 38.0-38.5oC was 13.33% and 20%. In conclusion, the low percentage of pregnancy in this study was caused by various factors, mainly the genetic quality, early embryonic efficacy, maintenance management especially feed, and there were cows that experienced reproductive disorders. (Evaluasi keberhasilan inseminasi buatan menggunakan semen beku sexing berdasarkan karakter estrus yang berbeda) ABSTRAK. Pengetahuan peternak mengenai deteksi estrus merupakan salah satu faktor yang berpengaruh terhadap ketepatan waktu inseminasi. Penelitian ini bertujuan untuk mengevaluasi tingkat keberhasilan IB menggunakan semen sexing Y berdasarkan karakter estrus ternak. Akseptor yang digunakan pada penelitian ini berjumlah 45 ekor Sapi Persilangan Limousin dengan BCS 3-5 dan umur berkisar antara 1,8-7 tahun. Semen beku sexing Y merupakan hasil produksi Balai Besar Inseminasi Buatan (BBIB) Singosari menggunakan metode Sentrifugasi Densitas Gradien Percoll (SGDP). Pengamatan karakter estrus dilakukan sebelum inseminasi. Inseminasi Buatan dilakukan dengan menggunakan double dosis pada jam ke-2 dan jam ke-8 dengan teknik deep insemination. Vitamin Rheinbio sebagai BioATP+ diinjeksikan setelah IB. Hasil penelitian menunjukkan bahwa keberhasilan kebuntingan ditinjau dari nilai conception rate (CR) dan pregnancy rate (PR) berdasarkan karakter estrus ternak lebih tinggi ditunjukkan pada kondisi warna vulva merah merata yaitu 17,78% dan 22,22%; vulva yang sangat bengkak yaitu 20% dan 22,22%; banyak mengeluarkan lendir servik yaitu 15,56% dan 17,78%, serta suhu vagina yang berkisar antara 38,0-38,5oC yaitu 13,33% dan 20%. Persentase kebuntingan yang rendah pada penelitian ini disebabkan oleh berbagai faktor, utamanya kualitas genetik ternak, kematian embrio dini, manajemen pemeliharaan khususnya pakan, dan terdapat ternak yang mengalami gangguan reproduksi.

Reproductive disorders of cows in several villages of Kedamean district, Gresik regency, East Java, Indonesia in 2023

“…Cases in Woha sub-district, Bima regency, West Nusa Tenggara province was 4.55% (Lukman et al, 2023), 10-40% in dairy farms (Lee et al, 2021), 19% in Tutur district, Pasuruan regency (Qodri et al, 2020. First ovulation in cows generally occurred silently (i.e., no estrus behavior) and was generally (>70%) followed by a short cycle (Utami et al, 2022). In this study, it was confirmed that some cows with SE at a young age (two to three years old, heifers or first parities).…”

“…Silent estrus could be caused by disruption of ovarian activity due to low consumption of nutrients in feed. Lack of nutrition caused endocrine disorders in the hypothalamus-pituitary-ovary axis, which was followed by low estrogen levels so that cows could not show signs of estrus (Utami et al, 2022). In this study, the highest number of SE cases occurred mostly in cows fed dry straw.…”

“…Silent estrus or sub-estrus occurred when the hormone estrogen was insufficient to manifest visual signs of estrus, even though the genital organs are undergoing normal cyclical changes (Crowe et al, 2014). The incidence of SE varies from 10% to 40% among different livestock groups (Utami et al, 2022). In this study cases of SE were 13.48% (126/935 cow population in the study area).…”

The success rate of artificial insemination in Limousin crossbred heifer with additional concentrate

“…One of the factors that can affect the success rate of artificial insemination is the physiological condition of livestock, such as the condition of cows in responding to the environment and adapting Utami et al [26]. Acceptors in this study had Limousin blood, a Bos Taurus cattle originating from hot areas.…”