Evaluation the effect of nicotine injection on the lungs of mice

Mohammed, Bushra Jasim; Al-Thwani, Amina N.

doi:10.4103/jrptps.jrptps_28_18

Cited by 2 publications

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“…One study found that the levels of GSH were reduced in the cerebellum, medulla oblongata, and hemisphere regions after young Wistar rats received a single dose of nicotine [78]. Another study with mice showed a significant decrease in GSH in the lungs after daily nicotine injections for several weeks [79]. Based on these studies, BDEVs could consist of key proteins associated with glutathione metabolism.…”

Section: Discussionmentioning

confidence: 99%

Role of Brain Derived Extracellular Vesicles in Decoding Sex Differences Associated with Nicotine Self-Administration

Koul

Schaal

Chand

et al. 2020

Cells

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Smoking remains a significant health and economic concern in the United States. Furthermore, the emerging pattern of nicotine intake between sexes further adds a layer of complexity. Nicotine is a potent psychostimulant with a high addiction liability that can significantly alter brain function. However, the neurobiological mechanisms underlying nicotine’s impact on brain function and behavior remain unclear. Elucidation of these mechanisms is of high clinical importance and may lead to improved therapeutics for smoking cessation. To fill in this critical knowledge gap, our current study focused on identifying sex-specific brain-derived extracellular vesicles (BDEV) signatures in male and female rats post nicotine self-administration. Extracellular vesicles (EVs) are comprised of phospholipid nanovesicles such as apoptotic bodies, microvesicles (MVs), and exosomes based on their origin or size. EVs are garnering significant attention as molecules involved in cell–cell communication and thus regulating the pathophysiology of several diseases. Interestingly, females post nicotine self-administration, showed larger BDEV sizes, along with impaired EV biogenesis compared to males. Next, using quantitative mass spectrometry-based proteomics, we identified BDEV signatures, including distinct molecular pathways, impacted between males and females. In summary, this study has identified sex-specific changes in BDEV biogenesis, protein cargo signatures, and molecular pathways associated with long-term nicotine self-administration.

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Section: Discussionmentioning

confidence: 99%

Role of Brain Derived Extracellular Vesicles in Decoding Sex Differences Associated with Nicotine Self-Administration

Koul

Schaal

Chand

et al. 2020

Cells

View full text Add to dashboard Cite

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“…Individuals who now smoke or have a history of smoking who are diagnosed with COPD face an elevated susceptibility to developing lung cancer, cardiovascular disease, and diabetes, according to [8]. Nicotine injection induced pathological changes in the lung tissues of mice, induces notable alterations in pulmonary tissues and levels of oxidant indicators, specifically malondialdehyde (MDA) and reduced glutathione (GSH), according to [9]. Oxidative stress (OS) arises from a perturbation in the equilibrium between the generation of Reactive Oxygen Species (ROS) and the protective mechanisms provided by antioxidant defense systems.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness of Sodium Thiosulfate and Resveratrol in Remodeling Lung Injury and Expression of BCL2 in Nicotine-Stressed Rats

Al-Tamemi,

Al-Okaily

2024

UPJOZ

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Background and Objective: Nicotine is linked to the development of several illnesses, including cancer The current study aimed to assess the ability of sodium thiosulfate (STS) to decrease the harmful effects of nicotine in lungs and gene expression of Bcl2 gene in rats and compare them with resveratrol (RES) supplement. Methods: Thirty-six male rats, Rattus norvegicus (weighing 190–220 g) were chosen randomly and separated into six equal groups. Animals in the control group were administered an intraperitoneal injection of normal saline, while those in group G1 were injected (I/P) with nicotine (1.5 mg/kg b.wt.). G2: were injected I/P with STS (450 mg/kg b.wt) and nicotine (1.5 mg/kg b.wt). G3: rats were gavaged with resveratrol supplement (87 mg/kg b.wt) and (I/P) injected with nicotine at the same dose. G4 group were (I/P) injected with STS (450 mg/kg b.wt) and administered (RES) (87 mg/kg b.wt) plus injected with nicotine (1.5 mg/kg b.wt). I/P injections of STS (450 mg/kg b.wt) and Resveratrol (87 mg/kg body weight) were given to group G5. All groups were administered for 28 days, after 24 hours after the last administration, all animals were sacrificed, and tissue specimens were collected from the lung for histopathological changes and for gene expression analysis of Bcl2. Results: The results of Lung sections for G1 showed severe peribronchiolar and perivascular lymphocytic cuffs, congestion, pulmonary emphysema, cuboidal metaplasia of the bronchiolar epithelium, granulomatous inflammation, thickening in the alveolar walls, and severe hemorrhage with hemosiderosis as compared with other experimental groups while group G2 showed that STS attenuating the effect of nicotine on lung tissue characterized by normal bronchioles which lined by normal columnar epithelium with scattered macrophages slight perivascular lymphocytic cuffs, a few desquamations of the bronchiolar epithelium, G3 group which administered RES showed that some alveoli had thickened walls; others showed slightly distended walls, few infiltrations of macrophages and lymphocytes, and desquamation of the bronchiolar epithelium. Moreover sections of lungs for G4 that administered both STS and RES with nicotine showed that most alveoli were normal, with slight thickening in their walls and slight blood vessel congestion. Furthermore, results represented a significant decrease in expression of the BCl2 gene for G1 compared with other groups. In contrast, a significant increase in the fold change of the BCL2 gene was observed in the G2, G3, G4, and G5 groups, indicating the protective effects of RES and STS on histopathological sections and fold change for the BCL2 gene against nicotine-stressed rats. Conclusion: Rats who received STS showed more effectiveness in modulating the bad effects of nicotine than those who received RES, which can give an idea that STS is more effective as an antioxidant than RES.

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Evaluation the effect of nicotine injection on the lungs of mice

Cited by 2 publications

References 0 publications

Role of Brain Derived Extracellular Vesicles in Decoding Sex Differences Associated with Nicotine Self-Administration

Role of Brain Derived Extracellular Vesicles in Decoding Sex Differences Associated with Nicotine Self-Administration

Effectiveness of Sodium Thiosulfate and Resveratrol in Remodeling Lung Injury and Expression of BCL2 in Nicotine-Stressed Rats

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