Evaluation of β-glucan particles as dual-function carriers for poorly soluble drugs

Šalamúnová, Petra; Saloň, Ivan; Ruphuy, Gabriela; Kroupová, Jiřina; Balouch, Martin; Hanuš, Jaroslav; Štěpánek, František

doi:10.1016/j.ejpb.2021.08.001

Cited by 12 publications

(3 citation statements)

References 31 publications

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“…Yeast glucan is mildly immunogenic, biodegradable, effectively phagocytosed and often used as a vehicle for drug delivery. [190][191][192] Due to the unique invasion route of being transported by the lymphatic system, yeastderived carriers have emerged as promising oral delivery vehicles. 193 A study to evaluate the availability of yeast dextran particles (GPs) as curcumin carriers was carried out by Jan Hosek's team, 194 who found that the curcumin-loaded GPs exerted pharmacological complex properties with antiinflammatory effects greater than those of pure GP or curcumin.…”

Section: Yeast-derived Systemsmentioning

confidence: 99%

Bioinspired and biomimetic strategies for inflammatory bowel disease therapy

Zhang,

Ye,

Zhu

et al. 2024

J. Mater. Chem. B

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Section: Yeast-derived Systemsmentioning

confidence: 99%

Bioinspired and biomimetic strategies for inflammatory bowel disease therapy

Zhang,

Ye,

Zhu

et al. 2024

J. Mater. Chem. B

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“…Although most cellular components of the original yeast are removed, GPs retain surface structural features that make them readily recognized by dectin-1 receptors of immune cells and actively phagocytosed. This property of GPs has been well documented both ex vivo , and in vivo. , Owing to their immunogenicity and porous nature, GPs lend themselves as vehicles for the encapsulation and targeted delivery of various bioactive substances. − Proposed diagnostic and therapeutic applications of GPs include their use as vaccine adjuvants, as immuno-active drug delivery systems for the treatment of inflammatory bowel disease, as a means of improving the bioavailability of poorly soluble drugs via lymphatic transport, or as contrast agents for imaging. , It has been recently shown that GPs injected into living Drosophila are rapidly distributed through the hemolymph and selectively taken up by macrophages without compromising their normal function …”

Section: Introductionmentioning

confidence: 99%

Magnetic Yeast Glucan Particles for Antibody-Free Separation of Viable Macrophages from Drosophila melanogaster

Krejčová,

Saloň,

Klimša

et al. 2023

ACS Biomater. Sci. Eng.

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Currently available methods for cell separation are generally based on fluorescent labeling using either endogenously expressed fluorescent markers or the binding of antibodies or antibody mimetics to surface antigenic epitopes. However, such modification of the target cells represents potential contamination by non-native proteins, which may affect further cell response and be outright undesirable in applications, such as cell expansion for diagnostic or therapeutic applications, including immunotherapy. We present a label-and antibody-free method for separating macrophages from living Drosophila based on their ability to preferentially phagocytose whole yeast glucan particles (GPs). Using a novel deswelling entrapment approach based on spray drying, we have successfully fabricated yeast glucan particles with the previously unachievable content of magnetic iron oxide nanoparticles while retaining their surface features responsible for phagocytosis. We demonstrate that magnetic yeast glucan particles enable macrophage separation at comparable yields to fluorescence-activated cell sorting without compromising their viability or affecting their normal function and gene expression. The use of magnetic yeast glucan particles is broadly applicable to situations where viable macrophages separated from living organisms are subsequently used for analyses, such as gene expression, metabolomics, proteomics, single-cell transcriptomics, or enzymatic activity analysis.

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“…Although the cell wall-derived β-1,3-D glucan has some shortcomings, such as low content (1–7%), high viscosity and complex production process ( 14 ). But the more importantly, β-1,3-D-glucan retains the ability to be recognized and phagocytosed, even after drug encapsulation ( 15 ). The β-1,3-D-glucan is the main pathogen-associated molecular pattern of fungi, and it can be recognized by the dectin-1 ( 16 ), complement receptor 3 (CR3) ( 17 ), and scavenger receptor ( 18 ), which makes it a targeting vector for macrophages.…”

Section: Introductionmentioning

confidence: 99%

Novel albendazole-glucan particles for enhancing intestinal absorption and improving hepatic targeting

Liu¹,

Yang²,

Zhu³

et al. 2022

Ann Transl Med

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Background: Glucan particles (GPs) are derived from the Saccharomyces cerevisiae cell wall. The hollow particles composed of β-1,3-D-glucan have been extensively studied in terms of immune regulation and macrophage-targeted drug delivery. Albendazole (ABZ) is a benzimidazole drug with good anti-parasitic activity and is the drug recommended by the World Health Organization for the first-line treatment of hydatid disease.Methods: A dynamic light scatterometer, scanning electron microscope, and transmission electron microscope were used to characterize the ABZ-GPs. High-Performance Liquid Chromatography (HPLC), Laser Scanning Confocal Microscope (LSCM), and an in vivo small animal imaging system were used to evaluate theability of ABZ-GPs to be recognized by macrophages, whether ABZ-GPs are more readily absorbed and eliminated in the blood than the original ABZ drug in rats, and the ability of ABZ-GPs to target the mouse liver. Results:The ABZ-GPs were successfully constructed to achieve fluorescence, magnetic resonance imagining, and laser confocal microscopy imaging. The glucan shell effectively protects ABZ from enzymatic degradation and from being pumped out in the gastrointestinal tract. The analysis of ABZ and its major metabolite albendazole sulfoxide in the rat plasma and mouse liver showed that compared to the ABZ suspension group, the degradation of ABZ-GPSs in the blood was low, and the targeting of ABZ-GPSs in the liver was significantly enhanced. Conclusions:In the oral treatment of hepatic hydatid disease, GPs can be used as carriers to achieve the targeted transport of ABZ, which in turn can be used for the targeted therapy of liver echinococcosis. Thus, ABZ-GPs may be a promising form of targeted therapy.

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Evaluation of β-glucan particles as dual-function carriers for poorly soluble drugs

Cited by 12 publications

References 31 publications

Bioinspired and biomimetic strategies for inflammatory bowel disease therapy

Bioinspired and biomimetic strategies for inflammatory bowel disease therapy

Magnetic Yeast Glucan Particles for Antibody-Free Separation of Viable Macrophages from Drosophila melanogaster

Novel albendazole-glucan particles for enhancing intestinal absorption and improving hepatic targeting

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