Evaluation of Xenotropic Murine Leukemia Virus and its R426Q Polymorphism in Patients with Prostate Cancer in Kerman, Southeast of Iran

Afshar, Reza Malekpour; Fahimeh, Gadari; Reza, Mollaie Hamid

doi:10.7314/apjcp.2012.13.8.3669

Cited by 5 publications

(5 citation statements)

References 22 publications

(22 reference statements)

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“…In addition, studies failing to detect XMRV or related MLVs in prostate cancer and CFS patients outnumber the studies confirming the original findings (Korn et al, 2012;Mi et al, 2012;Paolucci et al, 2012;Rezaei, Hearps et al, 2013). In a study the presence of XMRV was detected among some patients with prostate cancer in the Iran, but did not find any correlation between XMRV infection and various clinical pathological parameters of prostate cancer (Reza, Fahimeh et al, 2012).…”

Section: Discussionmentioning

confidence: 53%

“…Moreover neutralizing antibodies for XMRV were identified in 11 of 40 patients with prostate cancer and XMRV sequences were confirmed in five of 11 by nested PCR and fluorescence in situ hybridization (Arnold et al, 2010;Switzer et al, 2011). Recently a study reported that, XMRV was detectable in some patients with prostate cancer in Kerman, southeast of Iran (Reza et al, 2012). In contrast, in other studies have been found very low or no XMRV prevalence in prostate cancers in Europe (Germany, and the Netherlands) and USA using only RT-PCR (Fischer et al, 2008;Hohn et al, 2009;Aloia et al, 2010;Silverman et al, 2010;Sakuma et al, 2011;Sfanos, 2011;Stieler et al, 2011).…”

Section: Introductionmentioning

confidence: 92%

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No Detection of Xenotropic Murine Leukemia Virus-Related Viruses in Prostate Cancer in Sanandaj, West of Iran

Khodabandehloo

Hosseini²,

Rahmani³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Asian Pac J Cancer Prev, 14 (11), 6929-6933 IntroductionProstate cancer is the most common malignancy in men worldwide (Groom et al., 2012). The annual incidence rates of prostate cancer among Iranian population are dramatically higher than in other countries of Asia (Akbari et al., 2008). Multiple etiologies have been hypothesized for the cause of prostate cancers, including genetic defects, infectious agents and associated inflammation (Silverman et al., 2010). Identification of a potential virus as the cause of prostate cancer is very important because it could facilitate management of this disease such as treatment and prevention. A recently described retrovirus, the xenotropic murine leukemia virus-related virus (XMRV) was detected in about 40% of familial prostate cancer using a DNA microarray (Virochip) containing oligonucleotides comprising conserved sequences from known viral genomes and was strongly associated with homozygosity for the R462Q reduced activity, a variant of the antiviral enzyme, RNase L (Urisman et al., 2006).Murine leukemia viruses (MLVs) belong to gammaretroviruses in the retroviridae family, which viral RNA is reverse-transcribed to DNA during replication. They are endogenous viruses that have integrated their provirus DNA into the mouse genome and can cause leukemias, lymphomas, neurologic diseases, and has been reported to be detected in prostate cancer. However, this virus has not been detected in similar groups of patients in other studies. Herein, we sought to detect XMRV in prostate cancers and benign controls in Sanandaj, west of Iran. Materials and Methods: In a case-control study, genomic DNA was extracted from formalin fixed and paraffin embedded prostate tissues from a total of 163 Iranian patients. We developed a conventional and a nested PCR assay using primers targeting to an env specific sequence of XMRV. PCR assays were carried out on 63 prostate cancers and 100 benign prostate hyperplasias. Results: Beta-actin sequences were successfully detected in the DNA extracts from all prostate tissues, confirming DNA extraction integrity. We did not detect XMRV in samples either from prostate cancers or benign prostate hyperplasias using XMRV specific primers. Conclusions: We conclude that in our population XMRV does not play a role in genesis of prostate cancer.

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Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 92%

No Detection of Xenotropic Murine Leukemia Virus-Related Viruses in Prostate Cancer in Sanandaj, West of Iran

Khodabandehloo

Hosseini²,

Rahmani³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…It was suggested that XMRV prevalence in patients with CFS and patients with prostate cancer carrying a homozygous R462Q mutation in the RNaseL gene is 67% and 40%, respectively. [ 2 8 16 24 28 ]…”

Section: Discussionmentioning

confidence: 99%

“…The primers previously described were used for molecular detection via RT-PCR technique for these two targets [ Table 1 ]. [ 24 25 ] A total volume of 25 μL reaction mixture consisted of a 5-μM concentration of cDNA samples or controls, a deoxyribonucleoside triphosphates (dNTPs) mix (Fermentas, Germany) with equal concentrations of each of the four dNTPs at a 1mM concentration, each of forward and reverse primers at 1 μM concentration, MgCl 2 solution (Fermentas) at 1 μM concentration, 2.5 μM concentration PCR buffer 10x (Fermentas), and 0.5 units/μL concentration of Taq DNA polymerase (Fermentas), and sterilized distilled water was added to round out the total volume.…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

A survey on human T-cell lymphotropic virus type 1 (HTLV-1) and xenotropic murine leukemia virus-related virus (XMRV) coinfection in Tehran, Iran

et al. 2018

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Background:Xenotropic murine leukemia virus-related virus (XMRV) is a gamma retrovirus, which has been detected in patients with prostate cancer, chronic fatigue syndrome, and general population with a number of acquired infections such as infection with human T-cell lymphotropic virus (HTLV) and human immunodeficiency virus (HIV). The aim of this study was to determine the HTLV-1 and XMRV coinfection for the first time in Iranian patients who were admitted to the Tehran hospitals.Materials and Methods:Two hundred and ninety one patients suspected with HTLV-1 were referred to the hospitals affiliated to the Iran University of Medical Sciences, Tehran, Iran from April 2012 to October 2016. Genomic deoxyribonucleic acid (DNA)/ribonucleic acid (RNA) from peripheral blood mononuclear cells/cerebrospinal fluids was extracted by High Pure Viral Nucleic Acid Kit (Roche, Germany). After complementary DNA synthesis, conventional reverse transcriptase polymerase chain reaction was used for the detection of HTLV-1 or XMRV-infected patients. Statistical Package for Social Sciences (SPSS) software, version 16 (SPSS, Chicago, IL, USA) was used for statistical analyses.Results:Of the 291 patients suspected of HTLV infection, 123 (42.3%) were male with a mean age of 38±15 years. HTLV-1 RNA was found in 93 (31.9%) specimens comprising 40 men (41.3%) and 53 women (56.9%). Of the 93 patients who were HTLV-1 positive, one sample (1%) was positive for XMRV env gene.Conclusion:These findings suggest that the lack of significant detection of XMRV in patients who were HTLV-1 positive could not be associated with complications of HTLV-1. Although this is a preliminary report from Iranian patients with HTLV-1, further studies are needed to show the actual prevalence of XMRV infection by geographical distribution and various populations.

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“…In agreement with recent studies that also showed general absence of XMRV or detection of it in a small subset of cases ( 23 - 27 ), XMRV was not detected in our study groups, suggesting that the distribution of XMRV may vary in different geographic regions ( 24 ). In a study in Kerman, Iran, XMRV was detected only in 4% of patients with prostate cancer ( 31 ). The lack of XMRV in the present study may be due to population heterogeneity, as these patients referred to Sina Hospital from all over the country.…”

Section: Discussionmentioning

confidence: 99%

Xenotropic Murine Leukemia Virus-Related Virus and RNase L R462Q Variants in Iranian Patients With Sporadic Prostate Cancer

Babaei

Ahmadi

Rezaei

et al. 2015

Iran Red Crescent Med J

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Background:Although several studies have confirmed the association of xenotropic murine leukemia virus-related virus (XMRV) and prostate cancer, this association is still controversial, as most studies did not detect XMRV in prostate tissue samples. Furthermore, some genetic and epidemiological studies have highlighted a role for RNase L polymorphisms, particularly R462Q, in the progression of prostate cancer.Objectives:The focus of this study was on the association of XMRV and RNase L R462Q variants with the risk of prostate cancer in Iranian patients.Patients and Methods:In this case-control study, 40 and 80 individuals with sporadic prostate cancer and benign prostatic hyperplasia, respectively, were included. The presence of XMRV was evaluated by real-time polymerase chain reaction (PCR) of integrase and nested-PCR for the gag genes. The RNase L R462Q polymorphism analysis was carried out by PCR and sequencing.Results:In a total of 40 sporadic prostate cancer and 80 benign prostatic hyperplasia cases, no XMRV was detected by real-time PCR and nested-PCR. RNase L R462Q polymorphism analysis reveals that although there was an increase in the risk of prostate cancer correlated with the Q/Q allele of RNase L at position 462, the frequencies of the RNase L R462Q alleles were not statistically significant between the prostate cancer and benign prostatic hyperplasia groups (OR = 2.75 (95% CI = 0.67 - 11.3), P = 0.29).Conclusions:These results did not support the presence of XMRV in the samples with prostate cancer and showed that RNase L R462Q variants had relatively little or no impact on the risk of prostate cancer in Iranian population.

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Evaluation of Xenotropic Murine Leukemia Virus and its R426Q Polymorphism in Patients with Prostate Cancer in Kerman, Southeast of Iran

Cited by 5 publications

References 22 publications

No Detection of Xenotropic Murine Leukemia Virus-Related Viruses in Prostate Cancer in Sanandaj, West of Iran

No Detection of Xenotropic Murine Leukemia Virus-Related Viruses in Prostate Cancer in Sanandaj, West of Iran

A survey on human T-cell lymphotropic virus type 1 (HTLV-1) and xenotropic murine leukemia virus-related virus (XMRV) coinfection in Tehran, Iran

Xenotropic Murine Leukemia Virus-Related Virus and RNase L R462Q Variants in Iranian Patients With Sporadic Prostate Cancer

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