Evaluation of wheat landrace genotypes for salinity tolerance at vegetative stage by using morphological and molecular markers

Shahzad, Armghan; Ahmad, Munir; Iqbal, Muhammad; Ahmed, Iftikhar; Ali, Ghulam Muhammad

doi:10.4238/2012.march.19.2

Cited by 62 publications

(45 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Evaluation of plants to salt tolerance in hydroponics [22–24] and field conditions [25, 26] has been well documented. The hydroponic technique has unconditional advantages over field screening, because the saline conditions in the field may be heterogenous and environmental factors may mask the expression of salt tolerance.…”

Section: Introductionmentioning

confidence: 99%

Discerning morpho-anatomical, physiological and molecular multiformity in cultivated and wild genotypes of lentil with reconciliation to salinity stress

Singh

Kumari

et al. 2017

PLoS ONE

View full text Add to dashboard Cite

One hundred and sixty two genotypes of different Lens species were screened for salinity tolerance in hydroponics at 40, 80 and 120 mM sodium chloride (NaCl) for 30 d. The germination, seedling growth, biomass accumulation, seedling survivability, salinity scores, root and shoot anatomy, sodium ion (Na+), chloride ion (Cl-) and potassium ion (K+) concentrations, proline and antioxidant activities were measured to evaluate the performance of all the genotypes. The results were compared in respect of physiological (Na+, K+ and Cl-) and seed yield components obtained from field trials for salinity stress conducted during two years. Expression of salt tolerance in hydroponics was found to be reliable indicator for similarity in salt tolerance between genotypes and was evident in saline soil based comparisons. Impressive genotypic variation for salinity tolerance was observed among the genotypes screened under hydroponic and saline field conditions. Plant concentrations of Na+ and Cl- at 120 mM NaCl were found significantly correlated with germination, root and shoot length, fresh and dry weight of roots and shoots, seedling survivability, salinity scores and K+ under controlled conditions and ranked the genotypes along with their seed yield in the field. Root and shoot anatomy of tolerant line (PDL-1) and wild accession (ILWL-137) showed restricted uptake of Na+ and Cl- due to thick layer of their epidermis and endodermis as compared to sensitive cultigen (L-4076). All the genotypes were scanned using SSR markers for genetic diversity, which generated high polymorphism. On the basis of cluster analysis and population structure the contrasting genotypes were grouped into different classes. These markers may further be tested to explore their potential in marker-assisted selection.

show abstract

Section: Introductionmentioning

confidence: 99%

Discerning morpho-anatomical, physiological and molecular multiformity in cultivated and wild genotypes of lentil with reconciliation to salinity stress

Singh

Kumari

et al. 2017

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Several researchers have used different indices for measuring salt tolerance of crop plants. These include relative salt tolerance (Maas, 1986;Shahzad et al, 2012), stress susceptibility index (Fischer and Maurer, 1978), and absolute salt tolerance (Dewey, 1962). However, these methods of salt tolerance assessment include a series of mathematical procedures and calculations and are unable to show genotype × environment interactions.…”

Section: Discussionmentioning

confidence: 99%

Genetic variation in wheat germplasm for salinity tolerance atseedling stage: improved statistical inference

Hussain¹,

Khan²,

Ali³

2015

Turk J Agric For

View full text Add to dashboard Cite

Salinity is the major threat to global wheat production, particularly in arid and semiarid areas. Breeding salt-tolerant cultivars is one feasible solution, while the presence of genetic variation is a prerequisite for genetic improvement. To detect genetic variation for salt tolerance in wheat, a total of 150 wheat genotypes were tested for seedling-stage tolerance response at 300 mM NaCl in hydroponic culture. Significant differences (P ≤ 0.001) were identified in wheat for seedling traits, and 20 salt-tolerant and 5 susceptible genotypes were selected on the basis of root and shoot weights. These 25 genotypes were tested for salt-tolerance response at the early developmental stage in hydroponic culture at 0, 70, 140, 210, 280, and 350 mM NaCl salinity levels. GGEbiplot analysis was used for measuring salt tolerance for relative growth rates of root length, shoot length, and plant weight.

show abstract

“…, Shahzad et al. ). Screening under controlled conditions has given better results because of reduced environmental effects.…”

Section: Introductionmentioning

confidence: 99%

Genetic Variation for Salinity Tolerance in Pakistani Rice (Oryza sativa L.) Germplasm

Sakina

Ahmed

Shahzad

et al. 2015

J Agronomy Crop Science

Self Cite

View full text Add to dashboard Cite

Soil salinity is one of the major production constraints. Development and planting of salt‐tolerant varieties can reduce yield losses due to salinity. We screened 185 rice genotypes at germination stage in petri dishes under control, 50, 100 and 150 mm salt stress, and at seedling stage in Yoshida's hydroponic nutrient solution under control, 50 and 100 mm salt stress. At germination stage, 15 genotypes including Nona Bokra, Sonahri Kangni, 7421, 7423 and 7467, whereas at seedling stage, 28 genotypes including Nona Bokra, Jajai‐77, KSK‐133, KSK‐282, Fakhr‐e‐Malakand, Pakhal, IR‐6, Khushboo‐95, Shahkar and Shua‐92 were found salt tolerant. Basmati‐370, Mushkan, Homo‐46 and accessions 7436, 7437 and 7720 were sensitive to salinity at both germination and seedling stage. We further screened a subset of 33 salt‐tolerant and salt‐sensitive genotypes with SSR markers. Four SSR markers (RM19, RM171, RM172 and RM189) showed significant association with two or more of the studied traits under 50, 100 and 150 mm salt stress. These markers may be further tested for their potential in marker‐assisted selection. The salt‐tolerant genotypes identified in this study may prove useful in the development of salt‐tolerant rice varieties in adapted genetic background.

show abstract

Evaluation of wheat landrace genotypes for salinity tolerance at vegetative stage by using morphological and molecular markers

Cited by 62 publications

References 24 publications

Discerning morpho-anatomical, physiological and molecular multiformity in cultivated and wild genotypes of lentil with reconciliation to salinity stress

Discerning morpho-anatomical, physiological and molecular multiformity in cultivated and wild genotypes of lentil with reconciliation to salinity stress

Genetic variation in wheat germplasm for salinity tolerance atseedling stage: improved statistical inference

Genetic Variation for Salinity Tolerance in Pakistani Rice (Oryza sativa L.) Germplasm

Contact Info

Product

Resources

About