Evaluation of waterpipe smoke toxicity in C57BL/6 mice model

Abi-Gerges, Aniella; Dagher‐Hamalian, Carole; Abou-Khalil, Pamela; Chahine, Joe Braham; Hachem, Pia; Khalil, Christian

doi:10.1016/j.pupt.2020.101940

Cited by 7 publications

(2 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Harvested organs (lungs and liver) from 6 CON and 7 GE mice were fixed in 10% neutral buffered formalin for 48 h, dehydrated in 95% ethanol, and then embedded in paraffin as described previously (Abi‐Gerges et al, 2020; Zeeni et al, 2015). Briefly, two different sections of 5 μm thickness each were obtained from each organ and stained with H&E. All slides were evaluated blindly for pathological changes by an experienced board‐certified pathologist under a light microscope (Zeiss).…”

Section: Methodsmentioning

confidence: 99%

Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice

Nour‐Eldine

Sayyed

Harhous

et al. 2022

J of Applied Toxicology

Self Cite

View full text Add to dashboard Cite

Gasoline exposure has been widely reported in the literature as being toxic to human health. However, the exact underlying molecular mechanisms triggered by its inhalation have not been thoroughly investigated. We herein present a model of subchronic, static gasoline vapor inhalation in adult female C57BL/6 mice. Animals were exposed daily to either gasoline vapors (0.86 g/animal/90 min) or ambient air for 5 days/week over 7 consecutive weeks. At the end of the study period, toxic and molecular mechanisms underlying the inflammatory, oxidative, and apoptotic effects triggered by gasoline vapors, were examined in the lungs and liver of gasolineexposed (GE) mice.Static gasoline exposure induced a significant increase (+21%) in lungs/body weight (BW) ratio in GE versus control (CON) mice along with a pulmonary inflammation attested by histological staining. The latter was consistent with increases in the transcript levels of proinflammatory cytokines [Interleukins (ILs) 4 and 6], respectively by $ 6-and 4-fold in the lungs of GE mice compared to CON. Interestingly, IL-10 expression was also increased by $ 10-fold in the lungs of GE mice suggesting an attempt to counterbalance the established inflammation. Moreover, the pulmonary expression of IL-12 and TNF-α was downregulated by 2-and 4-fold, respectively, suggesting the skewing toward Th2 phenotype. Additionally, GE mice showed a significant upregulation in Bax/Bcl-2 ratio, caspases 3, 8, and 9 with no change in JNK expression in the lungs, suggesting the activation of both intrinsic and extrinsic apoptotic pathways. Static gasoline exposure over seven consecutive weeks had a minor hepatic portal inflammation attested by H&E staining along with an increase in the hepatic expression of the mitochondrial complexes in GE mice. Therefore, tissue damage biomarkers highlight the health risks associated with vapor exposure and may present potential therapeutic targets for recovery from gasoline intoxication.

show abstract

Section: Methodsmentioning

confidence: 99%

Gasoline fume inhalation induces apoptosis, inflammation, and favors Th2 polarization in C57BL/6 mice

Nour‐Eldine

Sayyed

Harhous

et al. 2022

J of Applied Toxicology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recent research revealed the presence of multiple dangerous toxins and carcinogens in its smoke and the lack of their filtration through the water. In addition, a study examining the underlying risks of subchronic WP exposure in mice showed increased urea serum levels, lungs, kidneys, bone marrow, and liver damage compared to controls [11]. In previous studies, a significant association of WPS with lung cancer was established; gastric and esophageal cancers were observed to have weak associations with WPS [12].…”

Section: Introductionmentioning

confidence: 99%

Waterpipe Smoking among Bladder Cancer Patients: A Cross-Sectional Study of Lebanese and Jordanian Populations

et al. 2021

View full text Add to dashboard Cite

Background. Bladder cancer (BC) is the second most reported cancer in Lebanon and the fifth in Jordan. Its risk factors are mainly smoking and occupational exposure to aromatic amines. In these countries where smoking and bladder cancer are highly prevalent, the role of waterpipe smoking (WPS) in bladder cancer is less investigated. We aim to compare two sets of patients between Lebanon and Jordan, focusing on their smoking habits, WP use, occupational exposure, and the grade/invasiveness of their bladder cancer. Methods. This is a cross-sectional study that compares the smoking culture between two sets of populations with bladder cancer, from two different countries. We recruited 274 bladder cancer patients over the 18 years of age at the American University of Beirut Medical Center (AUBMC), and 158 bladder cancer patients over the age of 18 years at the King Hussein Cancer Center (KHCC). Results. 7.7% of Lebanese patients had significantly more positive family history of bladder cancer compared to 13.9% of Jordanian patients ( p = 0.045 ). Another significant finding is that the majority of Lebanese patients 70.7% reported being frequently exposed to secondhand smoking, mainly cigarettes, versus only 48.6% of Jordanian patients ( p < 0.001 ). The increasing smoking trend among Lebanese females is remarkably the highest in the region, which contributed to the overall increase in smoking rates in the country. 17.1% of the Lebanese smoking patients are mainly but not exclusively WP smokers of which 6.3% are daily WP smokers, similarly 17.1% of the Jordanian patients of which 3.2% are daily WP smokers. There were 71.5% of Lebanese patients who had a noninvasive BC versus 40% of Jordanian patients ( p < 0.001 ), and more than one-third reported an occupational exposure to one of the risk factors of BC in both groups. Conclusions. Bladder cancer incidence is on the rise in both Jordan and Lebanon along with different smoking types. It is necessary to impose prevention policies to prevent and control the high smoking prevalence. Bladder cancer invasiveness is higher in Jordan compared to universal data.

show abstract