Walkers improve self-reliability. We examined the effectiveness of a newly developed wheeled walking frame for use by physically handicapped persons. Unstable gaits in walker users were analyzed by tri-axial accelerometers and a motion capture system. Several markers were placed on subjects' backs and legs. Subjects were requested to walk around a test course at a comfortable speed, while their motion was recorded by two high-speed video cameras. The activities performed on the test course comprised standing, normal walking, fast walking, and walking over a barrier. Any accidental falls were also recorded. We established the characteristic rules of gait motion using a walker. Furthermore, we demonstrated that gait characteristics are more conveniently extracted from acceleration sensors than from motion capture systems, since the sensors can be affixed to subjects for self-monitoring and goal achievements. The methods employing acceleration sensors are considered suitable for determining the average gait motions of elderly persons living in nursing homes, and can be used to evaluate walking motion before and after rehabilitation.