Evaluation of Volume and Total Nucleated Cell Count as Cord Blood Selection Parameters

Jaime‐Pérez, José Carlos; Monreal-Robles, Roberto; Rodríguez-Romo, Laura; Mancı́as-Guerra, Consuelo; Herrera‐Garza, José Luis; Gómez‐Almaguer, David

doi:10.1309/ajcpfb6exo7bjvlr

Cited by 34 publications

(39 citation statements)

References 29 publications

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“…The dose of total nucleated cells remains the chief determinant that impacts on the selection of umbilical cord blood units [17, 23, 24]. Recent research suggests that a TNC count of 1.5 × 10 9 in collected units is associated with a marked increase in likelihood of selection by transplant centres.…”

Section: Discussionmentioning

confidence: 99%

Modelling Improvements in Cell Yield of Banked Umbilical Cord Blood and the Impact on Availability of Donor Units for Transplantation into Adults

Kekre

Philippe²,

Mallick³

et al. 2013

Stem Cells International

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Umbilical cord blood (UCB) is used increasingly in allogeneic transplantation. The size of units remains limiting, especially for adult recipients. Whether modest improvements in the yield of cells surviving storage and thawing allow more patients to proceed to transplant was examined. The impact of improved cell yield on the number of available UCB units was simulated using 21 consecutive anonymous searches. The number of suitable UCB units was calculated based on hypothetical recipient weight of 50 kg, 70 kg, and 90 kg and was repeated for a 10%, 20%, and 30% increase in the fraction of cells surviving storage. Increasing the percentage of cells that survive storage by 30% lowered the threshold of cells needed to achieve similar engraftment rates and increased numbers of UCB units available for patients weighing 50 (P = 0.011), 70 (P = 0.014), and 90 kg (P = 0.003), controlling for differences in HLA compatibility. Moreover, if recipients were 90 kg, 12 out of 21 patients had access to at least one UCB unit that met standard criteria, which increased to 19 out of 21 patients (P = 0.035) when the fraction of cells surviving storage and thawing increased by 30%. Modest increases in the yield of cells in banked UCB units can significantly increase donor options for adult patients undergoing HSCT.

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Section: Discussionmentioning

confidence: 99%

Modelling Improvements in Cell Yield of Banked Umbilical Cord Blood and the Impact on Availability of Donor Units for Transplantation into Adults

Kekre

Philippe²,

Mallick³

et al. 2013

Stem Cells International

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“…Jaime-Pérez et al [66] analyzed 794 CB units in which the amount of CD34 + cells was determined by flow cytometry. Although there are not accepted universal guidelines, most Cord Blood Banks use the combination of product weight (volume) and total nucleated cell (TNC) count as the main selection factors for cryopreservation, requiring a TNC content from 6–10 × 10 8 for storage [67] and a minimal volume between 40 and 60 mL [68–70].…”

Section: Appropriate Cellular Dose To Be Transplantedmentioning

confidence: 99%

Toward Personalized Cell Therapies by Using Stem Cells: Seven Relevant Topics for Safety and Success in Stem Cell Therapy

Silva

Almeida

Rettore

et al. 2012

Journal of Biomedicine and Biotechnology

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Stem cells, both embryonic and adult, due to the potential for application in tissue regeneration have been the target of interest to the world scientific community. In fact, stem cells can be considered revolutionary in the field of medicine, especially in the treatment of a wide range of human diseases. However, caution is needed in the clinical application of such cells and this is an issue that demands more studies. This paper will discuss some controversial issues of importance for achieving cell therapy safety and success. Particularly, the following aspects of stem cell biology will be presented: methods for stem cells culture, teratogenic or tumorigenic potential, cellular dose, proliferation, senescence, karyotyping, and immunosuppressive activity.

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“…Desde la década de los ochentas se ha reconocido a la sangre de cordón umbilical (SCU) como una fuente rica en células madre hematopoyéticas, siendo estas de gran utilidad en el manejo y tratamiento de desórdenes hematológicos malignos y no malignos, desórdenes músculo esqueléticos, endocrinos, enfermedades cardiovasculares, inmunológicas, trastornos neurológicos, entre otros, razón por lo cual se considera hoy en día la necesidad de criopreservarla (1)(2)(3)(4)(5)(6)(7)(8). Aunque se observan muchas ventajas en comparación con las muestras obtenidas de médula ósea, la mayor desventaja es el bajo recuento de células progenitoras hematopoyéticas unido al volumen de la muestra obtenido (7-10).…”

Section: Introductionunclassified

“…Hasta el momento, se ha tenido en cuenta el volumen y el recuento total de células nucleadas como variables principales para la criopreservación de las muestras (6,(11)(12)(13)(14). El recuento de células CD34+ y el recuento total de células nucleadas han sido utilizados como parámetros para evaluar la calidad de la muestra (9), siendo el recuento de las células CD34+ el parámetro para evaluar el potencial hematopoyético de la muestra y el recuento total de células nucleadas el indicador de resultados de trasplantes y por ende de mortalidad (7,(15)(16)(17)(18)(19)(20)(21)(22).…”

Section: Introductionunclassified

“…El recuento de células CD34+ y el recuento total de células nucleadas han sido utilizados como parámetros para evaluar la calidad de la muestra (9), siendo el recuento de las células CD34+ el parámetro para evaluar el potencial hematopoyético de la muestra y el recuento total de células nucleadas el indicador de resultados de trasplantes y por ende de mortalidad (7,(15)(16)(17)(18)(19)(20)(21)(22). Un tratamiento efectivo requiere una muestra de SCU con aproximadamente 2 -2,5 X 107 células nucleadas por kg de peso (8,15,(23)(24)) y 2 X 105 -106 células CD34+ por kg de peso (6,19). Sin embargo, solamente el 25% de las muestras recolectadas con volúmenes mayores a 80 ml contienen un adecuado recuento celular para pacientes con pesos entre 50 y 70 kg (15).…”

Section: Introductionunclassified

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Correlación entre características físicas neonatales y maternas con el recuento total de células nucleadas y de células CD34+ por microlitro en sangre de cordón umbilical

et al. 2015

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ResumenIntroducción: La sangre de cordón umbilical (SCU) ha sido reconocida como una fuente de células madre hematopoyéticas. Múltiples estudios han sido realizados con el propósito de determinar variables maternas y neonatales que afecten el volumen, el recuento total de células nucleadas y de células CD34+. El presente estudio correlaciona variables maternas y neonatales con el recuento total de células nucleadas y de CD34+ medidas en µL (microlitro). Materiales y Métodos:Estudio correlacional en el que se analizaron 50 muestras de sangre de cordón umbilical de gestantes atendidas en dos IPS, una de Bogotá y otra de Ubaté, (Cundinamarca), durante un período de 7 meses del año 2013. La selección de estas muestras fue basada en un muestreo no probabilístico. Se calculó el coeficiente de correlación de Pearson con su respectiva significancia estadística entre las variables cuantitativas de la madre y del neonato, y el volumen, el recuento de células CD34+ y el recuento total de células nucleadas por µL. Resultados:Se encontró correlación positiva entre la longitud del cordón y el recuento total de células nucleadas. Así mismo entre el volumen inicial de la muestra y el recuento total de células nucleadas y el recuento de células CD34+ en µL y en mL (mililitro). Discusión:Múltiples estudios se han desarrollado entorno a la búsqueda de variables maternas y neonatales que afectan la calidad de la muestra. En Colombia no se habían descrito las correlaciones encontradas en el presente estudio. Es por ello, que el desarrollo de nuevos estudios con diseños analíticos será enriquecedor. Palabras claves: Células madre, antígeno CD34+, trasplante de células madre de sangre de cordón umbilical (SCU), neonatal, materno, factores de riesgo. CORRELATION BETWEEN NEONATAL AND MATERNAL PHYSICAL CHARACTERISTICS WITH TOTAL NUCLEATED CELL COUNT AND CELL COUNT PER MICROLITER CD34+ IN CORD BLOOD AbstractBackground: The cord blood has been recognized as a source of hematopoietic stem. Multiple studies have been conducted in order to determine maternal and neonatal variables that affect the volume, total nucleated cell count and CD34+ cells. This study correlated maternal and neonatal variables with the total nucleated cell count and CD34+ cells measured by µL. Materials and methods: Correlational study in which 50 samples of umbilical cord blood were analyzed of pregnant women at two health institutions, one at Bogotá and the other one at Ubaté, Cundinamarca, for a period of seven months of 2013. The selection of these samples was based on a non-probability sample. The Pearson correlation coefficient was calculated with their respective statistical significance between quantitative variables of mother and newborn, and the volume, the count of CD34+ cells and the total nucleated cell count measured by µL. Results:Positive correlation between the length of the umbilical cord and the total nucleated cell count was found. Similarly, correlation between total nucleated cell count with the initial volume and count of CD34+ cells in µL and m...

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Evaluation of Volume and Total Nucleated Cell Count as Cord Blood Selection Parameters

Cited by 34 publications

References 29 publications

Modelling Improvements in Cell Yield of Banked Umbilical Cord Blood and the Impact on Availability of Donor Units for Transplantation into Adults

Modelling Improvements in Cell Yield of Banked Umbilical Cord Blood and the Impact on Availability of Donor Units for Transplantation into Adults

Toward Personalized Cell Therapies by Using Stem Cells: Seven Relevant Topics for Safety and Success in Stem Cell Therapy

Correlación entre características físicas neonatales y maternas con el recuento total de células nucleadas y de células CD34+ por microlitro en sangre de cordón umbilical

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