Evaluation of Visceral Adipose Tissue Oxygenation by Blood Oxygen Level‐Dependent MRI in Zucker Diabetic Fatty Rats

Shi, Hong; Li, Yan Feng; Ji, Wen; Lin, Zhi Chun; Cai, Wei; Chen, Tao; Yuan, Bin; Niu, Xiu Long; Li, Han Ying; Shu, Wen; Li, Yu Ming; Yuan, Fang; Zhou, Xin; Zhang, Zhuoli

doi:10.1002/oby.22191

Cited by 3 publications

(2 citation statements)

References 42 publications

(73 reference statements)

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“…Body composition was assessed by magnetic resonance imaging (MRI) scan on gestational day 18. Mice were anesthetized with vaporized isoflurane (1.5%) to reduce respiratory motion artifacts and then imaged on a 3.0‐T MRI system (Magnetom Verio; Siemens, Erlangen, Germany) with an eight‐channel wrist coil as previously described . The volumes of the abdominal visceral WAT (vWAT, containing the perigonadal WAT [pWAT]) and subcutaneous WAT (scWAT, containing the inguinal WAT [iWAT]) were calculated for transverse sections via a manual threshold setting in ImageJ 1.41 (National Institutes of Health, Bethesda, Maryland).…”

Section: Methodsmentioning

confidence: 99%

β3‐Adrenergic Activation Improves Maternal and Offspring Perinatal Outcomes in Diet‐Induced Prepregnancy Obesity in Mice

Zhang

Miao

Chen

et al. 2019

Obesity

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Objective Prepregnancy obesity is an epidemic disorder that seriously threatens both maternal and offspring health. This study investigated the effects of β3‐adrenergic receptor (β3‐AR) activation on the perinatal outcomes in a diet‐induced prepregnancy obese (PPO) murine model. Methods Four‐week‐old female C57BL/6 mice were fed high‐fat diet or chow diet for 16 weeks to yield PPO mice and chow‐fed (CF) lean mice, respectively. After successful mating with CF males, the PPO and CF mice were both randomly divided into vehicle control‐ or CL316,243 (a highly selective β3‐AR agonist)‐treated groups. On gestational day 7, subcutaneous infusion of CL316,243 or saline vehicle (1 mg/kg/d) was provided using osmotic pumps. The perinatal outcomes, adipose tissue morphology, and metabolic and inflammatory markers were examined. Results Chronic β3‐AR agonist infusion induced brown adipose tissue activation and white adipose tissue browning and countered obesity‐induced alterations in lipid profiles, insulin resistance, and systemic and local inflammatory states. Moreover, β3‐AR activation was associated with improved placental perfusion and offspring outcomes. Conclusions Our results provide proof‐of‐principle evidence that pharmacological β3‐AR activation may be of therapeutic potential in preventing prepregnancy‐obesity‐associated adverse maternal and offspring perinatal outcomes.

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Section: Methodsmentioning

confidence: 99%

β3‐Adrenergic Activation Improves Maternal and Offspring Perinatal Outcomes in Diet‐Induced Prepregnancy Obesity in Mice

Zhang

Miao

Chen

et al. 2019

Obesity

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“…Moreover, diabetic rodent studies have found that R2* can be used to evaluate visceral adipose tissue oxygenation status (21). Thus, it is worth investigating whether R2* and T2 mapping can be used to evaluate the effectiveness of exercise prescription effectiveness.…”

Section: Introductionmentioning

confidence: 99%

Muscular blood oxygen level-dependent MRI is beneficial to evaluate effectiveness of an exercise prescription

et al. 2021

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Background: To determine the feasibility and validity of using blood oxygen level-dependent (BOLD) magnetic resonance imaging (MRI) to evaluate the effects of back extension exercise on core lumbar paraspinal muscle strength.Methods: In this prospective study, R2* and T2 mapping of paraspinal muscles of 100 healthy volunteers were performed before and after back extension exercises in different recovery sessions (session I, II, III or IV). Volunteers use the Roman chair to complete the back extension exercises. The cross-sectional area (CSA), R2* and T2 values were measured and analyzed in 3 muscles (iliocostalis, longissimus, and multifidus muscles) of the lower back before and after exercise. Results:The CSA and T2 values of iliocostalis, longissimus, and multifidus muscles at L3 and L4 levels were higher in recovery sessions I and II than in the resting-state (P<0.05); however, compared to that in the resting-state, the R2* value was significantly reduced in session I but increased in sessions II-IV (P<0.05).Furthermore, the CSA and T2 values in recovery session I were higher than those in the resting-state, whereas the R2* value was lower (P<0.05). After exercise, the recovery tendency of R2* and T2 value was consistent in both males and females, but a significant sex difference in R2* value was observed between recovery sessions III and IV (P<0.05).Conclusions: R2* mapping and T2 mapping are effective and feasible for assessment of the effects of back extension exercises on lumbar paraspinal muscle strength.

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NADPH Oxidase 2-Mediated Insult in the Auditory Cortex of Zucker Diabetic Fatty Rats

Wei

et al. 2019

Neural Plasticity

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Clinical data has confirmed that auditory impairment may be a secondary symptom of type 2 diabetes mellitus (T2DM). However, mechanisms underlying pathologic changes that occur in the auditory system, especially in the central auditory system (CAS), remain poorly understood. In this study, Zucker diabetic fatty (ZDF) rats were used as a T2DM rat model to observe ultrastructural alterations in the auditory cortex and investigate possible mechanisms underlying CAS damage in T2DM. The auditory brainstem response (ABR) of ZDF rats was found to be markedly elevated in low (8 kHz) and high (32 kHz) frequencies. Protein expression of NADPH oxidase 2 (NOX2) and its matching subunits P22phox, P47phox, and P67phox was increased in the auditory cortex of ZDF rats. Expression of 8-hydroxy-2-deoxyguanosine (8-OHdG), a marker of DNA oxidative damage, was also increased in the neuronal mitochondria of the auditory cortex of ZDF rats. Additionally, decreases in the mitochondrial total antioxidant capabilities (T-AOC), adenosine triphosphate (ATP) production, and mitochondrial membrane potential (MMP) were detected in the auditory cortex of ZDF rats, suggesting mitochondrial dysfunction. Transmission electron microscopy results indicated that ultrastructural damage had occurred to neurovascular units and mitochondria in the auditory cortex of ZDF rats. Furthermore, cytochrome c (Cyt c) translocation from mitochondria to cytoplasm and caspase 3-dependent apoptosis were also detected in the auditory cortex of ZDF rats. Consequently, the study demonstrated that T2DM may cause morphological damage to the CAS and that NOX2-associated mitochondrial oxidative damage and apoptosis may be partly responsible for this insult.

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Evaluation of Visceral Adipose Tissue Oxygenation by Blood Oxygen Level‐Dependent MRI in Zucker Diabetic Fatty Rats

Abstract: This work demonstrated the feasibility of BOLD-MRI for detecting the VAT oxygenation status in ZDF rats, and the BOLD-MRI signals were associated with insulin resistance and systemic metaflammation in ZDF rats during the development of obesity.

Cited by 3 publications

References 42 publications

β3‐Adrenergic Activation Improves Maternal and Offspring Perinatal Outcomes in Diet‐Induced Prepregnancy Obesity in Mice

β3‐Adrenergic Activation Improves Maternal and Offspring Perinatal Outcomes in Diet‐Induced Prepregnancy Obesity in Mice

Muscular blood oxygen level-dependent MRI is beneficial to evaluate effectiveness of an exercise prescription

NADPH Oxidase 2-Mediated Insult in the Auditory Cortex of Zucker Diabetic Fatty Rats

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