Evaluation of vero cell lysate antigen for the ELISA of flaviviruses

Ansari, M. Z.; Shope, Robert E.; Malik, Sohail

doi:10.1002/jcla.1860070408

Cited by 41 publications

(27 citation statements)

References 11 publications

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“…All sera were tested for the presence of anti-dengue IgG antibodies by ELISA as described previously [20] except that PEG-precipitated dengue virions (a mixture of all 4 serotypes) were used as the antigen and a peroxidase labeled anti-human Ig was used as the conjugate. A 1∶100 dilution of serum samples was used.…”

Section: Methodsmentioning

confidence: 99%

Dengue Serosurvey in Sint Eustatius

et al. 2014

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Four distinct serotypes of dengue viruses (DENV) are the cause of re-emerging dengue fever (DF) and dengue hemorrhagic fever (DHF). Dengue circulation in the Caribbean has gone from none or single serotype to multiple serotypes co-circulating with reports of continuing cycles of progressively more severe disease in the region. Few studies have investigated dengue on Sint Eustatius. Blood samples were collected to determine the prevalence of antibodies against dengue in the Sint Eustatius population. Greater than 90% of the serum samples (184 of 204) were positive for anti-flavivirus antibodies by enzyme linked immunosorbance assay (ELISA). Plaque reduction neutralization test (PRNT), specific for dengue viruses, showed that 171 of these 184 flavivirus antibody positive sera had a neutralization titer against one or more DENV serotypes. A majority of the sera (62%) had neutralizing antibody to all four dengue serotypes. Only 26 PRNT positive sera (15%) had monotypic dengue virus neutralizing antibody, most of which (20 of 26) were against DENV2. Evidence of infection with all four serotypes was observed across all age groups except in the youngest age group (10–19 years) which contained only DENV2 positive individuals. In a multiple logistic regression model, only the length of residence on the island was a predictor of a positive dengue PRNT50 result. To our knowledge this is the first dengue serosurveillance study conducted on Sint Eustatius since the 1970s. The lack of antibodies to the DEN1, 3, and 4 in the samples collected from participants under 20 years of age suggests that only DEN2 has circulated on island since the early 1990s. The high prevalence of antibodies against dengue (83.8%) and the observation that the length of time on the island was the strongest predictor of infection suggests dengue is endemic on Sint Eustatius and a public health concern that warrants further investigation.

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Section: Methodsmentioning

confidence: 99%

Dengue Serosurvey in Sint Eustatius

et al. 2014

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“…Dengue-specific IgG titers were determined by an IgG ELISA adapted from Ansari et al [29]. Plates (96-well format) were coated with DENV antigen (cocktail of serotypes 1, 2, 3 & 4) produced from infected Vero cell culture lysates or uninfected cell lysates as controls.…”

Section: Methodsmentioning

confidence: 99%

Epidemiology of Dengue Virus in Iquitos, Peru 1999 to 2005: Interepidemic and Epidemic Patterns of Transmission

et al. 2010

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BackgroundComprehensive, longitudinal field studies that monitor both disease and vector populations for dengue viruses are urgently needed as a pre-requisite for developing locally adaptable prevention programs or to appropriately test and license new vaccines.Methodology and Principal FindingsWe report the results from such a study spanning 5 years in the Amazonian city of Iquitos, Peru where DENV infection was monitored serologically among ∼2,400 members of a neighborhood-based cohort and through school-based absenteeism surveillance for active febrile illness among a subset of this cohort. At baseline, 80% of the study population had DENV antibodies, seroprevalence increased with age, and significant geographic variation was observed, with neighborhood-specific age-adjusted rates ranging from 67.1 to 89.9%. During the first 15 months, when DENV-1 and DENV-2 were co-circulating, population-based incidence rates ranged from 2–3 infections/100 person-years (p-years). The introduction of DENV-3 during the last half of 2001 was characterized by 3 distinct periods: amplification over at least 5–6 months, replacement of previously circulating serotypes, and epidemic transmission when incidence peaked at 89 infections/100 p-years.Conclusions/SignificanceNeighborhood-specific baseline seroprevalence rates were not predictive of geographic incidence patterns prior to the DENV-3 introduction, but were closely mirrored during the invasion of this serotype. Transmission varied geographically, with peak incidence occurring at different times among the 8 geographic zones in ∼16 km2 of the city. The lag from novel serotype introduction to epidemic transmission and knowledge of spatially explicit areas of elevated risk should be considered for more effective application of limited resources for dengue prevention.

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“…In addition, all samples initially typed by ELISA were reconfirmed by RT-PCR to distinguish among closely related viruses of the California and Bunyamwera serogroups (Armstrong et al 2005). For ELISA testing, antigen was harvested from infected Vero cell cultures and prepared as previously described (Ansari et al 1993). Viral antigens were titered in twofold dilutions from 1:10 to 1:1280 and identified using a panel of hyperimmune mouse ascitic fluids against JCV, La Crosse virus (LACV), Cache Valley virus (CVV), Highlands J virus (HJV), eastern equine encephalitis virus (EEEV), and West Nile virus (WNV), at a 1:10 dilution.…”

Section: Virus Isolation and Identificationmentioning

confidence: 99%

Isolations of Jamestown Canyon Virus (Bunyaviridae:Orthobunyavirus) from Field-Collected Mosquitoes (Diptera: Culicidae) in Connecticut, USA: A Ten-Year Analysis, 1997–2006

Andreadis

Anderson

Armstrong

et al. 2008

Vector-Borne and Zoonotic Diseases

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Jamestown Canyon virus (JCV) (Bunyaviridae: Orthobunyavirus) is a mosquito-borne zoonosis belonging to the California serogroup. It has a wide geographic distribution, occurring throughout much of temperate North America. White-tailed deer, Odocoileus virginianus are the principal amplification hosts, and boreal Aedes and Ochlerotatus mosquitoes are the primary vectors. A 10-year study was undertaken to identify potential mosquito vectors in Connecticut, quantify seasonal prevalence rates of infection, and define the geographic distribution of JCV in the state as a function of land use and white-tailed deer populations, which have increased substantially over this period. Jamestown Canyon virus was isolated from 22 mosquito species. Five of them, Ochlerotatus canadensis, Oc. cantator, Anopheles punctipennis, Coquillettidia perturbans, and Oc. abserratus were incriminated as the most likely vectors, based on yearly isolation frequencies and the spatial geographic distribution of infected mosquitoes. Jamestown Canyon virus was isolated from Oc. canadensis more consistently and from a greater range of collection sites than any other species. Frequent virus isolations were also made from Aedes cinereus, Aedes vexans, and Oc. sticticus, and new North American isolation records were established for Anopheles walkeri, Culex restuans, Culiseta morsitans, Oc. sticticus, Oc. taeniorhynchus, and Psorophora ferox. Other species from which JCV was isolated included C. melanura, Oc. aurifer, Oc. communis, Oc. excrucians, Oc. provocans, Oc. sollicitans, Oc. stimulans, Oc. triseriatus, and Oc. trivittatus. Jamestown Canyon virus was widely distributed throughout Connecticut and found to consistently circulate in a diverse array of mosquito vectors. Infected mosquitoes were collected from June through September, and peak infection rates paralleled mosquito abundance from mid-June through mid-July. Infection rates in mosquitoes were consistent from year to year, and overall virus activity was directly related to local mosquito abundance. Infected mosquitoes were equally distributed throughout the state, irrespective of land use, and infection rates were not directly associated with the abundance of white-tailed deer, possibly because of their saturation throughout the region.

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Evaluation of vero cell lysate antigen for the ELISA of flaviviruses

Cited by 41 publications

References 11 publications

Dengue Serosurvey in Sint Eustatius

Dengue Serosurvey in Sint Eustatius

Epidemiology of Dengue Virus in Iquitos, Peru 1999 to 2005: Interepidemic and Epidemic Patterns of Transmission

Isolations of Jamestown Canyon Virus (Bunyaviridae:Orthobunyavirus) from Field-Collected Mosquitoes (Diptera: Culicidae) in Connecticut, USA: A Ten-Year Analysis, 1997–2006

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