Evaluation of VEGF gene polymorphisms and proliferative diabetic retinopathy in Mexican population

González-Salinas, Roberto; García-Gutiérrez, M Carlota; García-Aguirre, Gerardo; Morales-Cantón, Virgilio; Vélez-Montoya, Raúl; Soberon, Vidal; González, Victoria; Lechuga, Rodrigo; García‐Solís, Pablo; García-Gutiérrez, David Gustavo; M, García-Solís; Viteri, Manuel Sáenz de; Solís-S, Juan Carlos

doi:10.18240/ijo.2017.01.22

Cited by 11 publications

(6 citation statements)

References 22 publications

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“…The increase of VEGF level alters capillary permeability, which contributes to the hemorrhage and exudation of retinal blood vessels, increase of angiopoietin production, formation of retinal neovascularization and visual impairment. Blocking VEGF completely prevents retinal neovascularization [ 28 ]. Inhibition of vascular growth factor can also reduce the formation of new blood vessels [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Sirtuin (Sirt) 3 Overexpression Prevents Retinopathy in Streptozotocin-Induced Diabetic Rats

et al. 2020

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Background Sirtuin (Sirt) 3 could promote autophagy by downregulating the expression of genes related to neovascularization in retinal endothelial cells. In this study, we aimed to investigate the effect of Sirt3 overexpression on retinopathy in streptozotocin (STZ)-induced diabetic rats, and to assess its mechanisms. Material/Methods Ntraperitoneal injection of STZ in rats was used to produce a diabetic model. The study rats were divided into 4 groups (n=6 for each group): a control group; a model group; a model+scrambled adenovirus group; and a model+Sirt3 overexpression group. Hematoxylin and eosin (H&E) staining determined the pathological changes of retina tissues. Immunohistochemistry, fluorescence quantitative polymerase chain reaction, and western blotting were used to detect the expression of Sirt3, vascular endothelial growth factor (VEGF), and microtubule-associated protein 1A/1B-light chain 3 (LC3). Results In the model group, the inner limiting membrane was swollen, uneven and thickened, and the capillary endothelial cells occasionally protruded into the inner limiting membrane. These abnormalities were prevented by Sirt3 overexpression. Compared with the control group, the expression of Sirt3 at both mRNA and protein levels in the model group was significantly reduced, while the expression of VEGF was increased versus the control group ( P <0.05). The expression of LC3 at both mRNA and protein levels was not different between the model group and the control group. Compared with the model group, the expression of Sirt3, LC3, and LC3-II was significantly increased, while the expression of VEGF was significantly decreased in the model+Sirt3 overexpression group ( P <0.05). Conclusions Sirt3 overexpression has a preventive effect on diabetic retinopathy likely through promoting the expression of autophagy-related proteins and downregulating the expression of VEGF.

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Section: Discussionmentioning

confidence: 99%

Sirtuin (Sirt) 3 Overexpression Prevents Retinopathy in Streptozotocin-Induced Diabetic Rats

et al. 2020

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“…Anti-VEGF therapy drugs have a short half-life, and retinal neovascularization may recur after a certain period of drug metabolism. Thus, the therapy needs to be repeated within a certain period of time [ 22 , 23 ].…”

Section: Related Workmentioning

confidence: 99%

Study on BCVA, CMT, CME, Curative Effect, and Prognostic Value of DR Sufferers Based on Surgical Therapy Combined with VEGF Therapy at Different Times

Wang

2022

Contrast Media & Molecular Imaging

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In order to explore the efficacy and prognostic value of best corrected visual acuity (BCVA), central macular retinal thickness (CMT), and cystoid macular edema (CME) in diabetic retinopathy (DR) sufferers based on surgical therapy combined with vascular endothelial growth factor (VEGF) therapy at different times, a total of 170 cases of DR sufferers who visited our hospital from March 2019 to March 2021 are analyzed. All patients are randomly divided into control group and study group, with 85 cases and 85 eyes in each group. In addition, the contrast set received 2 therapies for 1.5 months/time, and the study set received 3 therapies for 1 month/time. The experimental results show that the BCVA of the two sets is notoriously enhanced. Besides, CMT and CME in the study set decrease more than those in the contrast set, and the blood vessel density of retinal capillaries in the contrast set and the examination set is notoriously enhanced. For DR sufferers, 1 month/time of anti-VEGF therapy can effectively enhance the sufferers’ visual acuity, CMT and CME. It is suggested that the clinical application of 1 month/time of anti-VEGF to DR sufferers can enhance the clinical therapeutic effect of sufferers and reduce the prognosis and recurrence.

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“…Therefore, it is said that the inhibition of this factor has shown the improvement of these events at the level of the retina [103]. In a study conducted by Gonzalez-Salinas et al [109] in the Mexican population, they aimed to associate the polymorphisms rs3025035, rs3025021, and rs2010963 that just increase the expression of VEGF and that were previously associated with PDR in other populations; however, their results did not allow them to create a significant association. It requires new studies with a larger sample size, knowledge about pharmacological treatment, and fewer restrictions on the patient's clinical information which is highlighted [109].…”

Section: Pathophysiology Of Diabetic Retinopathymentioning

confidence: 99%

Oxidative Stress as the Main Target in Diabetic Retinopathy Pathophysiology

Olvera-Montaño

Alberto

Navarro-Partida

et al. 2019

Journal of Diabetes Research

109

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Diabetic retinopathy (DR) is one of the most common complications of diabetes mellitus (DM) causing vision impairment even at young ages. There are numerous mechanisms involved in its development such as inflammation and cellular degeneration leading to endothelial and neural damage. These mechanisms are interlinked thus worsening the diabetic retinopathy outcome. In this review, we propose oxidative stress as the focus point of this complication onset.

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Evaluation of VEGF gene polymorphisms and proliferative diabetic retinopathy in Mexican population

Cited by 11 publications

References 22 publications

Sirtuin (Sirt) 3 Overexpression Prevents Retinopathy in Streptozotocin-Induced Diabetic Rats

Sirtuin (Sirt) 3 Overexpression Prevents Retinopathy in Streptozotocin-Induced Diabetic Rats

Study on BCVA, CMT, CME, Curative Effect, and Prognostic Value of DR Sufferers Based on Surgical Therapy Combined with VEGF Therapy at Different Times

Oxidative Stress as the Main Target in Diabetic Retinopathy Pathophysiology

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