Evaluation of Vector Competence of Ixodes Ticks for Kemerovo Virus

Migné, Camille Victoire; Seixas, Hélène Braga de; Heckmann, Aurélie; Galon, Clémence; Jaafar, Fauziah Mohd; Monsion, Baptiste; Attoui, Houssam; Moutailler, Sara

doi:10.3390/v14051102

Cited by 2 publications

(4 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…KEMV titre was determined by plaque assay [ 8 ]. Cell culture plates (24-well) were seeded with BSR cells (1 × 10 5 cells/well) the day before titration.…”

Section: Methodsmentioning

confidence: 99%

“…ticks [ 3 , 4 ], field vectors of this virus have not been formally identified. We previously assessed the vector competence of artificially infected I. ricinus from France and Slovakia and I. persulcatus ticks from Russia for KEMV and showed that it infects French and Slovakian I. ricinus and Russian I. persulcatus [ 8 ]. The infection rates of engorged larvae were comparable for French and Slovakian I. ricinus larvae and nymphs and I. persulcatus larvae.…”

Section: Introductionmentioning

confidence: 99%

“…The infection rates of engorged larvae were comparable for French and Slovakian I. ricinus larvae and nymphs and I. persulcatus larvae. The rates of trans-stadial transmission were consistently higher in Slovakian I. ricinus and I. persulcatus [ 8 ]. French and Slovakian I. ricinus ticks which were infected at the larval stage became negative when tested by real-time RT-PCR four months post-moulting [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…The rates of trans-stadial transmission were consistently higher in Slovakian I. ricinus and I. persulcatus [ 8 ]. French and Slovakian I. ricinus ticks which were infected at the larval stage became negative when tested by real-time RT-PCR four months post-moulting [ 8 ]. In contrast, I. ricinus from Slovakia and I. persulcatus from Russia infected at the nymphal stage all remained positive for KEMV as post-moulting adult ticks.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Age- and Sex-Associated Pathogenesis of Cell Culture-Passaged Kemerovo Virus in IFNAR(−/−) Mice

Migné,

Heckmann,

Monsion

et al. 2024

IJMS

Self Cite

View full text Add to dashboard Cite

Kemerovo virus (KEMV) is a tick-borne orbivirus transmitted by ticks of the genus Ixodes. Previous animal experimentation studies with orbiviruses, in particular the interferon receptor double knock-out (IFNAR(−/−)) mouse model, did not indicate bias that is related to age or sex. We endeavoured to assess the effect of serial and alternated passages of KEMV in mammalian or Ixodes cells on virus replication and potential virulence in male or female IFNAR(−/−) mice, with important age differences: younger males (4–5 months old), older males (14–15 months old), and old females (14–15 months old). After 30 serial passages in mammalian or tick cells, or alternated passages in the two cell types, older female mice which were inoculated with the resulting virus strains were the first to show clinical signs and die. Younger males behaved differently from older males whether they were inoculated with the parental strain of KEMV or with any of the cell culture-passaged strains. The groups of male and female mice inoculated with the mammalian cell culture-adapted KEMV showed the lowest viraemia. While older female and younger male mice died by day 6 post-inoculation, surprisingly, the older males survived until the end of the experiment, which lasted 10 days. RNA extracted from blood and organs of the various mice was tested by probe-based KEMV real-time RT-PCR. Ct values of the RNA extracts were comparable between older females and younger males, while the values for older males were >5 Ct units higher for the various organs, indicating lower levels of replication. It is noteworthy that the hearts of the old males were the only organs that were negative for KEMV RNA. These results suggest, for the first time, an intriguing age- and sex-related bias for an orbivirus in this animal model. Changes in the amino acid sequence of the RNA-dependent RNA polymerase of Kemerovo virus, derived from the first serial passage in Ixodes cells (KEMV Ps.IRE1), were identified in the vicinity of the active polymerase site. This finding suggests that selection of a subpopulation of KEMV with better replication fitness in tick cells occurred.

show abstract

“…KEMV titre was determined by plaque assay [ 8 ]. Cell culture plates (24-well) were seeded with BSR cells (1 × 10 5 cells/well) the day before titration.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Age- and Sex-Associated Pathogenesis of Cell Culture-Passaged Kemerovo Virus in IFNAR(−/−) Mice

Migné,

Heckmann,

Monsion

et al. 2024

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

Hard Ticks as Vectors: The Emerging Threat of Tick-Borne Diseases in India

Perumalsamy,

Sharma,

Subramanian

et al. 2024

Pathogens

View full text Add to dashboard Cite

Hard ticks (Ixodidae) play a critical role in transmitting various tick-borne diseases (TBDs), posing significant global threats to human and animal health. Climatic factors influence the abundance, diversity, and vectorial capacity of tick vectors. It is imperative to have a comprehensive understanding of hard ticks, pathogens, eco-epidemiology, and the impact of climatic changes on the transmission dynamics of TBDs. The distribution and life cycle patterns of hard ticks are influenced by diverse ecological factors that, in turn, can be impacted by changes in climate, leading to the expansion of the tick vector’s range and geographical distribution. Vector competence, a pivotal aspect of vectorial capacity, involves the tick’s ability to acquire, maintain, and transmit pathogens. Hard ticks, by efficiently feeding on diverse hosts and manipulating their immunity through their saliva, emerge as competent vectors for various pathogens, such as viruses, parasites and bacteria. This ability significantly influences the success of pathogen transmission. Further exploration of genetic diversity, population structure, and hybrid tick vectors is crucial, as they play a substantial role in influencing vector competence and complicating the dynamics of TBDs. This comprehensive review deals with important TBDs in India and delves into a profound understanding of hard ticks as vectors, their biology, and the factors influencing their vector competence. Given that TBDs continue to pose a substantial threat to global health, the review emphasizes the urgency of investigating tick control strategies and advancing vaccine development. Special attention is given to the pivotal role of population genetics in comprehending the genetic diversity of tick populations and providing essential insights into their adaptability to environmental changes.

show abstract

Evaluation of Vector Competence of Ixodes Ticks for Kemerovo Virus

Cited by 2 publications

References 19 publications

Age- and Sex-Associated Pathogenesis of Cell Culture-Passaged Kemerovo Virus in IFNAR(−/−) Mice

Age- and Sex-Associated Pathogenesis of Cell Culture-Passaged Kemerovo Virus in IFNAR(−/−) Mice

Hard Ticks as Vectors: The Emerging Threat of Tick-Borne Diseases in India

Contact Info

Product

Resources

About