Evaluation of various parameters of calcium-alginate immobilization method for enhanced alkaline protease production by Bacillus licheniformis NCIM-2042 using statistical methods

Potumarthi, Ravichandra; Subhakar, Chennupati; A, Pavani; Jetty, Annapurna

doi:10.1016/j.biortech.2007.03.041

Cited by 55 publications

(25 citation statements)

References 30 publications

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“…Since single factor experiments cannot consider the interactions between various parameters, statistical methods including RSM have been widely applied for the optimization of the parameters for various biotechnology processes [7,15,16]. In the case of entrapment of microbial cells or enzymes, optimization of the immobilization conditions improves the rigidity and permeability of the beads [17].…”

Section: Optimization Of the Immobilization Conditionsmentioning

confidence: 99%

Alginate immobilization of recombinant Escherichia coli whole cells harboring l-arabinose isomerase for l-ribulose production

Zhang

Prabhu

Lee

2009

Bioprocess Biosyst Eng

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Recombinant Escherichia coli whole cells harboring Bacillus licheniformis L-arabinose isomerase (BLAI) were immobilized with alginate. The operational conditions for immobilization were optimized with response surface methodology. Optimal alginate concentration, Ca(2+) concentration, and cell mass loading were 1.8% (w/v), 0.1 M, and 44.5 g L(-1), respectively. The interactions between Ca(2+) concentration, alginate concentration, and initial cell mass were significant. After immobilization of BLAI, cross-linking with 0.1% glutaraldehyde significantly reduced cell leakage. The half-life of immobilized whole cells was 150 days, which was 50-fold longer than that of free cells. In seven repeated batches for L-ribulose production, the productivity was as high as 56.7 g L(-1) h(-1) at 400 g L(-1) substrate concentration. The immobilized cells retained 89% of the initial yield after 33 days of reaction. Immobilization of whole cells harboring BLAI, therefore, makes a suitable biocatalyst for the production of L-ribulose, particularly because of its high stability and low cost.

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Section: Optimization Of the Immobilization Conditionsmentioning

confidence: 99%

Alginate immobilization of recombinant Escherichia coli whole cells harboring l-arabinose isomerase for l-ribulose production

Zhang

Prabhu

Lee

2009

Bioprocess Biosyst Eng

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“…RSM was applied successfully for optimizing process parameters for various processes in biotechnology [16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Constitutive Expression and Optimization of Nutrients for Streptokinase Production by Pichia pastoris Using Statistical Methods

Vellanki

Potumarthi

Mangamoori

2008

Appl Biochem Biotechnol

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The Pichia pastoris clone producing streptokinase (SK) was optimized for its nutritional requirements to improve intracellular expression using statistical experimental designs and response surface methodology. The skc gene was ligated downstream of the native glyceraldehyde 3-phosphate dehydrogenase promoter and cloned in P. pastoris. Toxicity to the host was not observed by SK expression using YPD medium. The transformant producing SK at level of 1,120 IU/ml was selected, and the medium composition was investigated with the aim of achieving high expression levels. The effect of various carbon and nitrogen sources on SK production was tested by using Plackett-Burman statistical design and it was found that dextrose and peptone are the effective carbon and nitrogen sources among all the tested. The optimum conditions of selected production medium parameters were predicted using response surface methodology and the maximum predicted SK production of 2,136.23 IU/ml could be achieved with the production medium conditions of dextrose (x1), 2.90%; peptone (x2), 2.49%; pH, 7.2 (x3), and temperature, 30.4 (x4). Validation studies showed a 95% increase in SK production as compared to that before optimization at 2,089 IU/ml. SK produced by constitutive expression was found to be functionally active by plasminogen activation assay and fibrin clot lysis assay. The current recombinant expression system and medium composition may enable maximum production of recombinant streptokinase at bioreactor level.

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“…6) Furthermore, misinterpretation of results may occur if the interaction effects between different variables are overlooked. 25,26) These limitations, however, can be eliminated by using the response surface methodology (RSM) due to the latter's various advantages. 17,27) RSM is a collection of experimental strategies, and mathematical and statistical methods for constructing and exploring approximate functional relationships, between the response variable and a set of design variables.…”

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confidence: 99%

Optimization of Protease Extraction from Horse Mango (Mangifera foetidaLour) Kernels by a Response Surface Methodology

Ahmad

Liew

Yarmo

et al. 2012

Bioscience, Biotechnology, and Biochemistry

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Evaluation of various parameters of calcium-alginate immobilization method for enhanced alkaline protease production by Bacillus licheniformis NCIM-2042 using statistical methods

Cited by 55 publications

References 30 publications

Alginate immobilization of recombinant Escherichia coli whole cells harboring l-arabinose isomerase for l-ribulose production

Alginate immobilization of recombinant Escherichia coli whole cells harboring l-arabinose isomerase for l-ribulose production

Constitutive Expression and Optimization of Nutrients for Streptokinase Production by Pichia pastoris Using Statistical Methods

Optimization of Protease Extraction from Horse Mango (Mangifera foetidaLour) Kernels by a Response Surface Methodology

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