Evaluation of Various Lignocellulosic Products for the Cultivation of Shiitake Mushroom [Lentinula edodes (Berk.) Pegler]

Chittaragi, Amoghavarsha; Kumar, Ashwani; Muniraju, K.M.

doi:10.20546/ijcmas.2018.704.250

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“…The two selected strains are among the typical H- and L-type strains widely cultivated in northern and southern China. Mycelia were punched out using a cork borer (1 cm diameter), and then, were inoculated in Petri dishes (10 cm diameter) containing 35 mL of potato dextrose agar (PDA) medium as described previously [ 32 ]. Prior to inoculation, a sterilized cellophane membrane was placed on the surface of the PDA medium for easier collection of the mycelium samples.…”

Section: Methodsmentioning

confidence: 99%

Two Strains of Lentinula edodes Differ in Their Transcriptional and Metabolic Patterns and Respond Differently to Thermostress

Guo

Gao

Fan

et al. 2023

JoF

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Temperature type is one of the key traits determining the cultivation regime of Lentinula edodes. However, the molecular and metabolic basis underling temperature type remain unclear. Here, we investigated the phenotypic, transcriptomic, and metabolic features of L. edodes with different temperature types under both control (25 °C) and high (37 °C) temperature conditions. We found that under the control condition, the high- and low-temperature types of L. edodes harbored distinct transcriptional and metabolic profiles. The high-temperature (H-)-type strain had a higher expression level of genes involved in the toxin processes and carbohydrate binding, while the low-temperature (L-)-type strain had a high expression level of oxidoreductase activity. Heat stress significantly inhibited the growth of both H- and L-type strains, while the latter had a higher growth inhibition rate. Upon exposure to heat, the H-type strain significantly up-regulated genes associated with the components of the cellular membrane, whereas the L-type strain markedly up-regulated genes involved in the extracellular region and carbohydrate binding. Metabolome data showed that thermostress altered purine and pyrimidine metabolism in the H-type strain, whereas it altered cysteine, methionine, and glycerophospholipid metabolism in the L-type strain. Transcriptome and metabolome integrative analysis was able to identify three independent thermotolerance-related gene–metabolite regulatory networks. Our results deepen the current understanding of the molecular and metabolic basis underlying temperature type and suggest, for the first time, that thermotolerance mechanisms can be temperature-type-dependent for L. edodes.

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Section: Methodsmentioning

confidence: 99%