Evaluation of various cultural enrichment methods for the detection of selected food borne bacterial pathogens

Latha, C.; Sunil, B.; Kumar, Venkitanarayanan; Anu, C. J.; Deepa, J.

doi:10.14202/vetworld.2014.172-176

Vet World

2014

DOI: 10.14202/vetworld.2014.172-176

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Evaluation of various cultural enrichment methods for the detection of selected food borne bacterial pathogens

C. Latha¹,

B. Sunil²,

Venkitanarayanan Kumar³

et al.

Abstract: Aim:The study was conducted to evaluate the performance of different enrichment broths such as Tryptic Soy Yeast extract Broth (TSBYE), Brain Heart Infusion broth (BHI), Nutrient Broth (NB), Luria Broth (LB) and Peptone water (PW) for the detection of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus and Salmonella enterica Typhimurium. Materials and Methods:The bacterial strains were procured from Institute of Microbial Technology (IMTECH), 0 0Chandigarh. Growth of these food borne pathog… Show more

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Cited by 3 publications

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“…It could be concluded that the lab-made e-nose is able to detect rapidly the presence of bacteria L. monocytogenes and B. cereus on TSB media. For the future, it could be used to identify the presence of L. monocytogenes or B. cereus contamination in the routine and fast assessment of food products in animal quarantine.Listeria monocytogenes causes the highest case of hospitalization (up to 91%) among other foodborne illnesses [4]. Listeriosis is infectious to humans and mammals, including the ruminant and monogastric animals.…”

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confidence: 99%

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Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus

Astantri

Prakoso

Triyana

et al. 2020

Veterinary Sciences

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The aim of this study is to determine the performance of a lab-made electronic nose (e-nose) composed of an array of metal oxide semiconductor (MOS) gas sensors in the detection and differentiation of Listeria monocytogenes (L. monocytogenes) and Bacillus cereus (B. cereus) incubated in trypticsoy broth (TSB) media. Conventionally, the detection of L. monocytogenes and B. cereus is often performed by enzyme link immunosorbent assay (ELISA) and polymerase chain reaction (PCR). These techniques require trained operators and expert, expensive reagents and specific containment. In this study, three types of samples, namely, TSB media, L. monocytogenes (serotype 4b American Type Culture Collection (ATCC) 13792), and B. cereus (ATCC) 10876, were used for this experiment. Prior to measurement using the e-nose, each bacterium was inoculated in TSB at 1 × 10 3 -10 4 CFU/mL, followed by incubation for 48 h. To evaluate the performance of the e-nose, the measured data were then analyzed with chemometric models, namely linear and quadratic discriminant analysis (LDA and QDA), and support vector machine (SVM). As a result, the e-nose coupled with SVM showeda high accuracy of 98% in discriminating between TSB media and L. monocytogenes, and between TSB media and B. cereus. It could be concluded that the lab-made e-nose is able to detect rapidly the presence of bacteria L. monocytogenes and B. cereus on TSB media. For the future, it could be used to identify the presence of L. monocytogenes or B. cereus contamination in the routine and fast assessment of food products in animal quarantine.Listeria monocytogenes causes the highest case of hospitalization (up to 91%) among other foodborne illnesses [4]. Listeriosis is infectious to humans and mammals, including the ruminant and monogastric animals. The clinical signs of listeriosis in humans include gastroenteritis, diarrhea, meningitis, bacteremia, and it causes encephalitis, septicemia, abortion, mastitis, and gastroenteritis in cows [5,6]. Member of genus Listeria is a non-spore bacterium, being anaerobic facultative, a small size, Gram-positive, and rod-shaped (0.5-4.0 µm diameter and 0.5-2.0 µm long). Listeria monocytogenes can contaminate a wide range of foods, including yogurt, cheese, meat, ham, smoked salmon, poultry, seafood and vegetable products [2,7].Bacillus cereus is a facultative aerobic to anaerobic, Gram-positive, rod-shaped, and spore-forming bacteria. Spore endurance to unfavorable conditions has assisted the widespread of Bacillus [8,9]. Although the culture method is the gold standard for bacteria identification, it is inefficient, time-consuming (more than 1 week), requires laboratory operator expertise, and identification depends on specific microbiological and biochemical testing [7,9,10]. Besides these methods, there are also other detection methods such as polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). However, the PCR-based technique requires sophisticated equipment, complicated techniques, and lengthy processes such as ...

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mentioning

confidence: 99%

“…Listeria monocytogenes causes the highest case of hospitalization (up to 91%) among other foodborne illnesses [4]. Listeriosis is infectious to humans and mammals, including the ruminant and monogastric animals.…”

mentioning

confidence: 99%

Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus

Astantri

Prakoso

Triyana

et al. 2020

Veterinary Sciences

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Gas-generating reactions for point-of-care testing

Liu

Tian

Chen

et al. 2018

Analyst

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Gas generation-based measurement is an attractive alternative approach for POC (Point-of-care) testing, which relies on the amount of generated gas to detect the corresponding target concentrations. In gas generation-based POC testing, the integration of a target recognition component and a catalyzed gas-generating reaction initiated by the target introduction can lead to greatly amplified signals, which can be highly sensitive measured via distance readout or simple hand-held devices. More importantly, numerous gas-generating reactions are environment-friendly since their products such as oxygen and nitrogen are nontoxic and odourless, which makes gas generation-based POC testing safe and secure for inexperienced staff. Researchers have demonstrated that gas generation-based measurements enable the rapid and highly sensitive POC detection of a variety of analytes. In this review, we focus on the recent developments in gas generation-based POC testing systems. The common types of gas-generating reactions are first listed and the translation of gas signals to different signal readouts for POC testing are then summarized, including distance readouts and hand-held devices. Moreover, we introduce gas bubbles as actuators to power microfluidic devices. We finally provide the applications and future perspective of gas generation-based POC testing systems.

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Prevalence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using multiplex polymerase chain reaction

Latha¹,

Anu²,

Ajaykumar³

et al. 2017

Vet World

Self Cite

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Aim:The objective of the study was to investigate the occurrence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using the multiplex polymerase chain reaction (PCR) method.Materials and Methods:The assay combined an enrichment step in tryptic soy broth with yeast extract formulated for the simultaneous growth of target pathogens, DNA isolation and multiplex PCR. A total of 1134 samples including beef (n=349), chicken (n=325), pork (n=310), chevon (n=50), and meat products (n=100) were collected from different parts of Kerala, India. All the samples were subjected to multiplex PCR analysis and culture-based detection for the four pathogens in parallel.Results:Overall occurrence of L. monocytogenes was 0.08 % by cultural method. However, no L. monocytogenes was obtained by multiplex PCR method. Yersinia enterocolitica was obtained from beef and pork samples. A high prevalence of S. aureus (46.7%) was found in all types of meat samples tested. None of the samples was positive for S. Typhimurium.Conclusion:Multiplex PCR assay used in this study can detect more than one pathogen simultaneously by amplifying more than one target gene in a single reaction, which can save time and labor cost.

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Evaluation of various cultural enrichment methods for the detection of selected food borne bacterial pathogens

Cited by 3 publications

References 4 publications

Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus

Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus

Gas-generating reactions for point-of-care testing

Prevalence of Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus, and Salmonella enterica Typhimurium in meat and meat products using multiplex polymerase chain reaction

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