Evaluation of variables influencing the outcome of the atopy patch test

Langeveld-Wildschut, Elisabeth G.; Mariona, Ariënne M.W. van; Thepen, Theo; Mudde, Geert C.; Bruijnzeel, P. L. B.; Bruijnzeel-Koomen, Carla A.F.M.

doi:10.1016/s0091-6749(95)70034-x

Cited by 78 publications

(86 citation statements)

References 15 publications

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“…In studies performed on APT the percentages of positive subjects varied [7, 10, 14, 16], and this was ascribed to the absence of standardization in employed techniques and to the different purification and concentration of allergens [3, 9,16,17,18, 32]. At present, the technique most frequently employed is the application of extracts on healthy untreated upper back skin [3].…”

Section: Discussionmentioning

confidence: 99%

“…At present, the technique most frequently employed is the application of extracts on healthy untreated upper back skin [3]. The concentration and the purification of allergens are still discussed [3, 9,15,16,17,18, 32]. The main antigens of D. pteronyssinus and D. farinae have been isolated by feces or bodies (Der p 1 and Der f 1/Der p 2 and Der f 2), but most patch test studies were performed using whole mite culture or culture extracts, because of the high cost of purified allergens.…”

Section: Discussionmentioning

confidence: 99%

“…However, the outcome of the APT in subjects unaffected by AD has been poorly investigated with contrasting results. In 85 healthy volunteers no reactions to APT with house dust mites and grass pollens were observed by Langeveld-Wildschut et al [17]. No positive APT responses to aeroallergens in patients with respiratory atopy or controls were detected by Darsow et al [3].…”

Section: Discussionmentioning

confidence: 99%

“…Many studies on the APT have been performed [1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24], and it is regarded quite specific for patients with AD. However, small numbers of positive APT were reported in the past also in atopic subjects without dermatitis [6, 8,18,19,20, 23, 24] and in healthy persons [5, 9, 11, 13,18,19,20, 23, 24] and that would raise some question about the specificity and the clinical relevance of positive APT results.…”

Section: Introductionmentioning

confidence: 99%

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Is the Atopy Patch Test with House Dust Mites Specific for Atopic Dermatitis?

Ingordo

Nogare²,

Colecchia³

et al. 2004

Dermatology

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Background: The atopy patch test (APT), namely the patch test with aeroallergens, is regarded as specific for patients with atopic dermatitis (AD), but small numbers of positive APT were reported in the past also in atopic subjects without dermatitis and in healthy persons. Objective: The aim of this study was to evaluate the response to the APT with house dust mites (HDM) in subjects nonaffected by AD and to compare the outcomes observed in these cases with those pointed out in AD patients, evaluating also the differences between two allergen extracts manufactured at different purifications and concentrations. Methods: Forty-seven atopic subjects without eczema (AWE), 33 nonatopic (NA) subjects and 77 adult AD patients were patch tested with an extract of purified bodies of HDM at 20% and with another extract of whole bodies of HDM at 30%, the latter corresponding to 300 µg/g of Der p 1. The reproducibility of APT was also tested in 8 AD patients, in 37 AWE subjects and in 19 NA subjects. Results: Positive responses with extract at 20% were observed in 29 (37.7%) AD, in 5 (10.6%) AWE and in 4 (12.1%) NA subjects. The APT with HDM at 30% was positive in 32 (41.6%) AD, 9 (19.1%) AWE and 4 (12.1%) NA persons. The rates of positivity and the intensity scores of responses were significantly different between AD and non-AD subjects (p < 0.01). The reproducibility of the APT in the three groups was satisfactory. Conclusion: These observations lead to conclude that the APT with HDM is positive also in non-AD subjects but it is probably more specific for AD.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Is the Atopy Patch Test with House Dust Mites Specific for Atopic Dermatitis?

Ingordo

Nogare²,

Colecchia³

et al. 2004

Dermatology

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“…The activity of AD can also be influenced by allergens. Exposure to environmental allergens may aggravate the eczema by inhalation [12][13][14] or skin contact [15], and an improvement of the skin lesions can be seen after allergen avoidance [16][17][18]. Immunological evidence supporting the significance of allergen exposure comes from the presence of elevated serum IgE levels to aeroallergens, the binding of IgE to antigen presenting cells in the skin [19], and the presence of allergen specific T-cells in lesional AD skin [20,21].…”

mentioning

confidence: 99%

Bronchial and skin reactivity in asthmatic patients with and without atopic dermatitis

Brinkman¹,

Raaijmakers²,

Bruijnzeel-Koomen³

et al. 1997

Eur Respir J

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It is unclear whether the presence and severity of atopic dermatitis (AD) is predictive for the occurrence and severity of early and late asthmatic responses to inhaled allergens. The aim of this study was to compare the bronchial effects of allergen inhalation challenge in allergic asthma (AA) and atopic dermatitis.We therefore studied these responses in: nine patients with mild-to-moderate AA without AD; eight patients with mild-to-moderate AA and mild AD; eight patients with severe AD and mild AA; and eight patients with severe AD without AA. Allergen challenge was performed by inhaling doubling doses until the dose provoking a 20% fall in forced expiratory volume in one second (PC20) was reached. The late asthmatic reaction (LAR) was defined as a fall of >20% in peak expiratory flow (PEF) between 3 and 8 h after the challenge.All but four of the patients with severe AD without AA developed an early asthmatic response (EAR). A LAR was seen in all patients with severe AD and mild AA, in four patients with mild AD and mild-to-moderate AA, and in three patients with mild-to-moderate AA without AD. The LAR was most pronounced in patients with a combination of mild AA and severe AD. This could be explained, in part, by a decreased skin sensitivity in these patients, which made the Cockcroft formula for prediction of PC20 allergen less accurate in such patients.We conclude that patients with severe atopic dermatitis and mild asthma are at risk for developing pronounced late asthmatic responses after allergen exposure. This suggests that eosinophils activated in atopic dermatitis also predispose to airway inflammation.

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Role of Allergy Testing in Atopic Eczema

Darsow¹,

Ring²

Handbook of Atopic Eczema

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Evaluation of variables influencing the outcome of the atopy patch test

Cited by 78 publications

References 15 publications

Is the Atopy Patch Test with House Dust Mites Specific for Atopic Dermatitis?

Is the Atopy Patch Test with House Dust Mites Specific for Atopic Dermatitis?

Bronchial and skin reactivity in asthmatic patients with and without atopic dermatitis

Role of Allergy Testing in Atopic Eczema

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