Evaluation of vaccine candidates purified from the adult ticks of Ornithodoros savignyi (Acari: Argasidae) and Hyalomma dromedarii (Acari: Ixodidae) against tick infestations

Toaleb, Nagwa I.; Gabr, Hanan S. M.; El-Shafy, Sobhy Abd; Abdel-Rahman, Eman H.

doi:10.1007/s12639-018-01082-3

Cited by 6 publications

(6 citation statements)

References 41 publications

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“…For these reasons, alternative approaches to tick control have been suggested [ 18 ], of which vaccination or immunological control is regarded the most promising, environmentally friendly, and sustainable strategy. To date, numerous antigens have been reported to induce protection against Ixodid ticks [ 18 , 19 , 20 , 21 ] and less against Argasids [ 22 , 23 , 24 ]. Earlier, Willadsen [ 25 ] questioned whether combining antigens toward enhanced efficacy is a valid hypothesis.…”

Section: Introductionmentioning

confidence: 99%

“…Certainly, there are reasons for this slow progress which will not be addressed in this review. Therefore, it is not coincidental that to date only a few single vaccines against Argasids have been reported [ 22 , 23 , 24 ]. Nonetheless, tick researchers still aim to develop good vaccines which are able to induce a substantial humoral/or cell mediated protective immune responses in Argasid and Ixodid ticks.…”

Section: Introductionmentioning

confidence: 99%

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Cocktail Anti-Tick Vaccines: The Unforeseen Constraints and Approaches toward Enhanced Efficacies

Ndawula

Lew-Tabor

2020

Vaccines

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Ticks are second to mosquitoes as vectors of disease. Ticks affect livestock industries in Asia, Africa and Australia at ~$1.13 billion USD per annum. For instance, 80% of the global cattle population is at risk of infestation by the Rhipicephalus microplus species-complex, which in 2016 was estimated to cause $22–30 billion USD annual losses. Although the management of tick populations mainly relies on the application of acaricides, this raises concerns due to tick resistance and accumulation of chemical residues in milk, meat, and the environment. To counteract acaricide-resistant tick populations, immunological tick control is regarded among the most promising sustainable strategies. Indeed, immense efforts have been devoted toward identifying tick vaccine antigens. Until now, Bm86-based vaccines have been the most effective under field conditions, but they have shown mixed success worldwide. Currently, of the two Bm86 vaccines commercialized in the 1990s (GavacTM in Cuba and TickGARDPLUSTM in Australia), only GavacTM is available. There is thus growing consensus that combining antigens could broaden the protection range and enhance the efficacies of tick vaccines. Yet, the anticipated outcomes have not been achieved under field conditions. Therefore, this review demystifies the potential limitations and proposes ways of sustaining enhanced cocktail tick vaccine efficacy.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cocktail Anti-Tick Vaccines: The Unforeseen Constraints and Approaches toward Enhanced Efficacies

Ndawula

Lew-Tabor

2020

Vaccines

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“…To date, a significant number of antigens have been identified that offer protection against these ticks. The range of effective immune responses against argasid ticks is notably narrower compared to those for ixodid ticks [ 162 , 163 , 164 , 165 ]. Trager’s early work highlighted that guinea pigs and rabbits could develop immune resistance to ixodid tick larvae after repeated exposure [ 166 ].…”

Section: Advances In Anti-tick Vaccinesmentioning

confidence: 99%

“…Recent efforts, like the Bovimune Ixovac ® vaccine, aim to boost Bm86 vaccine effectiveness, but their efficacy remains unverified against ticks [ 269 ]. Despite the success of the Bm86 vaccine against R. microplus ticks, its applicability to other tick species like Ixodes remains uncertain due to differing physiological and genetic factors [ 164 , 177 , 270 , 271 ]. While the Bm86 vaccine has proven effective, its inability to eliminate ticks entirely limits its standalone use, yet it significantly reduces the need for acaricides, lessening the economic burden of tick-borne diseases in livestock [ 272 , 273 ].…”

Section: Advances In Anti-tick Vaccinesmentioning

confidence: 99%

Human Tick-Borne Diseases and Advances in Anti-Tick Vaccine Approaches: A Comprehensive Review

Nepveu-Traversy,

Fausther-Bovendo,

Babuadze

2024

Vaccines

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This comprehensive review explores the field of anti-tick vaccines, addressing their significance in combating tick-borne diseases of public health concern. The main objectives are to provide a brief epidemiology of diseases affecting humans and a thorough understanding of tick biology, traditional tick control methods, the development and mechanisms of anti-tick vaccines, their efficacy in field applications, associated challenges, and future prospects. Tick-borne diseases (TBDs) pose a significant and escalating threat to global health and the livestock industries due to the widespread distribution of ticks and the multitude of pathogens they transmit. Traditional tick control methods, such as acaricides and repellents, have limitations, including environmental concerns and the emergence of tick resistance. Anti-tick vaccines offer a promising alternative by targeting specific tick proteins crucial for feeding and pathogen transmission. Developing vaccines with antigens based on these essential proteins is likely to disrupt these processes. Indeed, anti-tick vaccines have shown efficacy in laboratory and field trials successfully implemented in livestock, reducing the prevalence of TBDs. However, some challenges still remain, including vaccine efficacy on different hosts, polymorphisms in ticks of the same species, and the economic considerations of adopting large-scale vaccine strategies. Emerging technologies and approaches hold promise for improving anti-tick vaccine development and expanding their impact on public health and agriculture.

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“…The prepared rabbit hyperimmune serum was defrosted and dialyzed for three days in a coupling buffer and then coupled to cyanogen bromide-activated Sepharose-4B (Sigma Aldrich) (2 mg/ml-swollen beads) according to the manufacturer's protocol. The C. parvum oocyst antigen was applied to the a nity column (Flex-Column, Kimble, USA) as described by Toaleb et al [59]. The bound fraction was eluted using the elution buffer.…”

Section: A Nity Puri Cation Of C Parvum Oocyst Antigenmentioning

confidence: 99%

A Cryptosporidium parvum vaccine candidate effect on immunohistochemical profiling of CD4, CD8, Caspase-3 and NF-κB in mice

Aboelsoued

Toaleb

Ibrahim

et al. 2022

Preprint

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Background: Cryptosporidium parvum is a protozoan parasite of medical and veterinary importance that causes neonatal diarrhea in many vertebrate hosts. In this study, we evaluated the efficacy of an affinity-purified antigen as a C. parvum vaccine candidate using ileal and liver tissues of experimentally infected neonatal mice by immunohistochemical profiling and immune scoring of CD4, CD8, Caspase-3, and nuclear factor kappa B (NF-κB). This vaccine was prepared from the C. parvum oocyst antigen using immunoaffinity chromatography with cyanogen bromide-activated Sepharose-4B beads. Thirty neonatal mice were divided into three groups (10 mice/group): (1) nonimmunized noninfected, (2) nonimmunized infected (using gastric tubes with a single dose of 1×105 of C. parvum oocysts in 250 µl PBS solution 1 h before a meal) and (3) immunized (twice with 40 µg/kg of purified C. parvum antigen at 2-week intervals and then infected with 1×105 C. parvum oocysts simultaneously with the second group). After euthanizing the animals on the 10th day, post-infection, their ileal and liver tissues were collected and prepared for immunohistochemistry (IHC) staining to detect CD4, CD8, Caspase-3, and NF-κB levels, which are indicators for T helper cells, cytotoxic T cells, apoptosis, and inflammation, respectively. Results: The IHC results showed that CD4, CD8, Caspase-3, and NF-κB antibodies varied significantly (P <0.001) in both organs in all the groups. We also recorded high CD4 levels and low CD8 antibodies in the nonimmunized noninfected mice tissues, while the opposite was observed in the nonimmunized infected mice tissues. In the immunized infected mice, the CD4 level was higher than CD8 in both organs. While the Caspase-3 levels were higher in the ileal tissue of nonimmunized infected than immunized infected mice ileal tissues, the reverse was seen in the liver tissues of both groups. Furthermore, NF-κB expression was higher in the liver tissues of nonimmunized infected mice than in immunized infected mice tissues. Therefore, the IHC results and immune-scoring program revealed a significant difference (P <0.001) in the CD4, CD8, Caspase-3, and NF-κB antibody levels in both ileal and liver tissues of all mice groups, which might be necessary for immunomodulation in these tissues. Conclusions: The improvement observed in the immunized infected mice suggests that this vaccine candidate might protect against cryptosporidiosis.

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Evaluation of vaccine candidates purified from the adult ticks of Ornithodoros savignyi (Acari: Argasidae) and Hyalomma dromedarii (Acari: Ixodidae) against tick infestations

Cited by 6 publications

References 41 publications

Cocktail Anti-Tick Vaccines: The Unforeseen Constraints and Approaches toward Enhanced Efficacies

Cocktail Anti-Tick Vaccines: The Unforeseen Constraints and Approaches toward Enhanced Efficacies

Human Tick-Borne Diseases and Advances in Anti-Tick Vaccine Approaches: A Comprehensive Review

A Cryptosporidium parvum vaccine candidate effect on immunohistochemical profiling of CD4, CD8, Caspase-3 and NF-κB in mice

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