Evaluation of UV damage at DNA level in Nicotiana plumbaginifolia protoplasts using single cell gel electrophoresis

Abas, Yulheri; Touil, Nadia; Kirsch‐Volders, Micheline; Angenon, Geert; Jacobs, M.; Famelaer, I.

doi:10.1007/s11240-007-9257-9

Cited by 8 publications

(4 citation statements)

References 31 publications

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“…In addition to CPD-mediated damage, UV-B also induces delay of G1-to-S phase transition within the plant cell cycle (Jiang et al, 2011). UV-C induces both single-stranded breaks and double-stranded breaks in Arabidopsis (Abas et al, 2007). Oxidative DNA damages are also found to be responsible for UV-associated mutagenicity and instability of plant genome (Manova and Gruszka, 2015).…”

Section: Genotoxic Stresses Leading To Instability In Plant Genomementioning

confidence: 99%

Tiny Yet Indispensable Plant MicroRNAs Are Worth to Explore as Key Components for Combating Genotoxic Stresses

Chowdhury

Basak

2019

Front. Plant Sci.

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Plants being sessile are always exposed to various stresses including biotic and abiotic stresses. Some of these stresses are genotoxic to cells causing DNA damage by forming lesions which include altered bases, cross-links, and breaking of DNA strands, which in turn hamper the genomic integrity. In order to survive through all these adverse conditions, plants have evolved different DNA repair mechanisms. As seen from the mammalian system and different human diseases, various microRNAs (miRNAs) can target the 3′-untranslated region of mRNAs that code for the proteins involved in DNA repair pathways. Since miRNAs play an important role in plant cells by regulating various metabolic pathways, it can also be possible that miRNAs play an important role in DNA repair pathways too. However, till date, only a handful of plant miRNAs have been identified to play important role in combating genotoxic stresses in plants. Limitation of information regarding involvement of miRNAs in DNA repair as well as in ROS scavenging prompted us to gather information about plant miRNAs specific for these tasks. This mini-review aims to present pertinent literature dealing with different genotoxic stresses that cause genome instability as well as plant specific responses to survive the damage. This is intertwined with the involvement of miRNAs in genotoxic stress in plants, challenges of applying miRNAs as a tool to combat DNA damage along with ways to overcome these challenges, and finally, the future prospective of these understudied aspects.

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Section: Genotoxic Stresses Leading To Instability In Plant Genomementioning

confidence: 99%

Tiny Yet Indispensable Plant MicroRNAs Are Worth to Explore as Key Components for Combating Genotoxic Stresses

Chowdhury

Basak

2019

Front. Plant Sci.

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“…However, by using a neutral comet assay (N/N variant), Roy et al ( 2011 ) showed that UV-B-induced lesions were reversible, particularly in A. thaliana wild-type (Col-0), compared to DNA polymerase λ UV-B sensitive mutants. UV-C was also shown to induce both SSBs and DSBs in Arabidopsis plumbaginifolia protoplasts (Abas et al, 2007 ). These authors also highlighted the usefulness of the comet assay as an analytical tool for the analysis of repair kinetics in protoplasts.…”

Section: Radiationmentioning

confidence: 99%

The use of comet assay in plant toxicology: recent advances

2015

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The systematic study of genotoxicity in plants induced by contaminants and other stress agents has been hindered to date by the lack of reliable and robust biomarkers. The comet assay is a versatile and sensitive method for the evaluation of DNA damages and DNA repair capacity at single-cell level. Due to its simplicity and sensitivity, and the small number of cells required to obtain robust results, the use of plant comet assay has drastically increased in the last decade. For years its use was restricted to a few model species, e.g., Allium cepa, Nicotiana tabacum, Vicia faba, or Arabidopsis thaliana but this number largely increased in the last years. Plant comet assay has been used to study the genotoxic impact of radiation, chemicals including pesticides, phytocompounds, heavy metals, nanoparticles or contaminated complex matrices. Here we will review the most recent data on the use of this technique as a standard approach for studying the genotoxic effects of different stress conditions on plants. Also, we will discuss the integration of information provided by the comet assay with other DNA-damage indicators, and with cellular responses including oxidative stress, cell division or cell death. Finally, we will focus on putative relations between transcripts related with DNA damage pathways, DNA replication and repair, oxidative stress and cell cycle progression that have been identified in plant cells with comet assays demonstrating DNA damage.

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“…Abas et al (2007) we expect that alternative tissues, such as immature pollen, will be relatively more applied in the future for MPP production, because of the lack of synchronization requirement, as demonstrated for Lilium and Spathiphyllum (Saito and Nakano 2002;Lakshmanan et al 2013). Moreover, MPPs could be selected based on filtration properties, which would result in different genome types, enabling researchers to attribute plant traits to particular chromosomes and further utilize MPPs accordingly.…”

Section: Advances In Protoplast Fusion and Fragmentationmentioning

confidence: 99%