Evaluation of Use of Recombinant Em18 and Affinity-Purified Em18 for Serological Differentiation of Alveolar Echinococcosis from Cystic Echinococcosis and Other Parasitic Infections

Xiao, Ning; Mamuti, Wulamu; Yamasaki, Hiroshi; Sako, Yasuhito; Nakao, Minoru; Nakaya, Kazuhiro; Gottstein, Bruno; Schantz, Peter; Lightowlers, Marshall W.; Craig, Philip S.; Itō, Akira

doi:10.1128/jcm.41.7.3351-3353.2003

Cited by 42 publications

(44 citation statements)

References 10 publications

(11 reference statements)

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“…These antigens have diagnostic sensitivities ranging between 81 and 100% and discriminate between AE and CE with a reliability ranging from 80 to C95% [15,26,27,[43][44][45][46]. The native and partially purified antigens tested in our study did not discriminate between AE and CE: they all recognised all AE and at least 81.3% of CE cases.…”

Section: Discussionmentioning

confidence: 88%

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

et al. 2011

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PURPOSE: Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. METHODS: The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. RESULTS: According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and >99.9% for the diagnosis of CE and AE, respectively. Antigens derived from E. multilocularis metacestodes had sensitivities ranging from 84 to 91% and >99.9% for the diagnosis of CE and AE, respectively. Specificities ranged from 92 to >99.9%. Post-test probabilities for the differential diagnosis of AE from liver neoplasias, CE from cystic liver lesions, and screening for AE in Switzerland were around 95, 86 and 2.2%, respectively. Cross-reactions with antibodies in sera of patients with other parasitic affections (fasciolosis, schistosomosis, amebosis, cysticercosis, and filarioses) did occur at variable frequencies, but could be eliminated through the use of confirmatory testing. CONCLUSIONS: Different metacestode-derived antigens of E. granulosus and E. multilocularis are valuable, widely accessible, and cost-efficient tools for the serological diagnosis of echinococcosis. However, confirmatory testing is necessary, due to the lack of species specificity and the occurrence of cross-reactions to other helminthic diseases. Abstract Purpose Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. Methods The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. Results According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and [99.9% for the diagnosis of CE and AE, respectively. Antigens derived from...

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Section: Discussionmentioning

confidence: 88%

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

et al. 2011

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“…Em18, an 18-kD antigen from E. multilocularis, is highly specific for detection of AE (Ito et al, 2003a;Xiao et al, 2003). An ELISA system using the recombinant Em18 antigen (RecEm18) could differentially distinguish AE from CE (Ito et al, 2003a) and may be used for monitering surgical and/or chemotherapeutic treatment and the follow up of AE patients after treatment (Fujimoto et al, 2005).…”

Section: Immunodiagnosis Of Alveolar Echinococcosis In Humansmentioning

confidence: 99%

Recent Advances in the Immunology and Serological Diagnosis of Echinococcosis

Zhang¹,

Li²,

Lin³

et al. 2012

Serological Diagnosis of Certain Human, Animal and Plant Diseases

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“…Therefore, immunological tests have been considered important methods to confirm clinical findings, to give diagnostic help by providing information on the parasite in case of unclear images, or to survey in areas of endemicity where imaging techniques are not readily available (4,9,11). Previously, we have reported an enzyme-linked immunosorbent assay (ELISA) and an immunoblot analysis (IB) by using recombinant E. multilocularis 18-kDa antigen (Em18), the breakdown product of ezrin-radixin-moesin-like protein (2) that is also known as EM10 (8), EM II/3 (7), or EM4 (10) by the cysteine peptidase, and demonstrated that these two tests have a high potential for differentially diagnosing AE (1,12,16,18). However, these two methods are time-consuming and require special materials and equipments, which make them not suitable for clinical applications.…”

Section: Alveolar Echinococcosis (Ae) Caused By the Larval Stage Ofmentioning

confidence: 99%

Development of an Immunochromatographic Test To Detect Antibodies against Recombinant Em18 for Diagnosis of Alveolar Echinococcosis

Sako

Fukuda²,

Kobayashi³

et al. 2009

J Clin Microbiol

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An immunochromatographic test (ICT) for the rapid detection of antibodies to Echinococcus multilocularis was developed. The ICT showed a sensitivity of 94% and a specificity of 95.4%. High degrees of agreement were observed between the ICT and an enzyme-linked immunosorbent assay ( ‫؍‬ 0.93) and between the ICT and immunoblot analysis ( ‫؍‬ 0.97). It is expected that the ICT developed in this study will be useful for the serodiagnosis of alveolar echinococcosis.

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Evaluation of Use of Recombinant Em18 and Affinity-Purified Em18 for Serological Differentiation of Alveolar Echinococcosis from Cystic Echinococcosis and Other Parasitic Infections

Cited by 42 publications

References 10 publications

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

Recent Advances in the Immunology and Serological Diagnosis of Echinococcosis

Development of an Immunochromatographic Test To Detect Antibodies against Recombinant Em18 for Diagnosis of Alveolar Echinococcosis

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