Evaluation of urdbean (Vigna mungo) genotypes for mungbean yellow mosaic virus resistance through phenotypic reaction and genotypic analysis

Abstract

Mungbean yellow mosaic virus (MYMV) is a whitefly-transmitted major destructive virus affecting urdbean productivity in India. The objective of this research was to identify urdbean genotypes resistant against MYMV based on the phenotypic reaction and genotypic analysis. A total 48 urdbean genotypes were evaluated for resistance to MYMV by visual scoring of symptoms in the field under natural conditions. Disease severity was assessed using 0-9 rating scale, according to the mean disease score, the urdbean genotypes were categorized into five groups resistant (R, 14 genotypes), moderately resistant (MR, 4 genotypes), moderately susceptible (MS, 10 genotypes), susceptible (S, 18 genotypes) and highly susceptible (HS, 2 genotypes). These results were confirmed through genotyping based on MYMV-resistance tagged molecular markers CEDG180, ISSR8111357 and YMV1 FR. In addition, biochemical analysis was carried out in the genotypes of each category (R-HS). Results showed that MYMV resistance was significantly and positively correlated with the phytic acid and total phenol contents, whereas negative correlation was observed with total sugars in susceptible genotypes. The new identified genotypes (resistant sources) can be utilized in the urdbean breeding programme for improving resistance to MYMV.

↵​Study of Morpho-physiological and Biochemical Traits for Resistance to Yellow Mosaic Virus (YMV) in Blackgram [Vigna mungo (L.) Hepper] Varieties

“…These compounds also directly act as activators of protective enzymes like polyphenol oxidases. In the current study, it was observed that the genotypes with high phenols content showed less incidence of YMV and the above results were corroborated with the studies of Tamilzharasi et al (2020), Rajitha et al (2018) and Vannirajan et al (2019) in blackgram.…”

The impact of insecticides and plant extracts on the suppression of insect vector (Bemisia tabaci) of Mungbean yellow mosaic virus (MYMV)

“…Disease incidence was evaluated by following the standard disease rating scale [ 25 , 26 ]. Response of different mungbean lines was determined based on percent disease infestation and lines were scored by using recommended 0–5 arbitrary scale ( Table 1 ) at reproductive stage [ 27 – 29 ].…”

Assessment of the Genetic Diversity of Black Gram [Vigna Mungo (L.) Hepper] Collections for Yellow Mosaic Virus Resistance Using Simple Sequence Repeat Markers

“…Pyngrope et al (2015) screened 30 black gram genotypes using 12 SSR markers and from the study, PIC value ranged from 0.22 to 0.68 and three polymorphic markers CEDG 180, CEDG 139 and CEDG 279 were identified. Tamilzharasi et al, (2018) genotyped 48 black gram lines for YMV resistance and tagged the molecular markers CEDG180, ISSR8111357 and YMV1 FR. In the present investigation SSR marker CEDG 139 had shown the PIC value of 0.59 (Table 6).…”