Evaluation of two diagnostic methods for dengue virus infection and its correlation with thrombocytopenia

Panwala, Tanvi; Mulla, Summaiya

doi:10.4103/2278-344x.180426

Cited by 2 publications

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“…On the other hand, association of thrombocytopenia with IgM was found to be higher in other studies. 20 This is probably due to the associated scare of dengue in the rainy months which has leads to early attendance in clinics, thus an early diagnosis. Studies claim that in addition to an early diagnosis, NS1 antigen may be an indicator of disease severity.…”

Section: Discussionmentioning

confidence: 99%

Utility of rapid diagnostic kit tests for diagnosis of suspected dengue fever

Otta

Swain

2019

Int J Res Med Sci

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Background: Dengue fever often presents as an undifferentiated febrile illness requiring a laboratory test for identification. Serological tests particularly on rapid kits for the detection of NS1Antigen, IgG and IgM antibodies are the most commonly performed test across the country.Methods: The serum samples of suspected dengue cases were tested by Rapid test kits for assessing all the three parameters as well as by ELISA for NS1 antigen test. The platelet count of the patients was obtained from automated coulter counter. The results thus obtained were analyzed in Excel format.Results: The serum samples from 304 suspected Dengue fever cases were received in the lab, of which 190 samples were positive either by rapid or ELISA and 176 when rapid card test was considered alone Highest seropositivity of dengue cases were observed in the age group of ≥60 years (79.2%) followed by 45-59 years (70.7%). On rapid test, 78 cases were NS1 antigen positive of which 60 cases were positive only for NS1 antigen. When NS1 rapid and ELISA tests when compared, 16 kit negative tests were positive on ELISA while 34 kit positive tests were ELISA negative. Sensitivity, specificity, PPV and NPV when only NS1 was considered on rapid test kits when compared with ELISA were 78.9%, 87.8%, 63.8% and 93.8%. 33.5% of serologically positive cases of Dengue had low platelet count on admission while only among negative cases 17.2% had a low platelet.Conclusions: Rapid kits often show variable results thus needing a validation of them from end user. As a positive dengue test result is an essential prerequisite for diagnosis thus it is essential that for serological tests ELISA technique should be used for reporting. Thus, it also mandates more efforts at decentralization of NVBDCP to include both government and non government institutions.

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Section: Discussionmentioning

confidence: 99%

Utility of rapid diagnostic kit tests for diagnosis of suspected dengue fever

Otta

Swain

2019

Int J Res Med Sci

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“…However, a fair number of patients were found positive by NS1 ELISA or IgM ELISA alone in our study, effective diagnosis of dengue requires testing by both. Panwala TH et al, reported higher positivity by IgM ELISA as compared to NS1 ELISA and also concluded that NS1 ELISA, if used in combination with IgM ELISA on a single serum sample of suspected patients, can improve the diagnosis of acute dengue cases and even treatment and control of dengue viral infection 35 .…”

Section: Association Of Vitamin-d Levels With Denguementioning

confidence: 99%

Untitled

2022

IJPSR

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Background: Dengue is an arthropod-borne viral disease caused by the dengue virus (DENV). DENV is an RNA virus that belongs to the Flavivirus genus. The incidence of dengue has grown in the recent decade globally. The World Health Organization (WHO) considers dengue as a major global public health challenge in the tropic and sub-tropic nations. Materials and Methods: NS1 antigen and IgM antibody ELISA were done, and the serum levels of Vitamin-D and Mannose Binding Lectins (MBL) were determined to find out their association with the severity of Dengue, i.e., Dengue fever (DF) / Dengue Haemorrhagic Fever status (DHF). Results: The male-to-female ratio was 1.35:1 with the most commonly affected age group of 11-20 years (31.3%). NS1 antigen ELISA alone was positive in 12 (15%) samples, IgM ELISA alone was positive in 20 (25%) samples, while NS1 and IgM ELISA both were positive in 48 (60%) samples. 64 (80%) and 16 (20%) cases were DF and DHF, respectively. Conclusion: Thrombocytopenia is significantly notable in DHF patients as compared to DF patients. Both NS1 and IgM ELISA are needed to effectively diagnose dengue cases. The association of low levels of MBL with DF and DHF might be related to the reduced activation of the MBL pathway of complement, leading to a higher viral load in dengue cases. INTRODUCTION: Dengue is an arthropod-borne viral disease caused by the Dengue virus (DENV).DENV is an RNA virus belonging to the genus Flavivirus. DENV changes genetically during natural transmission and significant biological differences have been seen between strains of the QUICK RESPONSE CODE

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Evaluation of two diagnostic methods for dengue virus infection and its correlation with thrombocytopenia

Cited by 2 publications

References 0 publications

Utility of rapid diagnostic kit tests for diagnosis of suspected dengue fever

Utility of rapid diagnostic kit tests for diagnosis of suspected dengue fever

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