Evaluation of tributyrin supplementation in milk replacer on diarrhoea occurrence in preweaning Holstein calves

Dell’Anno, Matteo; Scaglia, Elena; Reggi, Serena; Grossi, Silvia; Rossi, C.A. Sgoifo; Frazzini, Sara; Caprarulo, V.; Rossi, Luciana

doi:10.1016/j.animal.2023.100791

Cited by 5 publications

(7 citation statements)

References 50 publications

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“…High serum levels of DAO may occur following damage to the intestinal mucosa; hence, the DAO concentration can be used as an indirect marker of intestinal integrity [81]. A previous study suggested that serum DAO levels may be modulated by feed additive supplementation in preweaning calves [82]. Serum DAO titres suggest that the animal welfare levels and health status were generally correct, in line with the absence of diarrhoea occurrence at the end of the trial.…”

Section: Discussionmentioning

confidence: 96%

Effect of Dietary Supplementation of Chestnut and Quebracho Tannin Supplementation on Neonatal Diarrhoea in Preweaning Calves

Dell’Anno,

Frazzini,

Ferri

et al. 2024

Antioxidants

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Neonatal calf diarrhoea (NCD) poses a significant health challenge in cattle herds, resulting in considerable economic losses and antimicrobial use. In response to the escalating threat of antimicrobial resistance, viable alternatives are imperative, aligning with European policies. This study evaluated the in-milk supplementation of the chestnut and quebracho tannin extract in preweaning calves on performance, diarrhoea occurrence, Cryptosporidium spp. shedding, protein digestibility, and intestinal health. Twenty newborn calves were divided, after colostrum administration, into two experimental groups for 30 days as follows: the control (CTRL) was fed with whole milk and solid feed, and tannins (TAN) were fed whole milk supplemented with 6/g day of tannin extract and solid feed. Faecal samples were collected on days 0, 3, 7, 14, and 30 for the evaluation of Cryptosporidium oocyst shedding and protein digestibility. Faecal consistency was evaluated during the sampling using the faecal score scale (0–3 scale, considering diarrhoea > 1). The results showed a significant reduction in diarrhoea frequency in the TAN compared to the CTRL group (p < 0.05) over 30 days of the trial. The prevalence of Cryptosporidium spp. was generally low (12%), considering all analysed samples. Protein digestibility revealed comparable values for the TAN and CTRL groups, suggesting that tannins did not negatively affect milk protein availability. In conclusion, the in-milk supplementation of 6/g day of the chestnut and quebracho tannin extract could be considered a valuable functional feed additive to decrease NCD occurrence, thus supporting animal health and decreasing antibiotic use in livestock.

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Section: Discussionmentioning

confidence: 96%

Effect of Dietary Supplementation of Chestnut and Quebracho Tannin Supplementation on Neonatal Diarrhoea in Preweaning Calves

Dell’Anno,

Frazzini,

Ferri

et al. 2024

Antioxidants

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“…In rumen liquor samples of beeswax-supplemented groups compared to the control lambs, TVFAs increased significantly, while NH 3 -N and pH decreased. Rumen fermentation is a complex process involving animal physiology and microbiota [ 15 ]. Ruminants establish a symbiosis between host and microbial community, thus ensuring the supply of protein, vitamins, and fatty acids to satisfy the nutritional requirements.…”

Section: Discussionmentioning

confidence: 99%

“…The role of diet has a great impact on the quality of life, and the use of functional products with high concentrations of bioactive molecules of a natural origin is preferable to synthetic products [ 15 , 16 ]. Animal nutrition, through its functional role, is aimed at increasing performance and also supporting health status, thus decreasing the occurrence of pathologies and antibiotic use in livestock [ 15 ]. In ruminant nutrition, beeswax is an interesting by-product as a natural source of bioactive compounds which can impact positively on animal health.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Beeswax Supplementation on Productive Performance of Growing Assaf Lambs

Gaafar,

Dell’Anno,

Rossi

et al. 2023

Veterinary Sciences

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The aim of this work was to assess the effects of beeswax supplementation on growth rate, feed intake, nutrient digestion, rumen fermentation, blood parameters, and economic sustainability in Assaf lambs. Eighteen growing Assaf (5 months old) lambs were separated into three experimental groups (n = 6 lambs/group). The lambs were fed a basal diet without supplementation (G1) or supplemented with 2 and 4 g beeswax/head/day in G2 and G3 groups, respectively. Zootechnical performance was evaluated over a 90 day period. Feed digestibility was assessed in faeces through the acid insoluble-ash method, and rumen liquor was collected to measure ammonia (NH3-N) and total volatile fatty acid (TVFA) levels. Blood samples were obtained for the titration serum metabolites by colorimetric tests. The findings showed that G3 had an improved performance compared to the other groups (p < 0.01). The lambs in G3 revealed the highest nutrient digestibility and feed use, followed by G2, and G1. G3 recorded the highest economic efficiency followed by G2 and G1 (p < 0.01). The TVFA, acetate, and propionate concentrations were higher and the pH values, NH3-N, and butyrate concentrations were lower in G3 compared to G2 and particularly to G1 (p < 0.01). The concentrations of total protein, globulin, and glucose were significantly higher with 4 g beeswax (p < 0.05). However, albumin, cholesterol, total lipids, urea, creatinine, glutamic oxaloacetic transaminase (GOT), and glutamate pyruvate transaminase (GPT) concentrations as well as the albumin to globulin ratio decreased significantly with both levels of beeswax (p < 0.05). The addition of beeswax at the level of 4 g/head/day for growing Assaf lambs significantly improved the growth performance, digestibility, rumen fermentation, and blood serum parameters in addition to the economic efficiency.

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“…A fecal score ≤ 1 was considered normal, whereas a fecal > 1 was defined as diarrhea. Moreover, in order to monitor the animal welfare, the health status was also screened daily by observing the vitality of the animals [26]. Each animal was individually weighed once a week, and the feed intake was evaluated by measuring the feed refused.…”

Section: Animals Housing Experimental Design and Treatmentmentioning

confidence: 99%

“…This mineral in animal nutrition is involved in the activation of energy metabolism [42]. The hypometabolic profile represented by the whole cholesterol level was thus different among groups [26]; the treated group had a greater total cholesterol concentration than the control group. The higher levels in the treated group could result from higher systemic metabolic activity due to the mineral and functional characteristics of A. nodosum [43].…”

Section: Metabolic Profile Antioxidant Barrier and Immunoenzymatic An...mentioning

confidence: 99%

The Effects of Milk Replacer Supplemented with Ascophyllum nodosum as a Novel Ingredient to Prevent Neonatal Diarrhea in Dairy Calves and Improve Their Health Status

Scaglia,

Reggi,

Canala

et al. 2023

Veterinary Sciences

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Nutrition and health during pre-weaning affect the calves’ future fertility, calving age, production, and carrier length. Calves are highly susceptible to neonatal calf diarrhea (NCD), which can be fatal. NCD is due to hypovolemia and acidosis, which may involve anorexia and ataxia. The One Health principle calls for a drastic reduction in antimicrobial use. One approach is to improve animal health and reduce the use of antibiotics and functional ingredients that have beneficial effects due to bioactive compounds. Several functional ingredients and additives can be considered, and, in particular for this study, Ascophyllum nodosum was considered. The present study aimed to evaluate the role of A. nodosum as a functional ingredient implemented into the milk replacer in neonatal calves. Twelve pre-weaned Holstein Frisian calves, housed in twelve individual pens in the same environmental conditions, were divided into two groups of six animals: a control group (CTRL, n = 6) fed with a milk replacer, and a treatment group receiving milk enriched with 10 g of A. nodosum in their diet (TRT, n = 6) for 42 days. The fecal score was evaluated daily (3–0 scale) to monitor the incidence of diarrhea in the two groups. The body weight was evaluated weekly, and every two weeks feces were collected for microbiological evaluation using a selective medium for plate counting of total, lactic acid, and coliform bacteria. To verify the presence of Lactobacillus, Bifidobacterium, and Escherichia coli, real-time qPCR was used. At the beginning and at the end of the trial, blood samples were obtained for serum metabolite analysis. The growth performance did not differ in either of the two groups, but significant differences were observed in the incidence of moderate diarrhea (p-value < 0.0113), where the TRT group showed a lower incidence of cases during the 42-day period. Serum analysis highlighted higher contents of albumin, calcium, phosphorus, and total cholesterol in the TRT group compared to CTRL (p-value < 0.05). In conclusion, implementation of A. nodosum in the diet of calves can lead to better animal welfare and may reduce the use of antibiotics.

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Evaluation of tributyrin supplementation in milk replacer on diarrhoea occurrence in preweaning Holstein calves

Cited by 5 publications

References 50 publications

Effect of Dietary Supplementation of Chestnut and Quebracho Tannin Supplementation on Neonatal Diarrhoea in Preweaning Calves

Effect of Dietary Supplementation of Chestnut and Quebracho Tannin Supplementation on Neonatal Diarrhoea in Preweaning Calves

Evaluation of Beeswax Supplementation on Productive Performance of Growing Assaf Lambs

The Effects of Milk Replacer Supplemented with Ascophyllum nodosum as a Novel Ingredient to Prevent Neonatal Diarrhea in Dairy Calves and Improve Their Health Status

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