Evaluation of transportan 10 in PEI mediated plasmid delivery assay

Kilk, Kalle; El-Andaloussi, Samir; Järver, Peter; Meikas, Anne; Valkna, Andres; Bartfai, Tamás; Kogerman, Priit; Metsis, Madis; Langel, Ülo

doi:10.1016/j.jconrel.2004.12.006

Cited by 73 publications

(51 citation statements)

References 38 publications

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“…Correspondingly, it was suggested that the uptake of certain CPPs into eukaryotic cells is achieved without or with only transient membrane permeabilization, while CPPs may selectively disrupt prokaryotic membranes, thus resembling membrane-active AMPs (8,26,(40)(41)(42). Altogether, accumulating data suggest a dual effect of TP10: efficient cargo delivery into eukaryotic cells at submicromolar concentrations (43) and a membrane-disruptive activity toward microbial membranes at 4 to 8 M.…”

Section: Discussionmentioning

confidence: 99%

Identification of Cell-Penetrating Peptides That Are Bactericidal to Neisseria meningitidis and Prevent Inflammatory Responses upon Infection

Eriksson

Geörg

Sjölinder

et al. 2013

Antimicrob Agents Chemother

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bMeningococcal disease is characterized by a fast progression and a high mortality rate. Cell-penetrating peptides (CPPs), developed as vectors for cargo delivery into eukaryotic cells, share structural features with antimicrobial peptides. A screen identified two CPPs, transportan-10 (TP10) and model amphipathic peptide (MAP), with bactericidal action against Neisseria meningitidis. Both peptides were active in human whole blood at micromolar concentrations, while hemolysis remained negligible. Additionally, TP10 exhibited significant antibacterial activity in vivo. Uptake of SYTOX green into live meningococci was observed within minutes after TP10 treatment, suggesting that TP10 may act by membrane permeabilization. Apart from its bactericidal activity, TP10 suppressed inflammatory cytokine release from macrophages infected with N. meningitidis as well as from macrophages stimulated with enterobacterial and meningococcal lipopolysaccharide (LPS). Finally, incubation with TP10 reduced the binding of LPS to macrophages. This novel endotoxin-inhibiting property of TP10, together with its antimicrobial activity in vivo, indicates the possibility to design peptide-based therapies for infectious diseases.

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Section: Discussionmentioning

confidence: 99%

Identification of Cell-Penetrating Peptides That Are Bactericidal to Neisseria meningitidis and Prevent Inflammatory Responses upon Infection

Eriksson

Geörg

Sjölinder

et al. 2013

Antimicrob Agents Chemother

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“…2 and 3). The improvement of the in vitro transfection rate of plasmid DNAs after their attachment to natural peptides has been reported (43)(44)(45), but the resulting gene delivery systems generally lacked cell specificity. Here, we demonstrate that crotamine, which is a natural three-dimensional-structured peptide toxin, when employed as an in vitro gene delivery agent displays specificity to AP cells.…”

Section: Discussionmentioning

confidence: 99%

Crotamine Mediates Gene Delivery into Cells through the Binding to Heparan Sulfate Proteoglycans

Nascimento

Hayashi

Kerkis

et al. 2007

Journal of Biological Chemistry

103

119

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Recently we have shown that crotamine, a toxin from the South American rattlesnake Crotalus durissus terrificus venom, belongs to the family of cell-penetrating peptides. Moreover, crotamine was demonstrated to be a marker of centrioles, of cell cycle, and of actively proliferating cells. Herein we show that this toxin at non-toxic concentrations is also capable of binding electrostatically to plasmid DNA forming DNA-peptide complexes whose stabilities overcome the need for chemical conjugation for carrying nucleic acids into cells. Interestingly, crotamine demonstrates cell specificity and targeted delivery of plasmid DNA into actively proliferating cells both in vitro and in vivo, which distinguishes crotamine from other known natural cell-penetrating peptides. The mechanism of crotamine penetration and cargo delivery into cells was also investigated, showing the involvement of heparan sulfate proteoglycans in the uptake phase, which is followed by endocytosis and peptide accumulation within the acidic endosomal vesicles. Finally, the permeabilization of endosomal membranes induced by crotamine results in the leakage of the vesicles contents to the cell cytosol.

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“…NLS and scrambled peptides ( Figure 1A) of identical purity levels were obtained from Thermo Electron GmbH, (Ulm, Germany), also as Cterminal amides. In the reverse NLS peptide, the sequence corresponding to the nuclear localization signal of the SV40 large T antigen (amino acids [14][15][16][17][18][19][20] is inverted; the scrambled peptide was generated on the basis of S4 13 -PV peptide sequence, so that the resulting peptide had the same amino acid composition and overall charge, but a distinct primary sequence. Both peptides were also acetylated at their N-terminus.…”

Section: Cellsmentioning

confidence: 99%

“…Although the mechanisms underlying the cellular uptake of these peptides and of their conjugates remain highly debated, the efficient uptake of these peptides and, most importantly, the ability shared by a considerable number of cell penetrating peptides to accumulate inside the nucleus of cells, render them particularly suited to act as gene delivery vectors per se, or in association with other existing nonviral systems. Even though the delivery of peptides and proteins to the cytoplasm has been the major focus of research on cell penetrating peptides, a number of studies have focused on the use of cell penetrating peptides to mediate the intracellular delivery of plasmid DNA [15][16][17][18][19][20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

S4₁₃‐PV cell penetrating peptide and cationic liposomes act synergistically to mediate intracellular delivery of plasmid DNA

Trabulo

Mano

Faneca

et al. 2008

The Journal of Gene Medicine

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Background Cell penetrating peptides have been successfully used to mediate the intracellular delivery of a wide variety of molecules of pharmacological interest. The main aim of the present work was to evaluate the potential of the S4 13 -PV cell penetrating peptide to mediate the intracellular delivery of plasmid DNA, aiming at its use in gene therapy applications. The S4 13 -PV cell penetrating peptide is a chimeric peptide that results from the combination of a cell penetrating sequence derived from the Dermaseptin S4 peptide with the nuclear localization signal present in the Simian Virus 40 (SV40) large T antigen.

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Evaluation of transportan 10 in PEI mediated plasmid delivery assay

Cited by 73 publications

References 38 publications

Identification of Cell-Penetrating Peptides That Are Bactericidal to Neisseria meningitidis and Prevent Inflammatory Responses upon Infection

Identification of Cell-Penetrating Peptides That Are Bactericidal to Neisseria meningitidis and Prevent Inflammatory Responses upon Infection

Crotamine Mediates Gene Delivery into Cells through the Binding to Heparan Sulfate Proteoglycans

S4₁₃‐PV cell penetrating peptide and cationic liposomes act synergistically to mediate intracellular delivery of plasmid DNA

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Evaluation of transportan 10 in PEI mediated plasmid delivery assay

Cited by 73 publications

References 38 publications

Identification of Cell-Penetrating Peptides That Are Bactericidal to Neisseria meningitidis and Prevent Inflammatory Responses upon Infection

Identification of Cell-Penetrating Peptides That Are Bactericidal to Neisseria meningitidis and Prevent Inflammatory Responses upon Infection

Crotamine Mediates Gene Delivery into Cells through the Binding to Heparan Sulfate Proteoglycans

S413‐PV cell penetrating peptide and cationic liposomes act synergistically to mediate intracellular delivery of plasmid DNA

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S4₁₃‐PV cell penetrating peptide and cationic liposomes act synergistically to mediate intracellular delivery of plasmid DNA