Evaluation of transplantation of autologous bone marrow stromal cells into the cerebrospinal fluid for treatment of chronic spinal cord injury in dogs

Nishida, Hidetaka; Nakayama, Masanari; Tanaka, Hiroshi; Kitamura, Masaru; Hatoya, Shingo; Sugiura, Kikuya; Suzuki, Yoshihisa; Idé, Chizuka; Inaba, Toshio

doi:10.2460/ajvr.72.8.1118

Cited by 46 publications

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“…To the best of our knowledge, there have been only a few reports on the number of days until canine BMSCs reach confluency or the number of canine BMSCs collected in one passage [7,12]. In the present study, canine BMSCs grew to confluency at about 10 days, and approximately 3.8 × 10 6 BMSCs were collected per 2 ml of bone marrow in one passage.…”

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confidence: 63%

“…Some studies have shown that canine BMSCs can differentiate into neuron-like cells expressing mature neuronal markers [5,7,8]. For these reasons, our laboratory is currently investigating BMSCs for use in spinal cord regenerative therapy in dogs.Recently, the experimental study and clinical trial of regenerative therapy for SCI using autologous BMSCs also been performed in dogs [5,12]. Surprisingly, there have been almost no reports regarding basic research on canine BMSCs, although this is very important for the clinical application of canine BMSCs.…”

mentioning

confidence: 99%

“…Recently, the experimental study and clinical trial of regenerative therapy for SCI using autologous BMSCs also been performed in dogs [5,12]. Surprisingly, there have been almost no reports regarding basic research on canine BMSCs, although this is very important for the clinical application of canine BMSCs.…”

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confidence: 99%

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confidence: 99%

“…Recently, spinal cord regenerative therapy using stem cells has been clinically applied as one option for the treatment of severe SCI in human medicine [3,11,15]. Among the stem cells, bone marrow stromal cells (BMSCs) and olfactory ensheathing glia (OEG) have been the focus of research and clinical application for spinal cord regenerative therapy in dogs [5,7,8,12,14,17].BMSCs are adherent cells in the culture of bone marrow aspirates, but are not hematopoietic cells [13]. BMSCs are capable of differentiating into bone [2,6] BMSCs are easy to isolate in dogs and expand without serious technical problems [6].…”

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Proliferation Capacity, Neuronal Differentiation Potency and Microstructures after the Differentiation of Canine Bone Marrow Stromal Cells into Neurons

Edamura

Kuriyama

Kato

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRACT. We examined the proliferation capacity and neuronal differentiation potency of canine bone marrow stromal cells (BMSCs). In addition, the microstructures of neuron-like cells after neuronal differentiation were observed under a scanning electron microscope. Canine BMSCs grew to confluency at 10.0 ± 2.5 days, and 3.8 ± 2.1 × 10 6 BMSCs were collected in one passage. Approximately 65% of canine BMSCs changed to neuron-like morphology after neuronal differentiation, and nearly all neuron-like cells stained positive against neuron-specific enolase. In addition, microstructures such as the cellular organelles, filaments and growth cones of these cells bore a close resemblance to those of the original mature neurons. These results suggested that canine BMSCs might be capable of differentiating into neurons. KEY WORDS: bone marrow stromal cell, canine, differentiation, microstructure, neuron.doi: 10.1292/jvms.11-0388; J. Vet. Med. Sci. 74 (7): [923][924][925][926][927] 2012 Despite the progress in the treatment of spinal cord injury (SCI) in recent years, it is still difficult to recover from severe paralysis. Recently, spinal cord regenerative therapy using stem cells has been clinically applied as one option for the treatment of severe SCI in human medicine [3,11,15]. Among the stem cells, bone marrow stromal cells (BMSCs) and olfactory ensheathing glia (OEG) have been the focus of research and clinical application for spinal cord regenerative therapy in dogs [5,7,8,12,14,17].BMSCs are adherent cells in the culture of bone marrow aspirates, but are not hematopoietic cells [13]. BMSCs are capable of differentiating into bone [2,6] BMSCs are easy to isolate in dogs and expand without serious technical problems [6]. The most significant practical advantages of using BMSCs are that autologous transplantation is possible, the costs of culturing are lower, and the risk of teratoma formation is very low [13]. Some studies have shown that canine BMSCs can differentiate into neuron-like cells expressing mature neuronal markers [5,7,8]. For these reasons, our laboratory is currently investigating BMSCs for use in spinal cord regenerative therapy in dogs.Recently, the experimental study and clinical trial of regenerative therapy for SCI using autologous BMSCs also been performed in dogs [5,12]. Surprisingly, there have been almost no reports regarding basic research on canine BMSCs, although this is very important for the clinical application of canine BMSCs. Therefore, the purpose of the present study was to investigate the number of days until the canine BMSCs reach confluency and the number of cells collected in one passage. In addition, the percentage of total cells that changed to a neuron-like morphology and the percentage of cells shown to stain positive against a mature neuronal marker after neuronal differentiation were examined. We also observed the microstructures of the neuron-like cells differentiated from canine BMSCs under a scanning electron microscope (SEM).Six male beagle dogs (2.3 ± 1.8 years old...

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confidence: 63%

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confidence: 99%

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confidence: 99%

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confidence: 99%

mentioning

confidence: 99%

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Proliferation Capacity, Neuronal Differentiation Potency and Microstructures after the Differentiation of Canine Bone Marrow Stromal Cells into Neurons

Edamura

Kuriyama

Kato

et al. 2012

The Journal of Veterinary Medical Science

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Can Paraplegia by Disruption of the Spinal Cord Tissue Be Reversed? The Signs of a New Perspective

Branco

Alves

Pinheiro

et al. 2019

The Anatomical Record

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Central nervous system (CNS) trauma is often related to tissue loss, leading to partial or complete disruption of spinal cord function due to neuronal death. Although generally irreversible, traditional therapeutic efforts, such as physical therapy exercises, are generally recommended, but with a poor or reduced improvement of the microenvironment, which in turn stimulates neuroplasticity and neuroregeneration. Mesenchymal stem cells (MSCs) have paracrine, immunomodulatory, and anti‐inflammatory effects. Here we use stem cells to see if they can promote not only physical but also the functional regeneration of neuronal tissue in dogs with CNS traumas. Two dogs, one with chronic spinal cord injury and one with subacute spinal cord injury, underwent infusion of autologous MSCs in association with physiotherapy. The two treatments in combination were able to partially or completely recover the dog's walking movement again. The treatment of MSCs in association with physical therapy improved the microenvironment, which could be evidence of a paradigm shift that the CNS is not capable of functional regeneration after aggressive traumas. Anat Rec, 2019. © 2019 American Association for Anatomy Anat Rec, 303:1812–1820, 2020. © 2019 American Association for Anatomy

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Mesenchymal stem cell basic research and applications in dog medicine

Gugjoo

Amarpal

Sharma

2019

Journal Cellular Physiology

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The stem cells, owing to their special characteristics like self‐renewal, multiplication, homing, immunomodulation, anti‐inflammatory, and dedifferentiation are considered to carry an “all‐in‐one‐solution” for diverse clinical problems. However, the limited understanding of cellular physiology currently limits their definitive therapeutic use. Among various stem cell types, currently mesenchymal stem cells are extensively studied for dog clinical applications owing to their readily available sources, easy harvesting, and ability to differentiate both into mesodermal, as well as extramesodermal tissues. The isolated, culture expanded, and characterized cells have been applied both at preclinical as well as clinical settings in dogs with variable but mostly positive results. The results, though positive, are currently inconclusive and demands further intensive research on the properties and their dependence on the applications. Further, numerous clinical conditions of dog resemble to that of human counterparts and thus, if proved rewarding in the former may act as basis of therapy for the latter. The current review throws some light on dog mesenchymal stem cell properties and their potential therapeutic applications.

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Evaluation of transplantation of autologous bone marrow stromal cells into the cerebrospinal fluid for treatment of chronic spinal cord injury in dogs

Abstract: Injection of BMSCs into the CSF caused no complications and could have beneficial effects on pelvic limb locomotion in dogs with chronic spinal cord injuries.

Cited by 46 publications

References 38 publications

Proliferation Capacity, Neuronal Differentiation Potency and Microstructures after the Differentiation of Canine Bone Marrow Stromal Cells into Neurons

Proliferation Capacity, Neuronal Differentiation Potency and Microstructures after the Differentiation of Canine Bone Marrow Stromal Cells into Neurons

Can Paraplegia by Disruption of the Spinal Cord Tissue Be Reversed? The Signs of a New Perspective

Mesenchymal stem cell basic research and applications in dog medicine

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