Evaluation of trace elements and oxidative stress levels in the liver and kidney of streptozotocin-induced experimental diabetic rat model

Abstract

Abstract. In this study, we aimed to investage the relationship among trace elements (Cu, Fe, Zn and Mg) on oxidative and anti-oxidative substances in liver and kidneys tissues in streptozotocin (STZ) diabetic rat model. The mean levels of Fe and Cu were found significantly higher in the liver and kidneys of the diabetic rats, in comparison to the control rats. On the other hand, the mean levels of Zn and Mg in the liver and kidneys of the diabetic rats were significantly lower than in the control rats.The liver and kidneys malonaldehyde (MDA) levels of the experimental group were found to be higher than in the control group (p < 0.001; p < 0.01, respectively) after 4 weeks of the experimental period. Superoxide dismutase (SOD) activities and glutathione (GSH) levels in the liver tissue of STZ-induced diabetic rats were found to be lower in the experimental group than in the control group (p < 0.01). SOD activity and GSH concentration in kidneys of the diabetic rats were significantly diminished with respect to the control group (p < 0.01). In conclusion, the present results indicate that the increase of Fe and Cu together with decreas of Zn and Mg concentration in liver and kidney of STZ-induced diabetic rats may be involved in disturbances of oxidative balance in both the tissues. Therefore, these findings may contribute to explain the role of impaired ion metabolism of some elements in the progression of diabetic oxidative complications.

Dissecting copper homeostasis in diabetes mellitus

“…This theory was confirmed by many studies investigating the relationship between Cu/Zn‐SOD and T1D. Hepatic and renal Cu/Zn‐SOD activities from STZ‐diabetic rats were lower than the control condition. Furthermore, cardiac Cu/Zn‐SOD activity presented higher activity .…”

“…Although compilation of the results is difficult to achieve, one general view about alteration in Cu balance is widely accepted in the literature: T1D significantly elevated Cu content in kidney , liver , femur, erythrocyte, and lymphocyte in STZ‐treated rats . However, 180 days after the STZ‐injection, the renal Cu concentration of diabetic rats was similar to the control group .…”

Influence of Vanadium–organic Ligands Treatment on Selected Metal Levels in Kidneys of STZ Rats

“…The most important role of the kidney is water and mineral recovery and elimination of nitrogen metabolites. High urine production by diabetic rats can cause loss of vital elements, which can increase the organism dysfunction [38]. Vanadium administration in rats evidently increases the level of this element in the kidney more than 10 times in comparison to animals not treated with vanadium.…”

Copper, Zinc and Magnesium in Non-Insulin-Dependent Diabetes Mellitus Treated with Metformin

“…[42] There are some clinical data showing that zinc deficiency increased occurrence of cataract among people with diabetes mellitus. [17] Zinc and magnesium concentrations decreased in experimentally induced diabetes in rats [43].…”