Evaluation of Toxicity and Anti-Oxidation Activity of the Extracts from Halymenia durvillei

Chaiwichien, Athit; Samrit, Tepparit; Osotprasit, Supawadee; Kueakhai, Pornanan; Sobhon, Prasert; Meemon, Krai; Niamnont, Nakorn; Manohong, Preeyanuch; Pranweerapaiboon, Kanta; Tamtin, Montakan; Changklungmoa, Narin

doi:10.48048/tis.2022.3032

Cited by 3 publications

(6 citation statements)

References 32 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results implied that HDHE inhibited proliferation of A172 cells at concentrations lower than IC50. HDHE concentration at IC50 level tested in A172 cells has less toxic to normal mammalian cells like mouse macrophage, keratinocyte and hepatocyte as previously reported [6]. Thus, cytotoxic concentration of HDHE may appropriate to inhibit proliferation of A172 cells, while safe to normal cells.…”

Section: Cytotoxicity and Proliferation Effectssupporting

confidence: 62%

“…HD extracts might be beneficial to applied as disease-preventing substances as well as anticancer agents due to the enrichment of bioactive ingredients. The hexane solvent extract from HD showed high antioxidant activity tested by DPPH and ABTS assays [6]. The extract, which enriched with n-hexadecanoic acid (palmitic acid) could inhibited proliferation against MDA-MB-231 triple-negative breast cancer cells by induction of apoptotic and autophagic cell death and associated with endoplasmic reticulum stress pathway [7].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Apoptotic and Autophagic Cell Death Effects of the Hexane Extract of Tropical Marine Algae Halymenia durvillei against Human Glioblastoma Cells: In vitro and in silico Studies

Vivithanaporn,

Siangcham,

Tanawoot

et al. 2023

Trends Sci

View full text Add to dashboard Cite

Glioblastoma (GBM) considered as aggressive brain cancer with high mortality rate in patients even after surgical resection. Resistant to chemotherapy is the major problem in GBM therapy. Discovery of novel bioactive compounds from algae is being investigated as alternative sources for potential treatment as well as prevention in glioblastoma. This study revealed the effects of marine red algae extract from hexane solvent fraction of Halymenia durvillei (HDHE) on proliferation and cell death in A172 human GBM cells. HDHE decreased proliferation and promoted cell cycle arrest at G2/M phase. HDHE induced apoptotic cell death in A172 cells through mitochondrial membrane dysfunction, the decrease of anti-apoptotic Bcl-2 protein expression, and activation of caspase 3/7. Moreover, HDHE increased intracellular reactive oxygen species (ROS) production and accumulation of LC3-II, an autophagic marker. The docked conformation of palmitic acid, a major component of HDHE, showed a high affinity binding to TP53 and Beclin-1 as cell death-related target molecules. This research conclusively demonstrated that HDHE might serve as a potent anticancer agent against glioblastoma by promoting apoptotic and autophagic cell death in A172 human GBM cells. HIGHLIGHTS The palmitic acid-enriched extract of red alga Halymenia durvillei (HDHE) could inhibit proliferation of A172 human glioblastoma cells by arresting cell cycle at G2/M phase HDHE could induce both caspase-dependent apoptotic and autophagic death of A172 cells Palmitic acid, a major component of HDHE, showed a high affinity binding to TP53 and Beclin-1 The extract could be beneficial to develop as chemopreventive agents or food supplements against glioblastoma GRAPHICAL ABSTRACT

show abstract

Section: Cytotoxicity and Proliferation Effectssupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Apoptotic and Autophagic Cell Death Effects of the Hexane Extract of Tropical Marine Algae Halymenia durvillei against Human Glioblastoma Cells: In vitro and in silico Studies

Vivithanaporn,

Siangcham,

Tanawoot

et al. 2023

Trends Sci

View full text Add to dashboard Cite

show abstract

“…The present study examined the anticancer properties of marine red algae, H. durvillei. Previous studies revealed the biological effects of extracts from H. durvillei extracts, including antibacterial (Kasmiati et al, 2022), antioxidant (Chaiwichien et al, 2022), antidiabetic, and anti-wrinkling (Arguelles, 2022). Recently, we successfully identified the chemical compositions of HDEA.…”

Section: Discussionmentioning

confidence: 99%

“…HDEA was prepared as previously described (Manohong et al, 2021;Chaiwichien et al, 2022). Briefly, the dried 1.1 kg of H. durvillei was milled and macerated with 5 L of 95% ethanol for one week, and the solvent was evaporated using a rotary evaporator.…”

Section: Extraction and Isolationmentioning

confidence: 99%

“…Previous studies have revealed the biological effects of H. durvillei extracts, including antibacterial (Kasmiati et al, 2022), antioxidant, antidiabetic, and anti-wrinkling (Arguelles, 2022). Recently, we successfully identified the chemical compositions of H. durvillei in ethyl acetate (HDEA) fraction and determined the antioxidant and anti-lung cancer properties of this extract (Manohong et al, 2021;Chaiwichien et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ethyl Acetate Extract of Halymenia durvillei Induced Apoptosis, Autophagy, and Cell Cycle Arrest in Colorectal Cancer Cells

Chantree

Martviset

Sornchuer

et al. 2023

Prev. Nutr. Food Sci.

Self Cite

View full text Add to dashboard Cite

Colorectal cancer is one of the most death-dealing cancers. However, conventional cancer treatments still have side effects. Therefore, novel chemotherapeutic agents with less side effects are still in search. A marine red seaweed, Halymenia durvillei, is recently interested in its anticancer effects. This study investigated the anticancer effect of ethyl acetate extract of H. durvillei (HDEA) on HT-29 colorectal cancer cells in association with the phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway. HDEA-treated HT-29 and OUMS-36 cells were used for cell viability tests by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide assay. The effects of HDEA on apoptosis and cell cycle were evaluated. The nuclear morphology and mitochondrial membrane potential (m) were observed by Hoechst 33342 and JC-1 staining, respectively. The gene expression of PI3K, AKT, and mTOR genes was evaluated using a real-time semiquantitative reverse transcription-polymerase chain reaction. The corresponding protein expressions were assessed by western blot analysis. The result revealed that the cell viability of treated HT-29 cells diminished while that of OUMS-36 cells was non-significant. By the down-regulation of cyclin-dependent kinase 4 and cyclin D1, HDEA-treated HT-29 cells were arrested in the G0/G1 phase. By the up-regulation of cleaved poly(adenosine diphosphate-ribose) polymerase, caspase-9, caspase-8, caspase-3, and Bax, HDEA-treated HT-29 cells underwent apoptosis, but suppressed Bcl-2, disrupted nuclear morphology and m. Furthermore, treated HT-29 cells underwent autophagy by up-regulation of light chain 3-II and beclin-1. Lastly, HDEA suppressed the expression of PI3K, AKT, and mTOR. Therefore, HDEA exerts anticancer effects against HT-29 cells, confirmed by apoptosis, autophagy, and cell cycle arrest induction via regulation of the PI3K/AKT/mTOR signaling pathway.

show abstract

The Scavenging Activity and Safety Effect of Red Marine Algae Acanthophora spicifera Ethanol Extract

Samrit,

Osodprasit,

Chaiwichien

et al. 2023

Trends Sci

View full text Add to dashboard Cite

The red algae, Acanthophora spicifera, are major species grown in Asia and Pacific areas, especially along the coast of southern Thailand. They have the fast growth rate that leads to an abundant supply; thus, they are currently used for wastewater treatment. Furthermore, an ethanolic extract from A. spicifera has been used as a food supplement because of its perceived antioxidant property. Therefore, this study aims to ascertain and evaluate the antioxidant activity and safety of the ethanolic extract from A. spicifera. Safety tests showed that the extract did not have cytotoxicity and genotoxicity effects at concentrations less than 100 µg/mL. In term of antioxidant activity, the DPPH and ABTS assays showed a Trolox equivalent antioxidant capacity (TEAC) of 5.24±0.44 and 4.03±0.13 mg Trolox/g of the extract, respectively. In conclusion, A. spicifera extract is relatively safe and has a rather high efficacy in scavenging free radicals, which makes it suitable for use as food supplement and cosmetics. However, there are a number of caveats regarding its uses in terms of excessive concentrations and exposure duration to avoid causing cytotoxicity and genotoxicity. HIGHLIGHTS The study evaluates the antioxidant activity of spicifera extract was very strong potential. The ethanol extract of Spicifera is safe in both of cytotoxicity and genotoxicity to cell lines. GRAPHICAL ABSTRACT

show abstract

Evaluation of Toxicity and Anti-Oxidation Activity of the Extracts from Halymenia durvillei

Cited by 3 publications

References 32 publications

Apoptotic and Autophagic Cell Death Effects of the Hexane Extract of Tropical Marine Algae Halymenia durvillei against Human Glioblastoma Cells: In vitro and in silico Studies

Apoptotic and Autophagic Cell Death Effects of the Hexane Extract of Tropical Marine Algae Halymenia durvillei against Human Glioblastoma Cells: In vitro and in silico Studies

Ethyl Acetate Extract of Halymenia durvillei Induced Apoptosis, Autophagy, and Cell Cycle Arrest in Colorectal Cancer Cells

The Scavenging Activity and Safety Effect of Red Marine Algae Acanthophora spicifera Ethanol Extract

Contact Info

Product

Resources

About