Evaluation of tongue inspection and serology for diagnosis of Taenia solium cysticercosis in swine: usefulness of ELISA using purified glycoproteins and recombinant antigen

Sato, Marcello Otake; Yamasaki, Hiroshi; Sako, Yasuhito; Nakao, Minoru; Nakaya, Kazuhiro; Plancarte, A.; Kassuku, A.A.; Dorny, Pierre; Geerts, S.; Benítez‐Ortiz, Washington; Hashiguchi, Yuji; Itō, Akira

doi:10.1016/s0304-4017(02)00383-7

Cited by 46 publications

(34 citation statements)

References 25 publications

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“…There are reports of lower efficiency on Ag research in swine serum even in animals with high infection levels. Part of these studies presented confirmed clinical and post-mortem examination infection (Sciutto et al 1998, Nguekam et al 2003, Rodríguez-Hidalgo et al 2003, Sato et al 2003). Our aim is to implement more accurate and efficient methods through immunoassay evaluation for Ab and Ag detection in order to improve the screening of swine with low levels of parasite burden.…”

supporting

confidence: 55%

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Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden

Gomes

Soares

Bueno

et al. 2007

Mem. Inst. Oswaldo Cruz

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Seven swine were experimentally infected with Taenia solium eggs and blood samples from each animal were periodically collected. At the end of the experiment (t140) the animals did not show clinical aspects of cysticercosis or parasites in tongue inspection. All animals were slaughtered and cut into thin slices in searching for cysts. The number of cysts found in each animal varied from 1 to 85. Enzyme-linked immunosorbent assay (ELISA) tests for antibody (Ab) detection and for antigen (Ag) detection were performed, which presented respectively 71 and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as Ag, five (71%) and six (86%) animals were positive, respectively. The association between Ag-ELISA with any IB (18/14 or LLGP) allowed the detection of all animals at 140 days post-experimental infection (days p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the detection of all animals since 70 days p.e.i., and the association between IB LLGP and Ag-ELISA allowed the detection of all animals since 112 days p.e.i. While all animals could be considered healthy by conventional screening tests, the use of immunoassays for detecting Ab and Ag showed better accuracy; therefore it would be more useful than usual clinical examination for screening cysticercosis in slightly infected pigs.

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supporting

confidence: 55%

“…Sato et al (2003) obtained better sensitivity, as early as 30 days after the experimental infection, in pigs with 16 or more cysts. The use of glycoproteins and recombinant Ag could justify such efficiency.…”

Section: Discussionmentioning

confidence: 98%

Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden

Gomes

Soares

Bueno

et al. 2007

Mem. Inst. Oswaldo Cruz

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“…Other detection methods include highly puriϐied or fractionated parasite glycoproteins (Tsang et al 1991, Aluja et al 1999 or recombinant and synthetic antigens (Sato et al 2003, Handali et al 2004, which increase the sensitivity and the speciϐicity of the tests. The use of DNA recombinant technology permits the obtainment of hi gh amounts of puriϐied proteins, enabling the development of more sensitive and speciϐic assays.…”

Section: Introductionmentioning

confidence: 99%

Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

et al. 2012

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Our objective was to evaluate the diagnosis of swine cysticercosis by examining "ante mortem" (inspection of the tongue), "post mortem" (inspection and detailed necropsy) and ELISA for research in serum of antibodies (Ab-ELISA) and antigens (Ag-ELISA). Seven (7) pigs were experimentally infected orally with eggs of Taenia solium and another 10 were naturally infected. In the pigs experimentally infected, inspection of the tongue was negative in all animals, in the routine inspection detailed necropsy and cysticercis were identified in all of them. In pigs with heavy natural infection, inspection of the tongue identified cysticerci in two (20%), while at inspection with necropsy the parasites were identified in large quantities in all animals. In ELISA for antibody search (Ab-ELISA) TS-14 recombinant protein was used, and in search for antigen (Ag-ELISA) a monoclonal antibody against this protein. In animals experimentally infected, blood was collected weekly for 140 days. The Ab-ELISA identified an increase in titers of antibody to cysticerci 21 days after infection, and at the end of the experimental period six animals (86%) were positive to the test. The search for circulating antigens (Ag-ELISA) was positive in two pigs 28 to 91 days after infection. All naturally infected pigs were positive for Ag-ELISA and Ab-ELISA. The search for antibodies and antigens by ELISA in serum from 30 pigs of a local farm and without history of cysticercosis was negative. Thus, the use of TS-14 antigen in ELISA test (Ab-ELISA) can be useful for the diagnosis of cysticercosis in pigs with low infection.

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“…28 Lingual examination represents a traditional method commonly used by pig traders to detect cysticercosis in living pigs. 29 Although its sensitivity is moderate and many free-ranging pigs have to be screened 30 ,we nevertheless consider lingual examination to be a useful, cost-effective method for identifying highly-infected pigs for T. solium cyst preparation in comparison to the alternative bloodletting and serological confirmation. The number of pigs that have to be tested in order to identify an appropriate animal is not only dependent on the local prevalence of porcine cysticercosis, but also on the availability of detailed information on endemic villages from district veterinarians and local authorities, who are indispensable for a focussed and selective identification process.…”

Section: Discussionmentioning

confidence: 99%

“…Previous reports have indicated a prevalence of porcine cysticercosis in endemic districts between 8.2% and 46.7%. [10][11][12][13] Human and animal disease together cause societal burden and significant economic loss which amounted in the Eastern Cape Province in South Africa to [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] Since T. solium cysticercosis/NCC represents an emerging public health problem in many parts of the world, massive efforts are being made to improve prevention, diagnosis and treatment in humans and swine. 14 For the diagnose of T. solium cysticercosis in humans several serological tests (immunoblot, especially enzyme-linked immunoelectrodiffusion transfer blot [EITB]; antibody-enzyme linked immunosorbent assay [ELISA], antigen-ELISA) are used to confirm clinical and radiological findings.…”

mentioning

confidence: 99%

Taenia solium metacestode preparation in rural areas of sub-Saharan Africa: a source for diagnosis and research on cysticercosis

Schmidt¹,

Sikasunge²,

Odongo-Aginya³

et al. 2015

Afr H. Sci.

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Background: Taenia solium metacestodes/cysts obtained from pig carcasses constitute a primary source for diagnostic tools used for the detection of human cysticercosis. Data on T. solium cyst preparation in Africa is still scarce but required to establish independent reference laboratories. Objectives: The aim of the present study is a) to present the likely yield of T. solium cyst material by the use of two different preparation methods in the field and b) to investigate its suitability for immunodiagnosis of human cysticercosis. Methods: In Zambia, Uganda and Tanzania 670 pigs were screened for T. solium infection. Cysts were prepared by `shaking method´ and 'washing method'. Generated crude antigens were applied in a standard western blot assay. Results: 46 out of 670 pigs (6.9%) were found positive for T. solium (Zambia: 12/367, 3.3%; Uganda: 11/217, 5.1%; Tanzania 23/86, 26.7%). Mean values of 77.7 ml whole cysts, 61.8 ml scolices/membranes and 10.9 ml cyst fluid were obtained per pig. Suitability of collected material for the use as crude antigen and molecular diagnostic techniques was demonstrated.Conclusion: This study clearly shows that T. solium cyst preparation in African settings by simple field methods constitutes an effective way to obtain high quality material as source for diagnostic tools and research purposes.

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Evaluation of tongue inspection and serology for diagnosis of Taenia solium cysticercosis in swine: usefulness of ELISA using purified glycoproteins and recombinant antigen

Cited by 46 publications

References 25 publications

Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden

Comparative evaluation of different immunoassays for the detection of Taenia solium cysticercosis in swine with low parasite burden

Cysticercosis in experimentally and naturally infected pigs: parasitological and immunological diagnosis

Taenia solium metacestode preparation in rural areas of sub-Saharan Africa: a source for diagnosis and research on cysticercosis

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