Evaluation of tissue perfusion in a rat model of hind‐limb muscle ischemia using dynamic contrast‐enhanced magnetic resonance imaging

Luo, Yanping; Mohning, Kurt M.; Hradil, Vincent P.; Wessale, Jerry L.; Segreti, Jason A.; Nuss, Merrill E.; Wegner, Craig D.; Burke, Sandra E.; Cox, Bryan F.

doi:10.1002/jmri.10169

Cited by 36 publications

(30 citation statements)

References 29 publications

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“…For that reason, considerable efforts have been made in recent years to develop robust MRI procedures for the measurement of the local microvascular perfusion of muscle. Two main MRI techniques from which information on perfusion can be obtained should be distinguished: (a) arterial spin labeling (ASL), which makes use of the dynamic magnetic labeling of flowing arterial water to distinguish it from non-flowing tissue water (190)(191)(192); (b) dynamic contrast MRI techniques, which rely on the transient signal change that is caused by the entry of intravenously injected MRI contrast agent in the target tissue (193,194). The detection of the contrast agent can either be based on T 1 shortening with T 1 -weighted MRI (e.g.…”

Section: Mri Of Muscle Perfusion and Oxygenationmentioning

confidence: 99%

Dynamic MRS and MRI of skeletal muscle function and biomechanics

et al. 2006

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MR is a powerful technique for studying the biomechanical and functional properties of skeletal muscle in vivo in health and disease. This review focuses on 31 P, 1 H and 13 C MR spectroscopy for assessment of the dynamics of muscle metabolism and on dynamic 1 H MRI methods for non-invasive measurement of the biomechanical and functional properties of skeletal muscle. The information thus obtained ranges from the microscopic level of the metabolism of the myocyte to the macroscopic level of the contractile function of muscle complexes. The MR technology presented plays a vital role in achieving a better understanding of many basic aspects of muscle function, including the regulation of mitochondrial activity and the intricate interplay between muscle fiber organization and contractile function. In addition, these tools are increasingly being employed to establish novel diagnostic procedures as well as to monitor the effects of therapeutic and lifestyle interventions for muscle disorders that have an increasing impact in modern society.

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Section: Mri Of Muscle Perfusion and Oxygenationmentioning

confidence: 99%

Dynamic MRS and MRI of skeletal muscle function and biomechanics

et al. 2006

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“…While injection techniques constitute the vast majority of methods available to assess tissue blood flow, none of these techniques can adequately track the rapid and massive perfusion changes that occur in activated muscles. With regard to NMR imaging, first-pass Gdchelate injections followed by fast T 1 -weighted acquisitions have been adapted to evaluate muscle blood flow (78)(79)(80). They suffer from the same limitations as the above-mentioned techniques, and in contrast to arterial spin labelling, they add little to existing methods.…”

Section: Nmr Determination Of Skeletal Muscle Perfusion: a Key-role Fmentioning

confidence: 99%

Muscle blood flow and oxygenation measured by NMR imaging and spectroscopy

et al. 2006

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Tissue perfusion and oxygenation in many organs can be evaluated by various NMR techniques. This review focuses on the specificities, limitations and adaptations of the NMR tools available to investigate perfusion and oxygenation in the skeletal muscle of humans and animal models. A description of how they may be used simultaneously is provided as well.1 H NMR spectroscopy of myoglobin (Mb) monitors intramyocytic oxygenation. It measures the level of deoxy-Mb, from which Mb concentration, Mb desaturation/resaturation rates, muscle oxygenation changes and intracellular partial oxygen pressure (pO 2 ) can be calculated. Positive and negative blood oxygen level-dependent (BOLD) contrasts exist in skeletal muscle. BOLD contrasts primarily reflect changes in capillary-venous oxygenation, but are also directly or indirectly dependent on muscle blood volume, perfusion, vascular network architecture and angulation, relative to the main magnetic field. Arterial spin labelling (ASL) techniques, having high spatial and temporal resolution, are the methods of choice to quantify and map skeletal muscle perfusion non-invasively. Limitations of ASL are poor contrast-to-noise ratio and sensitivity to movement; however, with the introduction of specific adaptations, it has been proven possible to measure skeletal muscle perfusion at both rest and during exercise. The possibility of combining these NMR measurements with others into a single dynamic protocol is most interesting. The 'multiparametric functional (mpf) NMR' concept can be extended to include the evaluation of muscle energy metabolism simultaneously with 31 P NMR or with lactate double quantum filtered 1 H NMR spectroscopy, an approach which would make NMR an exceptional tool for non-invasive investigations of integrative physiology and biochemistry in skeletal muscle in vivo.

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“…Injectable fluorescence molecular imaging agents have emerged for detecting key biological processes underlying vascular diseases, including Tl perfusion and targeted imaging of angiogenesis with hybrid single-photon emission computed tomography (SPECT)/computed tomography (CT) imaging in a mouse 1 day after femoral artery occlusion (A). Color-coded SPECT 201 Tl images (green) and 99m Tc-NC100692 images (red) of integrin activation are superimposed on CT images (black and white) of the hind limb. Serial images of 201 Tl perfusion before and after femoral artery occlusion in a pig (B).…”

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confidence: 99%

“…Color-coded SPECT 201 Tl images (green) and 99m Tc-NC100692 images (red) of integrin activation are superimposed on CT images (black and white) of the hind limb. Serial images of 201 Tl perfusion before and after femoral artery occlusion in a pig (B). Decreased perfusion is seen in the ischemic limb, which slowly improves over the 4 weeks after occlusion.…”

mentioning

confidence: 99%