Evaluation of time passed since death by examination of oxidative stress markers, histopathological, and molecular changes of major organs in male albino rats

Welson, Nermeen N.; Batiha, Gaber El‐Saber; Ahmed, Sabreen Mahmoud

doi:10.1007/s00414-020-02463-1

Cited by 10 publications

(7 citation statements)

References 17 publications

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“…During the study of cytograms of the heart of cadavers of animals of both experimental groups, no destructive changes in cells were registered during the experimental period. The obtained data on the cytomorphology of the heart confirm the results of the studies of Welson et al (2021), who prove that the processes of fragmentation and vacuolization of cytoplasm in rat cardiomyocytes begin no earlier than 96 h after the death of the animals.…”

Section: Resultssupporting

confidence: 86%

Cytomorphological characteristics of necroptates of internal organs of dogs in the early post-mortem period in the aspect of forensic veterinary examination

Yatsenko¹,

Kazantsev²

2022

Ukrainian journal of veterinary sciences

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The relevance of the study lies in the need for forensic veterinary examination of animal corpses for scientific justification of informative diagnostic criteria for assessing the prescription of death, especially in the early post-mortem period. However, information about early post-mortem changes in dog cadavers at the microstructural level in the Ukrainian and foreign scientific literature is quite fragmentary. In this regard, the purpose of this paper is to establish the informative dynamics of the processes of cell destruction and bacterial contamination of internal organs of dog corpses during the first post-mortem day to establish probable expert criteria for the prescription of death of sub-expert animals during the forensic veterinary examination. A leading approach to the investigation of this problem is the method of obtaining a series of necroptates from lungs, heart, liver, spleen, kidney, pancreas, and brain from canine cadavers, over the same time interval during the first day after death. In cytological preparations obtained from necroptates, the number of destroyed cells and bacterial units was counted using optical microscopy. Based on the results of the dynamics of bacterial contamination and the intensity of morphological changes in spleen and pancreatic cells, their expert information content was established to solve the question of the prescription of death of dogs, regardless of weight and fatness indicators. It was found that the dynamics of bacterial contamination and cellular destruction of the brain, kidneys, and lungs of dog corpses have average expert information content, while the liver and heart are not informative. It was proved that the dynamics of destructive post-mortem processes in the cells of the compact organs of the corpses of dogs of different weight and fatness at the appropriate times probably do not differ and develop with the same intensity. The obtained results of the study will have significance both in the theory of forensic veterinary examination and directly applied, specifically when the forensic expert solves the question regarding the time limit for the death of the animal

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Section: Resultssupporting

confidence: 86%

Cytomorphological characteristics of necroptates of internal organs of dogs in the early post-mortem period in the aspect of forensic veterinary examination

Yatsenko¹,

Kazantsev²

2022

Ukrainian journal of veterinary sciences

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show abstract

“…During the study of cytograms of the heart of cadavers of animals of both experimental groups, no destructive changes in cells were registered during the experimental period. The obtained data on the cytomorphology of the heart confirm the results of the studies of Welson et al (2021), who prove that the processes of fragmentation and vacuolization of the cytoplasm in rat cardiomyocytes begin no earlier than 96 h after the death of the animals.…”

Section: Resultssupporting

confidence: 86%

Untitled

2022

Ukrainian journal of veterinary sciences

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“…The following animals were used in the studies reviewed: pigs (36.8% of animal studies), rats (36.8%), mice (21%), fish (5.2%) and cattle (5.2%). The number of cases included a minimum of 2 and a maximum of 90 animals per study [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ].…”

Section: Resultsmentioning

confidence: 99%

Systematic Review on Post-Mortem Protein Alterations: Analysis of Experimental Models and Evaluation of Potential Biomarkers of Time of Death

et al. 2022

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Estimating the post-mortem interval (PMI) is a very complex issue due to numerous variables that may affect the calculation. Several authors have investigated the quantitative and qualitative variations of protein expression on post-mortem biological samples in certain time intervals, both in animals and in humans. However, the literature data are very numerous and often inhomogeneous, with different models, tissues and proteins evaluated, such that the practical application of these methods is limited to date. The aim of this paper was to offer an organic view of the state of the art about post-mortem protein alterations for the calculation of PMI through the analysis of the various experimental models proposed. The purpose was to investigate the validity of some proteins as “molecular clocks” candidates, focusing on the evidence obtained in the early, intermediate and late post-mortem interval. This study demonstrates how the study of post-mortem protein alterations may be useful for estimating the PMI, although there are still technical limits, especially in the experimental models performed on humans. We suggest a protocol to homogenize the study of future experimental models, with a view to the next concrete application of these methods also at the crime scene.

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Evaluation of time passed since death by examination of oxidative stress markers, histopathological, and molecular changes of major organs in male albino rats

Cited by 10 publications

References 17 publications

Cytomorphological characteristics of necroptates of internal organs of dogs in the early post-mortem period in the aspect of forensic veterinary examination

Cytomorphological characteristics of necroptates of internal organs of dogs in the early post-mortem period in the aspect of forensic veterinary examination

Untitled

Systematic Review on Post-Mortem Protein Alterations: Analysis of Experimental Models and Evaluation of Potential Biomarkers of Time of Death

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