Evaluation of three serological tests for brucellosis in naturally infected cattle using latent class analysis

Muma, John Bwalya; Toft, Nils; Oloya, James; Lund, Arve; Nielsen, Klaus; Samui, K.L.; Skjerve, Eystein

doi:10.1016/j.vetmic.2007.05.012

Cited by 48 publications

(29 citation statements)

References 19 publications

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“…Using the procedure of (Dohoo et al, 2003), a sample size of 76 herds was calculated from the study areas by assuming that brucellosis existed at 17% (Muma et al, 2006). The sample sizes of individual animals per farm were estimated using Herdacc® (Jordan, 1995), by assuming that the sensitivity (Se) and specificity (Sp) for RBT were 90% and 75% (Muma et al, 2007c), and the c-ELISA, Se and Sp 98% and 99%, respectively (Nielsen et al, 1996). Based on the above assumptions, herd sensitivity (Hse) and herd specificity (Hsp) were estimated at 98.0% and 100%, respectively, when a 10% sampling fraction was used and farms classified as positive when at least a single brucellosis positive animal was detected.…”

Section: Study Sites and Designmentioning

confidence: 99%

A comparative study of the seroprevalence of brucellosis in commercial and small-scale mixed dairy–beef cattle enterprises of Lusaka province and Chibombo district, Zambia

Chimana

Muma

Samui

et al. 2010

Trop Anim Health Prod

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Section: Study Sites and Designmentioning

confidence: 99%

A comparative study of the seroprevalence of brucellosis in commercial and small-scale mixed dairy–beef cattle enterprises of Lusaka province and Chibombo district, Zambia

Chimana

Muma

Samui

et al. 2010

Trop Anim Health Prod

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“…In livestock, the clinical diagnosis of brucellosis is based mainly on the occurrence of abortion and orchitis and, in more chronic cases, on infertility and joint hygromas (Acha and Szyfres, 2003). Numerous serological tools are available for the confirmation of a presumptive diagnosis of brucellosis (Nielsen, 2002;Konstantinidis et al, 2007;Muma et al, 2007). Although several biochemical, antigenic and molecular tools have been developed to type Brucella isolates (Le Fleche et al, 2006;Fretin et al, 2008), they all require sophisticated equipment and highlyqualified personnel.…”

Section: Diagnosismentioning

confidence: 99%

Zoonotic tuberculosis and brucellosis in Africa: neglected zoonoses or minor public-health issues? The outcomes of a multi-disciplinary workshop

Marcotty

Matthys

Godfroid

et al. 2009

Annals of Tropical Medicine & Parasitology

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“…On the other hand, Ahmed et al (2011) reported higher sensitivity (100%) and specificity (94.92%) of RBT. Additionally, higher sensitivity and lower specificity of RBT were also obtained by Muma et al (2007).…”

Section: Discussionmentioning

confidence: 95%

Seroprevalence of brucellosis in cattle in selected area of Bangladesh and comparison between Rose Bengal test and i-ELISA used for the screening of brucellosis

Rahman¹,

Chakrabartty²,

Islam³

et al. 2012

Korean Journal of Veterinary Service

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Brucellosis, a bacterial zoonoses caused by the genus Brucella is responsible for abortion and infertility in cow. Brucellosis is causing economic loss in dairy industries and prevalent worldwide including Bangladesh but limited studies are devoted to determine the prevalence and its association with reproductive factors of dairy cows in Bangladesh. Therefore, the present study was conducted to determine the seroprevalence of brucellosis in dairy cattle using screening test Rose Bengal test (RBT) and the positive sera were further confirmed by indirect-ELISA. For this purpose, a total of 400 serum samples from dairy cows with history of abortion and various reproductive disorders were collected from the Kurigram district of Bangladesh for the detection of Brucella antibody. The overall prevalence of brucellosis in dairy cattle was 2.25%. Brucellosis in cases of abortion and repeat breeding was 8.3% and 2.8%, respectively. The results shows higher prevalence of brucellosis in cases of abortion followed by repeat breeding, while there was no seropositive cases from other reproductive disorders. Age-wise seroprevalence was found 3.0% in 2∼3 years age group and 2.0% in 4∼8 years age group. The prevalence of brucellosis in indigenous and cross-bred cattle was 3.6% and 1.7%, respectively. All the animals detected positive to brucellosis by RBT were not found to be positive by i-ELISA. However, the RBT might be a suitable screening test for the diagnosis of Brucella infection in field condition in Bangladesh. These data will help to develop effective disease prevention strategies.

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Evaluation of three serological tests for brucellosis in naturally infected cattle using latent class analysis

Cited by 48 publications

References 19 publications

A comparative study of the seroprevalence of brucellosis in commercial and small-scale mixed dairy–beef cattle enterprises of Lusaka province and Chibombo district, Zambia

A comparative study of the seroprevalence of brucellosis in commercial and small-scale mixed dairy–beef cattle enterprises of Lusaka province and Chibombo district, Zambia

Zoonotic tuberculosis and brucellosis in Africa: neglected zoonoses or minor public-health issues? The outcomes of a multi-disciplinary workshop

Seroprevalence of brucellosis in cattle in selected area of Bangladesh and comparison between Rose Bengal test and i-ELISA used for the screening of brucellosis

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