Evaluation of three recombinant outer membrane proteins, OmpA1, Tdr, and TbpA, as potential vaccine antigens against virulent Aeromonas hydrophila infection in channel catfish (Ictalurus punctatus)

Abdelhamed, Hossam; Ibrahim, Iman; Nho, Seong Won; Banes, Michelle M.; Wills, Robert W.; Karsi, Attila; Lawrence, Mark L.

doi:10.1016/j.fsi.2017.05.043

Cited by 53 publications

(33 citation statements)

References 56 publications

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“…While we did not confirm liver pathology in the AS-challenged trout, past studies in other fish species have provided evidence for liver pathology post-infection with another Aeromonas species ( A. hydrophila) at similar timescales (e.g. for catfish: [ 31 ]; for Golden Mahseer: [ 32 ]). In this study, to validate that a systematic immune response occurred to AS challenge, we used quantitative PCR gene expression profiling to demonstrate a strong transcriptional up-regulation of the pro-inflammatory cytokines IL-1β and TNF-α2 in head kidney (i.e.…”

Section: Resultscontrasting

confidence: 89%

High-throughput proteomic profiling of the fish liver following bacterial infection

et al. 2018

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BackgroundHigh-throughput proteomics was used to determine the role of the fish liver in defense responses to bacterial infection. This was done using a rainbow trout (Oncorhynchus mykiss) model following infection with Aeromonas salmonicida, the causative agent of furunculosis. The vertebrate liver has multifaceted functions in innate immunity, metabolism, and growth; we hypothesize this tissue serves a dual role in supporting host defense in parallel to metabolic adjustments that promote effective immune function. While past studies have reported mRNA responses to A. salmonicida in salmonids, the impact of bacterial infection on the liver proteome remains uncharacterized in fish.ResultsRainbow trout were injected with A. salmonicida or PBS (control) and liver extracted 48 h later for analysis on a hybrid quadrupole-Orbitrap mass spectrometer. A label-free method was used for protein abundance profiling, which revealed a strong innate immune response along with evidence to support parallel rewiring of metabolic and growth systems. 3076 proteins were initially identified against all proteins (n = 71,293 RefSeq proteins) annotated in a single high-quality rainbow trout reference genome, of which 2433 were maintained for analysis post-quality filtering. Among the 2433 proteins, 109 showed significant differential abundance following A. salmonicida challenge, including many upregulated complement system and acute phase response proteins, in addition to molecules with putative functions that may support metabolic re-adjustments. We also identified novel expansions in the complement system due to gene and whole genome duplication events in salmonid evolutionary history, including eight C3 proteins showing differential changes in abundance.ConclusionsThis study provides the first high-throughput proteomic examination of the fish liver in response to bacterial challenge, revealing novel markers for the host defense response, and evidence of metabolic remodeling in conjunction with activation of innate immunity.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5092-0) contains supplementary material, which is available to authorized users.

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Section: Resultscontrasting

confidence: 89%

High-throughput proteomic profiling of the fish liver following bacterial infection

et al. 2018

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“…Cell surface proteins such as outer membrane protein (OMP) is commonly used as vaccine in aquaculture. OMP (A. hydrophila, V. harveyi, Stenotrophomonas maltophilia & V.alginolyticus) were tested in aquaculture animals such as rohu (Yadav et al, 2018) , channel catfish (Abdelhamed et al, 2017;Wang et al, 2016) , goldfish (Carassius auratus) (Divya et al, 2015) , turbot (Scophthalmus maximus) (Wang et al, 2011) and tiger shrimp (Maftuch et al, 2013) showed stimulation towards immune responses (up-regulation of immune-related genes, non-specific parameters and specific antibodies) , reduced bacterial load and mortality. Recombinant OMPs (Flavobacterium columnare, V. anguillarum, Piscirickettisia salmonis & A. hydrophila) were successfully synthesized and tested in grass carp (Luo et al, 2016) , rohu (Hamod et al, 2012) , coho salmon (Oncorhynchus kisutch) (Kuzyk et al, 2001) and common carp (Maiti et al, 2012) .…”

Section: Cell Surface Proteinsmentioning

confidence: 99%

Postbiotics Applications as Infectious Disease Control Agent in Aquaculture

et al. 2020

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Aquaculture is developing so fast that infectious disease outbreak happens regularly. Antibiotic treatment results in development of antibiotic resistance pathogens, thus cause urgent action in searching of other alternative treatment method. Postbiotic was one of the explored strategies among various proposed alternatives. Due to its benefits in agriculture industry, it may be useful in aquaculture industry. Although many reviews were reported on other alternative strategies, the review on postbiotic in aquaculture is limited. This mini review provides an overview of different postbiotics as aquaculture disease control agents. Peptides and exopolysaccharides have antimicrobial properties against bacterial pathogens. Then, short chain fatty acids have both antimicrobial activities against bacterial pathogens and immunostimulating effects to aquatic organism. Vitamins, peptidoglycan and lipopolysaccharide are reported as immunostimulants. Finally, cell surface proteins and teichoic acid can act as vaccine.

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“…Chinese breams ( M. amblycephala ) that were vaccinated with recombinant Omp38 were well‐protected when challenged with A. hydrophila (57.14% RPS) (Wang et al, ). Abdelhamed et al () obtained 98.59%, 95.59% and 47.89% RPS with three recombinant Omps (OmpA1, Tdr, and TbpA) against A. hydrophila infection in channel catfish ( Ictalurus punctatus ). Different protective efficacies of vaccines may be attributed to the immunogenicity of vaccines, the method of immunization, the developmental stage of host, and the doses of challenging bacteria.…”

Section: Discussionmentioning

confidence: 99%

Protective immune responses of recombinant outer membrane proteins OmpF and OmpK of Aeromonas hydrophila in European eel ( Anguilla anguilla )

Zhang

Liao

et al. 2019

Aquac Res

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Outer membrane proteins (Omps) of Gram‐negative bacteria have been proven to be efficient subunit vaccines against bacteriosis. In this study, OmpF and OmpK of Aeromonas hydrophila were expressed, and their immune protective effects in European eel (Anguilla anguilla) were evaluated. The genomic DNA of A. hydrophila 322A was used as a template, and two kinds of prokaryotic expression plasmids, pET‐32a‐OmpF and pET‐32a‐OmpK, were constructed. Recombinant OmpF protein (r‐OmpF) and r‐OmpK were purified and were proven to have antigenicity by Western‐blot analysis. r‐OmpF and r‐OmpK were used as immunogens to immunize European eel by intraperitoneal injection. The mRNA expression of 6 immune‐related genes (IgM, IL‐10, IRF3, IRF7, LysG4 and HexB) in the liver tissues of eels at 1 hr, 3 hr, 6 hr, 12 hr, 24 hr, 72 hr and 10 days postimmunization was analysed by real‐time PCR. At 30 dpi, the serum antibody response was measured by ELISA. Fish were attacked at 15 dpi by live 322A to assess the protective immunity of r‐OmpF and r‐OmpK. All the six tested genes responded to r‐OmpF or r‐OmpK vaccination at varying degrees. The serum antibody titre of r‐OmpF‐ and r‐OmpK‐immunized groups was 1:1,600 and 1:3,200 respectively. In addition, r‐OmpF gave 35.5% of the relative immune protection rate to European eels, while r‐OmpK gave 70.0%. By analysing the protective immunity and the regulatory role in the immune‐related gene expression of the two recombinant proteins that were studied, it was found that r‐OmpK was a potential vaccine candidate against A. hydrophila.

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Evaluation of three recombinant outer membrane proteins, OmpA1, Tdr, and TbpA, as potential vaccine antigens against virulent Aeromonas hydrophila infection in channel catfish (Ictalurus punctatus)

Cited by 53 publications

References 56 publications

High-throughput proteomic profiling of the fish liver following bacterial infection

High-throughput proteomic profiling of the fish liver following bacterial infection

Postbiotics Applications as Infectious Disease Control Agent in Aquaculture

Protective immune responses of recombinant outer membrane proteins OmpF and OmpK of Aeromonas hydrophila in European eel ( Anguilla anguilla )

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