Evaluation of three recombinant Leishmania infantum antigens in human and canine visceral leishmaniasis diagnosis

Fonseca, Aliani Moura; Faria, Angélica Rosa; Rodrigues, Fernandes Tenório Gomes; Nagem, R.A.P.; Magalhães, Rubens Daniel Miserani; Reis-Cunha, João Luís; Bartholomeu, Daniella Castanheira; Andrade, Hélida Monteiro de

doi:10.1016/j.actatropica.2014.04.028

Cited by 33 publications

(13 citation statements)

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“…The “Mix” was therefore a significant improvement as a tool to detect the disease in both humans and dogs, although further optimization would still be required in antigen representation in other to increase sensitivity prior to any considerations regarding a commercial test. Nevertheless, these results contrast with a recent report where a similar mix of three antigens did not lead to an increase in their diagnostic performance when it was compared with the individual proteins alone [ 39 ]. Lack of significant improvement attempted by a mixture of proteins may be explained by the decrease of the antigenicity of each individual protein, due to the presence of the antigenic peptide in lower concentration in the solid phase.…”

Section: Discussioncontrasting

confidence: 99%

“…Several of the antigens tested showed very good potential for the canine form, with one of them (Lci13), demonstrating a better capacity to detect the canine leishmaniasis than the total parasite lysate. This protein is a promising antigen, since some recent studies did not find a single protein capable of such diagnostic efficiency in dogs [ 38 , 39 ]. In contrast, none of the antigens tested were as effective for human VL, highlighting the differences in immune response between the two different disease targets, as highlighted previously [ 23 , 26 ].…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis

et al. 2017

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Current strategies for the control of zoonotic visceral leishmaniasis (VL) rely on its efficient diagnosis in both human and canine hosts. The most promising and cost effective approach is based on serologic assays with recombinant proteins. However, no single antigen has been found so far which can be effectively used to detect the disease in both dogs and humans. In previous works, we identified Leishmania infantum antigens with potential for the serodiagnosis of VL. Here, we aimed to expand the panel of the available antigens for VL diagnosis through another screening of a genomic expression library. Seven different protein-coding gene fragments were identified, five of which encoding proteins which have not been previously studied in Leishmania and rich in repetitive motifs. Poly-histidine tagged polypeptides were generated from six genes and evaluated for their potential for diagnosis of VL by ELISA (Enzyme Linked ImmunoSorbent Assay) with sera from infected humans and dogs. None of those was valid for the detection of human VL (26–52% sensitivity) although their performance was increased in the canine sera (48–91% sensitivity), with one polypeptide useful for the diagnosis of canine leishmaniasis. Next, we assayed a mixture of three antigens, found to be best for human or canine VL, among 13 identified through different screenings. This “Mix” resulted in similar levels of sensitivity for both human (84%) and canine (88%) sera. With improvements, this validates the use of multiple proteins, including antigens identified here, as components of a single system for the diagnosis of both forms of leishmaniasis.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis

et al. 2017

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“…Recently, an increasing number of Leishmania antigens have been evaluated in serodiagnosis [8] , [13] – [16] . High values of sensitivity and specificity are very important to these antigens.…”

Section: Introductionmentioning

confidence: 99%

Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs

et al. 2015

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BackgroundVisceral leishmaniasis is the most severe form of leishmaniasis. Worldwide, approximately 20% of zoonotic human visceral leishmaniasis is caused by Leishmania infantum, also known as Leishmania chagasi in Latin America. Current diagnostic methods are not accurate enough to identify Leishmania-infected animals and may compromise the effectiveness of disease control. Therefore, we aimed to produce and test two recombinant multiepitope proteins as a means to improve and increase accuracy in the diagnosis of canine visceral leishmaniasis (CVL).Methodology/Principal FindingsTen antigenic peptides were identified by CVL ELISA in previous work. In the current proposal, the coding sequences of these ten peptides were assembled into a synthetic gene. Furthermore, other twenty peptides were selected from work by our group where good B and T cell epitopes were mapped. The coding sequences of these peptides were also assembled into a synthetic gene. Both genes have been cloned and expressed in Escherichia coli, producing two multiepitope recombinant proteins, PQ10 and PQ20. These antigens have been used in CVL ELISA and were able to identify asymptomatic dogs (80%) more effectively than EIE-LVC kit, produced by Bio-Manguinhos (0%) and DPP kit (10%). Moreover, our recombinant proteins presented an early detection (before PCR) of infected dogs, with positivities ranging from 23% to 65%, depending on the phase of infection in which sera were acquired.Conclusions/SignificanceOur study shows that ELISA using the multiepitope proteins PQ10 and PQ20 has great potential in early CVL diagnosis. The use of these proteins in other methodologies, such as immunochromatographic tests, could be beneficial mainly for the detection of asymptomatic dogs.

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“…In this sense, for this work a recombinant protein with unknown function in L. infantum (hypothetical C1 protein or C1 antigen), which showed reactive peptides against canine sera infected by L. infantum and was not reactive to sera infected with Trypanosoma cruzi (Fonseca et al, 2014), was selected as recognition element for the development of an surface plasmon resonance (SPR) immunosensor. The SPR technique was chosen for this study because it permits the evaluation of the antigen-antibody interaction in real time and without the need of chemical or biological markers.…”

Section: Introductionmentioning

confidence: 99%

SPR analysis of the interaction between a recombinant protein of unknown function in Leishmania infantum immobilised on dendrimers and antibodies of the visceral leishmaniasis: A potential use in immunodiagnosis

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et al. 2015

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Evaluation of three recombinant Leishmania infantum antigens in human and canine visceral leishmaniasis diagnosis

Cited by 33 publications

References 32 publications

Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis

Evaluation of a new set of recombinant antigens for the serological diagnosis of human and canine visceral leishmaniasis

Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs

SPR analysis of the interaction between a recombinant protein of unknown function in Leishmania infantum immobilised on dendrimers and antibodies of the visceral leishmaniasis: A potential use in immunodiagnosis

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