Evaluation of Three Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

Chapuy‐Regaud, Sabine; Miédougé, Marcel; Abravanel, Florence; Silva, Isabelle Da; Porcheron, Marion; Fillaux, Judith; Dimeglio, Chloé; Izopet, Jacques

doi:10.1128/spectrum.01376-21

Cited by 25 publications

(23 citation statements)

References 36 publications

(42 reference statements)

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“…Whilst a nasopharyngeal swab (NPS) positivity [1,8] and seropositivity [4,12,18] similar to the present study was already reported elsewhere, some studies [15,16,36] found a higher risk of seropositivity for males or females [19]. In contrast with some studies [8,30,32,37] we found no association between age and risk of SARS-CoV-2 infection.…”

Section: Interpretation Of Findings In Relation To Other Studiessupporting

confidence: 85%

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Occupational Risk Factors for SARS-CoV-2 Infection in Hospital Health Care Workers: A Prospective Nested Case-Control Study

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Negro

D’Agaro

et al. 2022

Life

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Introduction: Health Care Workers (HCWs) are at a particular high risk of SARS-CoV-2 infection due to direct and indirect exposure to COVID-19 patients and Aerosol-Generating Procedures (AGPs). The aim of the study was to assess the risk factors for SARS-CoV-2 infection in HCWs exposed to COVID-19 patients, to evaluate the adherence and effectiveness of Infection Prevention and Control (IPC) measures, to describe the clinical presentation for SARS-CoV-2 infection in HCWs and to determine serological responses in HCWs. Methods: HCWs exposed to COVID-19 patients during the previous 14 days with a confirmed case status were recruited as cases; HCWs exposed to COVID-19 patients during the previous 14 days in the same ward without a suspected/probable/confirmed case status were recruited as controls. Serum samples were collected as soon as possible and after 21–28 days from all participants. Data were collected with a WHO standardized questionnaire as soon as possible and after 21–28 days. Results: All social, occupational and personal variables considered were not associated with an increased risk of SARS-CoV-2 infection. Conclusions: Our study showed a high knowledge of IPC measures and very high PPE use among HCWs.

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Section: Interpretation Of Findings In Relation To Other Studiessupporting

confidence: 85%

“…Sera were additionally tested with ELISA assay Wantai SARS-CoV-2 Ab ELISA (Beijing Wantai Biological Pharmacy Enterprise). Samples with a cutoff ratio (absorbance of the sample at 450 nm divided by 0.19) ≥1 AU/mL were considered positive [36].…”

Section: Methodsmentioning

confidence: 99%

Occupational Risk Factors for SARS-CoV-2 Infection in Hospital Health Care Workers: A Prospective Nested Case-Control Study

Dusefante

Negro

D’Agaro

et al. 2022

Life

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“…SARS-CoV-2 IgG II Quant immunoassay detects all IgG antibodies against SARS-CoV-2, which includes neutralizing antibodies against the receptor binding domain (RBD) of the SARS-CoV-2 spike (S) protein, but are not exclusive to it. Chapuy-Regaud et al also report a moderate correlation (Spearman’s r = 0.63) between the results of SARS-CoV-2 IgG Quant immunoassay and live virus neutralization assays in known positive samples [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

Neutralizing Antibody Response to BBIBP-CorV in Comparison with COVID-19 Recovered, Unvaccinated Individuals in a Sample of the Pakistani Population

et al. 2022

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Fifty five percent of the Pakistani population is still unvaccinated with the two-dose protocol of COVID-19 vaccines. This study was undertaken to determine the seroconversion rate and antibody titers following the two-dose BBIBP-CorV protocol, and to compare these variables in unvaccinated, COVID-19 recovered individuals (total n = 180) at Indus Hospital and Health Network, Karachi. Pseudotyped lentivirus antibody neutralization assays and SARS-CoV-2 IgG Quant II (Abbott) immunoassays were performed 4-8 weeks following the second dose of the BBIBP-CorV or PCR positivity/onset of symptoms of COVID-19. Seroconversion rate, using neutralization assays, in vaccinated individuals was lower (78%) than that in unvaccinated, COVID-19-recovered individuals with moderate to severe infection (97%). Prior PCR positivity increased serocoversion rate to 98% in vaccinated individuals. Immunoassays did not, however, reveal significant inter-group differences in seroconversion rates (≥95% in all groups). Log10 mean antibody neutralizing titers following the two-dose BBIBP-CorV protocol (IC50 = 2.21) were found to be significantly less than those succeeding moderate to severe COVID-19 (IC50 = 2.94). Prior SARS-CoV-2 positivity significantly increased post-vaccination antibody titers (IC50 = 2.82). Similar inter-group titer differences were obtained using the immunoassay. BBIBP-CorV post-vaccination titers may, thus, be lower than those following natural, moderate to severe infection, while prior SARS-CoV-2 exposure increases these titers to more closely approximate the latter.

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“…We measured the antibody titers in 1,169 vaccinated individuals shortly before their breakthrough infection with the Omicron SARS-CoV-2 variant (1 November -31 December, 2021). Total SARS-CoV-2 antibodies were measured with a quantitative enzyme-linked immunosorbent assay (ELISA) (Wantai Biological Pharmacy Enterprise Co., Ltd, China) [5].…”

Section: Dear Editormentioning

confidence: 99%

Antibody titers and breakthrough infections with Omicron SARS-CoV-2

Migueres¹,

Mansuy²,

Saivin³

et al. 2022

Journal of Infection

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Evaluation of Three Quantitative Anti-SARS-CoV-2 Antibody Immunoassays

Abstract: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in December 2019 and caused a dramatic pandemic. Serological assays are used to check for immunization and assess herd immunity.

Cited by 25 publications

References 36 publications

Occupational Risk Factors for SARS-CoV-2 Infection in Hospital Health Care Workers: A Prospective Nested Case-Control Study

Occupational Risk Factors for SARS-CoV-2 Infection in Hospital Health Care Workers: A Prospective Nested Case-Control Study

Neutralizing Antibody Response to BBIBP-CorV in Comparison with COVID-19 Recovered, Unvaccinated Individuals in a Sample of the Pakistani Population

Antibody titers and breakthrough infections with Omicron SARS-CoV-2

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