Evaluation of three Bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing Vibriosis and Aeromonosis

Sam-on, Muhamad Firdaus Syahmi; Mustafa, Shuhaimi; Yusof, Mohd Termizi; Hashim, Amalia Mohd; Abbasiliasi, Sahar; Zulkifly, Shahrizim; Jahari, Muhamad Amin; Roslan, Muhamad Akhmal Hakim

doi:10.1016/j.micpath.2022.105417

Cited by 14 publications

(8 citation statements)

References 52 publications

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“…The co-aggregation rates of B. HLJ1 and B. C1 with V. parahaemolyticus were 43.4% and 36.7% after the first 2 h, while they were 65.6% and 74.3% at 24 h, respectively ( Figure 4 B). The results were comparable to those previously reported, such as the co-aggregation rate of B. subtilis with Aeromonas hydrophila being 80% after 24 h [ 33 ], indicating the great potential of B. HLJ1 and B. C1 to control V. parahaemolyticus infection in aquaculture animals.…”

Section: Resultssupporting

confidence: 89%

“…The safety of probiotics is very important for aquaculture; therefore, safety tests including antibiotic resistance and virulence genes need to be conducted on candidate probiotics before application. Antibiotic resistance genes can be transferred between bacteria, leading to the development of antibiotic-resistant bacteria [ 33 ]. Thus, antibiotic resistance of probiotic bacteria is a key factor for application in aquaculture.…”

Section: Resultsmentioning

confidence: 99%

“…Auto-aggregation and co-aggregation assays were described by Mustafa et al [ 33 ]. The isolated strains were incubated in LB medium for 24 h at 37 °C and centrifuged at 5000 rpm for 10 min.…”

Section: Methodsmentioning

confidence: 99%

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Isolation of Bacillus subtilis and Bacillus pumilus with Anti-Vibrio parahaemolyticus Activity and Identification of the Anti-Vibrio parahaemolyticus Substance

et al. 2023

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The adoption of intensive farming has exacerbated disease outbreaks in aquaculture, particularly vibriosis caused by Vibrio parahaemolyticus. The use of probiotics to control V. parahaemolyticus is recognized as a good alternative to antibiotics for avoiding the development of antibiotic-resistant bacteria. In this study, two strains of B. HLJ1 and B. C1 with strong inhibitory activity on V. parahaemolyticus were isolated from aquaculture water and identified as Bacillus subtilis and Bacillus pumilus, respectively. Both B. HLJ1 and B. C1 lacked antibiotic resistance and virulence genes, suggesting that they are safe for use in aquaculture. In addition, these two strains can tolerate acid environments, produce spores, secrete extracellular enzymes, and co-aggregate as well as auto-aggregate with V. parahaemolyticus. B. HLJ1 and B. C1 produced the same anti-V. parahaemolyticus substance, which was identified as AI-77-F and belongs to amicoumacins. Both B. C1 and B. HLJ1 showed inhibitory activity against 11 different V. parahaemolyticus and could effectively control the growth of V. parahaemolyticus in simulated aquaculture wastewater when the concentration of B. C1 and B. HLJ1 reached 1 × 107 CFU/mL. This study shows that B. HLJ1 and B. C1 have great potential as aquaculture probiotics.

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Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

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Isolation of Bacillus subtilis and Bacillus pumilus with Anti-Vibrio parahaemolyticus Activity and Identification of the Anti-Vibrio parahaemolyticus Substance

et al. 2023

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“…A recent study demonstrated that the increase in Pediococcus caused by feeding selenium-enriched Pediococcus acidilactici MRS-7 contributed to intestinal barrier function restoration and alleviation of the inflammatory response and oxidative stress in jejunum-injured mice ( Chen et al, 2022 ). Bacillus strains have been widely used as potential probiotics to combat pathogen invasion and improve growth performance ( Wang et al, 2020 ; Sam-On et al, 2022 ). Zou et al (2022) found that dietary supplementation with Bacillus subtilis decreased proinflammatory cytokine expression and improved antioxidative status and intestinal barrier integrity by regulating the intestinal microenvironment and related microbiota composition in laying hens.…”

Section: Discussionmentioning

confidence: 99%

Modulating gut microbiota and metabolites with dietary fiber oat β-glucan interventions to improve growth performance and intestinal function in weaned rabbits

Luo

Huang

et al. 2022

Front. Microbiol.

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The effect of oat β-glucan on intestinal function and growth performance of weaned rabbits were explored by multi-omics integrative analyses in the present study. New Zealand White rabbits fed oat β-glucan [200 mg/kg body weight (BW)] for 4 weeks, and serum markers, colon histological alterations, colonic microbiome, colonic metabolome, and serum metabolome were measured. The results revealed that oat β-glucan increased BW, average daily gain (ADG), average daily food intake (ADFI), and decreased serum tumor necrosis factor-α (TNF-α) interleukin-1β (IL-1β), and lipopolysaccharide (LPS) contents, but did not affect colonic microstructure. Microbiota community analysis showed oat β-glucan modulated gut microbial composition and structure, increased the abundances of beneficial bacteria Lactobacillus, Prevotellaceae_UCG-001, Pediococcus, Bacillus, etc. Oat β-glucan also increased intestinal propionic acid, valeric acid, and butyric acid concentrations, decreased lysine and aromatic amino acid (AAA) derivative contents. Serum metabolite analysis revealed that oat β-glucan altered host carbohydrate, lipid, and amino acid metabolism. These results suggested that oat β-glucan could inhibit systemic inflammation and protect intestinal function by regulating gut microbiota and related metabolites, which further helps to improve growth performance in weaned rabbits.

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“…The high resistance of Bacillus spp. to 0.3% bile salt and the activities of protease, amylase, and lipase as exhibited by them further certifies their functionality as probiotics of great importance in aquatic environments (Sam-on et al 2022 ). Another study examined different Bacillus strains from mangrove environments as probiotics used in dietary of (Rohu) Labeo rohita .…”

Section: Nanotechnology In Preventing and Remediating Antibiotic Poll...mentioning

confidence: 98%

Antibiotic resistance in aquaculture and aquatic organisms: a review of current nanotechnology applications for sustainable management

Okeke

Chukwudozie

Nyaruaba

et al. 2022

Environ Sci Pollut Res

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Aquaculture has emerged as one of the world’s fastest-growing food industries in recent years, helping food security and boosting global economic status. The indiscriminate disposal of untreated or improperly managed waste and effluents from different sources including production plants, food processing sectors, and healthcare sectors release various contaminants such as bioactive compounds and unmetabolized antibiotics, and antibiotic-resistant organisms into the environment. These emerging contaminants (ECs), especially antibiotics, have the potential to pollute the environment, particularly the aquatic ecosystem due to their widespread use in aquaculture, leading to various toxicological effects on aquatic organisms as well as long-term persistence in the environment. However, various forms of nanotechnology-based technologies are now being explored to assist other remediation technologies to boost productivity, efficiency, and sustainability. In this review, we critically highlighted several ecofriendly nanotechnological methods including nanodrug and vaccine delivery, nanoformulations, and nanosensor for their antimicrobial effects in aquaculture and aquatic organisms, potential public health risks associated with nanoparticles, and their mitigation measures for sustainable management. Graphical abstract

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Evaluation of three Bacillus spp. isolated from the gut of giant freshwater prawn as potential probiotics against pathogens causing Vibriosis and Aeromonosis

Cited by 14 publications

References 52 publications

Isolation of Bacillus subtilis and Bacillus pumilus with Anti-Vibrio parahaemolyticus Activity and Identification of the Anti-Vibrio parahaemolyticus Substance

Isolation of Bacillus subtilis and Bacillus pumilus with Anti-Vibrio parahaemolyticus Activity and Identification of the Anti-Vibrio parahaemolyticus Substance

Modulating gut microbiota and metabolites with dietary fiber oat β-glucan interventions to improve growth performance and intestinal function in weaned rabbits

Antibiotic resistance in aquaculture and aquatic organisms: a review of current nanotechnology applications for sustainable management

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