Evaluation of therapeutic sublingual vaccines in a murine model of chronic house dust mite allergic airway inflammation

Abstract

The efficacy of a sublingual vaccine based on a Dpte/Dfar allergen extract mix was demonstrated in a well standardized murine model of chronic allergic airway inflammation based on clinically relevant mite allergens. The latter will be used as a benchmark for evaluation of future vaccines, including recombinant allergens. This HDM allergic airway inflammation animal model is a useful tool to design and select candidate vaccines to be tested in humans.

Subcutaneous immunotherapy with purified Der p1 and 2 suppresses type 2 immunity in a murine asthma model

“…House dust mite (HDM) is the most prominent source of indoor exposure to allergens and is a cause for allergic rhinitis and asthma 9. The HDM species Dermatophagoides pteronyssinus (Der p) has at least 23 major allergens9 that are thought to contribute to allergic sensitization through their proteolytic activity, activating cells of the innate immune system and priming an adaptive type 2 immune response 2, 9.…”

“…The HDM species Dermatophagoides pteronyssinus (Der p) has at least 23 major allergens9 that are thought to contribute to allergic sensitization through their proteolytic activity, activating cells of the innate immune system and priming an adaptive type 2 immune response 2, 9. In the MAS prospective birth cohort, sensitization patterns for HDM allergens were studied into detail by component‐resolved analysis 10.…”

“…However, HDM extracts are variable in content of allergens,12, 13 and stability is limited 14. Given the fact that sensitization to Der p1 and 2 identifies more than 95% of HDM‐allergic individuals9, 13 and their causal role in early sensitization,10 SIT with purified Der p1 and 2 might be a more attractive therapeutic approach compared to the use of HDM extracts.…”

Intranasal, Liposome-Adjuvanted Cockroach Allergy Vaccines Made of Refined Major Allergen and Whole-Body Extract of <b><i>Periplaneta americana</i></b>

“…mast cells, eosinophils and neutrophils) [17], thereby preventing the release of cellular mediators and late-phase inflammation. Immunotherapy has been shown to be effective for allergies caused by Hymenoptera [18,19], some pollens [20], grasses [21], trees [22] and house dust mites [23]. However, data in the literature are relatively scarce on vaccine efficacy for allergic morbidity, especially asthma, caused by the American CR (ACR; Periplaneta americana ), which is the predominant CR species in Thailand and many other tropical countries.…”

Revisão Sistemática Sobre Modelos Experimentais De Asma Aguda E Crônica Induzidos Com Extrato De Ácaro Da Poeira Doméstica

“…Na presente revisão sistemática foram selecionados cinco artigos de administração crônica. Os resultados mostram uma robusta inflamação e aumento da espessura das paredes das vias aéreas, com deposição de colágeno peribronquial e perivascular, produção de muco, expressão da citocina actina alfa de músculo liso (α-SMA) e hiper-responsividade brônquica à metacolina 15,17,18,22 . Logo, modelos crônicos fornecem desfechos mais próximos à asma clínica com resultados sobre remodelamento das vias aéreas, espessamento da parede, e aumento da produção de muco.…”