Evaluation of Therapeutic Efficacy of Adjuvant <i>Helicobacter pylori</i> Whole Cell Sonicate in Mice with Chronic <i>H. pylori</i> Infection

Maeda, K.; Yamashiro, Tetsu; Minoura, Takanori; Fujioka, Toshio; Nasu, Masaru; Nishizono, Akira

doi:10.1111/j.1348-0421.2002.tb02742.x

Cited by 19 publications

(10 citation statements)

References 21 publications

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“…It is known that Al(OH) 3 is already approved for use as an adjuvant in humans because of its safety and stability. In case of the H. pylori vaccine, systemic immunization with H. pylori antigens in aluminum adjuvants has shown to induce immune protection to a certain degree in different animal models [22,25,26]. Our study also suggested that Mongolian gerbils were partly protected from H. pylori infection by intramuscular administration with H. pylori vaccines comprising different recombinant antigens combined with aluminum adjuvants.…”

Section: Discussionsupporting

confidence: 54%

Protection Against Helicobacter pylori Infection in Mongolian Gerbil by Intragastric or Intramuscular Administration of H. pylori Multicomponent Vaccine

Shi

Guo

et al. 2008

Helicobacter

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Section: Discussionsupporting

confidence: 54%

Protection Against Helicobacter pylori Infection in Mongolian Gerbil by Intragastric or Intramuscular Administration of H. pylori Multicomponent Vaccine

Shi

Guo

et al. 2008

Helicobacter

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“…However, many vaccine studies that focused on inducing Th2 immunity used an H. pylori sonicate preparation that contained H. pylori LPS [14,[19][20][21]. Thus, these studies using H. pylori sonicate may not have optimally induced Th2 biased H. pylori specific immunity.…”

Section: Introductionmentioning

confidence: 99%

Effects of a Th1- versus a Th2-biased immune response in protection against Helicobacter pylori challenge in mice

Taylor

Ziman

Canfield

et al. 2008

Microbial Pathogenesis

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The roles that T helper type 1 (Th1) and T helper type 2 (Th2) H. pylori specific immune responses play in protection from H. pylori challenge are poorly understood. It is expected that Th2 immune responses are required for protection against extracellular bacteria, such as H. pylori. However, recent studies have suggested that Th1 immunity is required for protection. The mechanisms by which this might occur are unknown. Our goal in this study was to more clearly define the effects of a Th1 vs. a Th2 promoting H. pylori vaccine on immunity and protection. Therefore, we tested a Th1 vaccine consisting of an H. pylori sonicate and CpG oligonucleotides (CpG) and a Th2 vaccine consisting of a lipopolysaccharide (LPS) depleted H. pylori sonicate combined with cholera toxin (CT). We demonstrate that although the Th2 promoting vaccine induced stronger systemic and local immune responses, only the Th1 promoting vaccine was protective.

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“…In an attempt to provide an option other than triple therapy, we have studied the effectiveness of preventive or therapeutic vaccinations against H. pylori infection (13,16,22,23), the mechanisms of which remain to be clarified. In the present study, we examined whether DCs pulsed with H. pylori antigens reduced bacterial loads in the stomachs of mice with chronic H. pylori infections and tried to clarify the mechanisms involved to test the feasibility of DC-based immunotherapy against chronic H. pylori infection.…”

mentioning

confidence: 99%

Transfer of Antigen-Pulsed Dendritic Cells Induces Specific T-Cell Proliferation and a Therapeutic Effect against Long-TermHelicobacter pyloriInfection in Mice

Otsu¹,

Gotoh²,

Yamashiro

et al. 2006

Infect Immun

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Helicobacter pylori causes persistent infection of the stomach and results in chronic gastritis and peptic ulcers. Jaws II cells, derived from mouse bone marrow, were pulsed with live or formalin-killed or whole-cell sonicates (WCS) of H. pylori. Representative cell surface molecules were expressed at substantial levels on Jaws II cells, indicating that appropriate maturation of the cells was achieved with the three H. pylori antigens without any significant differences. H. pylori WCS-pulsed Jaws II cells secreted a significant amount of tumor necrosis factor alpha into the culture supernatant. The naïve T cells exposed to the WCS-pulsed Jaws II cells showed significant proliferation and gamma interferon (IFN-␥) and interleukin-10 (IL-10) production in vitro. A 2-log reduction in the number of colonizing bacteria was observed in the mice treated with the WCS-pulsed Jaws II cells; however, no significant reductions were achieved in mice treated with Jaws II cells pulsed with other H. pylori antigens. Up-regulated production of IFN-␥ and IL-10 was observed in the stomachs of the mice treated with the WCS-pulsed Jaws II cells, which is consistent with the result obtained in vitro. There were no differences in gastritis scores or H. pylori-specific antibody titers among the mice treated with Jaws II cells pulsed with the three different H. pylori antigens. The results suggest that Th1 cell-mediated immunity in combination with Th2 cell-mediated immunity plays a role in reducing colonizing bacterial numbers in mice with chronic H. pylori infections.

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Evaluation of Therapeutic Efficacy of Adjuvant Helicobacter pylori Whole Cell Sonicate in Mice with Chronic H. pylori Infection

Cited by 19 publications

References 21 publications

Protection Against Helicobacter pylori Infection in Mongolian Gerbil by Intragastric or Intramuscular Administration of H. pylori Multicomponent Vaccine

Protection Against Helicobacter pylori Infection in Mongolian Gerbil by Intragastric or Intramuscular Administration of H. pylori Multicomponent Vaccine

Effects of a Th1- versus a Th2-biased immune response in protection against Helicobacter pylori challenge in mice

Transfer of Antigen-Pulsed Dendritic Cells Induces Specific T-Cell Proliferation and a Therapeutic Effect against Long-TermHelicobacter pyloriInfection in Mice

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