Evaluation of the wound healing property of Boesenbergia longiflora rhizomes

Sudsai, Teeratad; Wattanapiromsakul, Chatchai; Nakpheng, Titpawan; Tewtrakul, Supinya

doi:10.1016/j.jep.2013.08.038

Cited by 21 publications

(18 citation statements)

References 17 publications

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“…However, excessive NO production acts as a reactive radical, which damages the function of normal cell by attacking normal tissue surrounding the wound site. Thus, the regulation of reactive radical production is an important factor in recruitment of fibroblast, which is attracted into the site to initiate the proliferative phase of healing process [63]. Also, it has been reported that NO directly affect the tumor necrosis factor-alpha (TNF-α) expression, another important mediator in the inflammatory response [62].…”

Section: Resultsmentioning

confidence: 99%

In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

et al. 2019

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Thymus sipyleus Boiss. subsp. rosulans (Borbas) Jalas (TS) is a commonly used plant in the treatment of various complaints, including skin wounds in Turkish folk medicine. Despite the widespread traditional use of TS, there is not any scientific report confirming the effectiveness of this plant on the healing process. This research aimed to investigate the effects of different extracts obtained from TS on biological events during wound healing, on a cellular basis. In this context, proliferative activities of the extracts, as well as the effects on wound closure and hydroxyproline synthesis, were determined. In addition to wound healing properties, the antioxidant, antibacterial and anti-inflammatory activities of the extracts were evaluated. Decoction (D) and infusion (I) extracts contained the highest amount of phenolic content and showed the most potent activity against DPPH radical. All extracts exhibited complete protection against the damage induced by hydrogen peroxide (H2O2) by increasing cell viability compared to only H2O2-treated groups, both in co-treatment and pre-treatment protocols. None of the extracts exhibited cytotoxic activity, and most of the extracts from the TS stimulated fibroblast proliferation and migration. All TS extracts exert anti-inflammatory activity by suppressing the overproduction of tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO). The most pronounced activity on hydroxyproline synthesis was observed in D extract. In summary, it was observed that TS extracts can promote the healing process by enhancing fibroblast migration, proliferation and collagen synthesis as well as suppressing pro-inflammatory cytokines. The obtained data in this work support the traditional use of TS as a valuable plant-based compound for the treatment of wounds.

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Section: Resultsmentioning

confidence: 99%

In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

et al. 2019

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“…Anti-NO production assay. To evaluate the anti-inflammatory activity, the inhibition of nitric oxide (NO) production was evaluated according to a previous report [18]. Briefly, RAW264.7 cells were seeded into sterile 96-well plates (1x10 5 cells/well) and incubated for 1 h at 37˚C in a humidified incubator containing 5% CO 2 .…”

Section: Anti-inflammatory Activitymentioning

confidence: 99%

“…Cell viability test. The cytotoxicity of the test compounds after 24 h of incubation was determined according to a previously reported method [18]. A volume of 10 μl of MTT solution (5 mg/ml in PBS) was added to each well of 96-well plates and further incubated in a CO 2 incubator for 4 h. The formazan products generated by MTT reduction were dissolved in dimethyl sulfoxide (DMSO).…”

Section: Anti-inflammatory Activitymentioning

confidence: 99%

Anti-inflammatory, wound healing and antioxidant potential of compounds from Dioscorea bulbifera L. bulbils

et al. 2020

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Background Dioscorea bulbifera L. (Dioscoreaceae) has been traditionally used in Thai folk medicine as a diuretic and anthelmintic, for longevity preparations, and for wound and inflammation treatment. This plant is also commonly used in traditional Indian and Chinese medicines in the treatment of sore throat, gastric cancer, rectal carcinoma and goiters. However, the wound healing effects of the active compounds in this plant have not been investigated. Objective This study aimed to identify compounds responsible for the wound healing activity of D. bulbifera and determine their potential anti-inflammatory and antioxidant activities. Methods Crude extracts of D. bulbifera bulbils, their derived fractions and eleven purified compounds were tested for anti-inflammatory activity against LPS-induced NO production in RAW264.7 macrophages. The wound healing effects were evaluated via cell proliferation and migration assays using human dermal fibroblasts (HDFs), and the antioxidant effects were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radical (•OH) scavenging activity assays. Results 15,16-Epoxy-6α-O-acetyl-8β-hydroxy-19-nor-clero-13(16),14-diene-17,12;18,2-diolide (2), (+)-catechin (5), quercetin (6) and myricetin (11) exhibited significantly potent wound healing effects and promoted marked cell proliferation, resulting in % viabilities of 107.4–137.6, 121.1–151.9, 98.0–131.9, 90.9–115.9, respectively. Among them, (+)-catechin produced the highest % cell migration, resulting in 100.0% wound closure sooner (at day 2) than the other compounds. In addition, 1 μg/ml (+)-catechin significantly increased fibroblast migration by 2.4-fold compared to that in the control after 24 h. Regarding anti-inflammatory properties, kaempferol (7) and quercetin (6) decreased (p < 0.005) NO production, with IC50 values of 46.6 and 56.2 μM, respectively. In addition, the crude extracts, solvent fractions and flavonoid compounds were also found to possess marked antioxidant activity in both DPPH and •OH radical scavenging assays. Conclusions These findings provide more evidence to support the traditional use of D. bulbifera for the treatment of wounds and inflammation.

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“…To evaluate the anti-inflammatory activity, an inhibitory effect on nitric oxide (NO) production was carried out according to the previous report described by Sudsai et al [14]. Briefly, RAW264.7 cells were seeded onto 96-well plates (1 × 10 5 cells/well) and were maintained to adhere at 37°C for 1 h in a CO 2 incubator containing 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

“…The cytotoxicity of the test compounds after 24 h of incubation was determined by the colorimetric method described by Sudsai et al [14]. A volume of 10 μ l of MTT solution (5 mg/ml in PBS) was added to each well of 96-well plates and further incubated in a CO 2 incubator for 4 h. The formazan products generated by MTT reduction were dissolved in DMSO.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Anti-HIV-1 Integrase and Anti-Inflammatory Activities of Compounds from Betula alnoides Buch-Ham

Chaniad

Sudsai

Septama

et al. 2019

Advances in Pharmacological Sciences

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Betula alnoides is a medicinal plant in Thai traditional longevity preparations. The crude extracts of this plant possess various biological activities. However, the isolated compounds from this plant have no reports of anti-HIV-1 integrase (IN) activity. Therefore, the present study aims to investigate the anti-HIV-1 integrase and anti-inflammatory effects of isolated compounds from this plant and predict the interaction of compounds with integrase active sites. From the bioassay-guided fractionation of the ethanol extract of B. alnoides stems using chromatographic techniques, five pentacyclic triterpenoid compounds were obtained. They are betulinic acid (1), betulin (2), lupeol (3), oleanolic acid (4), and ursolic acid (5). Compound 2 exhibited the most potent inhibitory activity against HIV-1 IN, with an IC50 value of 17.7 μM. Potential interactions of compounds with IN active sites were investigated using computational docking. The results indicated that active compounds interacted with Asp64, a residue participating in 3′-processing, and Thr66, His67, and Lys159, residues participating in strand-transfer reactions of the integration process. Regarding anti-inflammatory activity, all compounds exerted significant inhibitory effects on LPS-induced nitric oxide production (IC50 < 68.7 μM). Thus, this research provides additional scientific support for the use of B. alnoides in traditional medicine for the treatment of HIV patients.

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Evaluation of the wound healing property of Boesenbergia longiflora rhizomes

Cited by 21 publications

References 17 publications

In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

Anti-inflammatory, wound healing and antioxidant potential of compounds from Dioscorea bulbifera L. bulbils

Evaluation of Anti-HIV-1 Integrase and Anti-Inflammatory Activities of Compounds from Betula alnoides Buch-Ham

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