Evaluation of the wound healing efficacy of new antibacterial polymeric nanofiber based on polyethylene oxide coated with copper nanoparticles and defensin peptide: An in-vitro to in-vivo assessment

Eslaminezhad, Sahba; Moradi, Farhad; Hojjati, Mahmoud Reza

doi:10.1016/j.heliyon.2024.e29542

Cited by 2 publications

(1 citation statement)

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“…Looking at the literature to contextualize our results in a broader scenario, most of the uses of antibacterial materials functionalized with copper imply the synthesis of Cu or Cu oxides' nanoparticles [100,101], and only some of these works reported biocompatibility assays [102,103]. Moreover, a direct comparison with the performances of other materials could be deceitful, considering that different antibacterial methods and related quantifications are adopted [104], i.e., the disk diffusion method [105].…”

Section: In Vitro Assaysmentioning

confidence: 99%

Keratin/Copper Complex Electrospun Nanofibers for Antibacterial Treatments: Property Investigation and In Vitro Response

Tummino,

Cruz-Maya,

Varesano

et al. 2024

Materials

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The frontiers of antibacterial materials in the biomedical field are constantly evolving since infectious diseases are a continuous threat to human health. In this work, waste-wool-derived keratin electrospun nanofibers were blended with copper by an optimized impregnation procedure to fabricate antibacterial membranes with intrinsic biological activity, excellent degradability and good cytocompatibility. The keratin/copper complex electrospun nanofibers were multi-analytically characterized and the main differences in their physical–chemical features were related to the crosslinking effect caused by Cu2+. Indeed, copper ions modified the thermal profiles, improving the thermal stability (evaluated by differential scanning calorimetry and thermogravimetry), and changed the infrared vibrational features (determined by infrared spectroscopy) and the chemical composition (studied by an X-ray energy-dispersive spectroscopy probe and optical emission spectrometry). The copper impregnation process also affected the morphology, leading to partial nanofiber swelling, as evidenced by scanning electron microscopy analyses. Then, the membranes were successfully tested as antibacterial materials against gram-negative bacteria, Escherichia coli. Regarding cytocompatibility, in vitro assays performed with L929 cells showed good levels of cell adhesion and proliferation (XTT assay), and no significant cytotoxic effect, in comparison to bare keratin nanofibers. Given these results, the material described in this work can be suitable for use as antibiotic-free fibers for skin wound dressing or membranes for guided tissue regeneration.

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