Evaluation of the Wound Healing Properties of Luteolin Ointments on Excision and Incision Wound Models in Diabetic and Non-Diabetic Rats

Abstract

Luteolin, which is structurally classified under the flavonoids, is naturally distributed abundant among medicinal plants, vegetables and fruits. The wound healing effects of Luteolin ointments were evaluated for incision and excision wound models on non-diabetic and Streptozotocin induced diabetic rats by using macroscopical, biomechanical, biochemical and histopathological methods. Two different concentrations (0.5 % and 1 % (w/w)) of the Luteolin ointment were prepared with the mixture of glycol stearate:propylene glycol:liquid paraffin (3:6:1) and 0.5 g of the ointments were applied topically on wounds once daily for 7 and 14 days. During the experiments, wounds were visually observed, photographically documented and wound areas were measured. After 7 and 14 days treatment, animals were sacrificed under anesthesia and hydroxyproline measurement and biomechanical analysis were performed. Histopathology of the wound area was evaluated considering features of re-epithelialization, thickness of the granulation tissue, angiogenesis, presence of inflammation, number of mast cells. Outcomes of this study revealed that Luteolin ointments improved wound healing process of skin tissue both in non-diabetic and diabetic wounds. The best wound healing activity was observed in incision and excision wounds (97.6 %, 96.1 %, respectively) treated with 0.5 % (w/w) Luteolin ointment on day 14 according to macroscopic results.

In Vitro Evaluation of Antioxidant, Anti-Inflammatory, Antimicrobial and Wound Healing Potential of Thymus Sipyleus Boiss. Subsp. Rosulans (Borbas) Jalas

“…In silico, in vitro, in vivo studies and a clinical trial with a formulation containing luteolin has shown that luteolin has great therapeutic effect against inflammation-associated disturbances. In addition, the effects of luteolin ointment on the key steps of wound healing, such as cell proliferation, cell migration and collagen deposition, have been proven in previous reports [74,75].…”

Luteolin as a modulator of skin aging and inflammation

“…Similar results were achieved in a wound model using diabetic and non‐diabetic rats. Luteolin treatment resulted in faster wound healing with a higher tensile strength, more collagen, a better epithelial regeneration and less inflammation 60 …”

Antioxidant effect of Hymenaea courbaril L (Jatob) sap on the healing of wounds on mice

“…f. (SK) and Salvia euphratica Montbret, Aucher & Rech. f. var, showing strong wound healing effects with important antimicrobial and antioxidant activities Luteolin (Arachis hypogaea L), naturally distributed among medicinal plants, vegetables and fruits, structurally rich in flavonoids, was tested to evaluate wound healing in diabetic and non-diabetic Wistar rats, through topical applications of the formulation in the wounds, showing that flavonoids increase the formation of collagen fibrils in the tissues of non-diabetic and diabetic wounds, since the phenolic compounds present, known as potent antioxidants, contribute to the process of accelerating healing (Ozay et al, 2018).…”