Evaluation of the Vitotox™ and RadarScreen assays for the rapid assessment of genotoxicity in the early research phase of drug development

Westerink, Walter M.A.; Stevenson, Joe C.R.; Lauwers, Annick; Griffioen, Gerard; Horbach, G. Jean; Schoonen, W.G.E.J.

doi:10.1016/j.mrgentox.2009.04.008

Cited by 68 publications

(45 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Humans do not express an SOS response to mutagenic stimuli as bacteria do. It was nevertheless shown that the VITOTOX test correlates well with, and is often much more sensitive than, other bacterial and mammalian/human mutagenicity tests [Verschaeve et al, 1999;Westerink et al, 2009]. Our results should therefore be considered applicable to humans.…”

supporting

confidence: 49%

See 1 more Smart Citation

Genotoxicity investigation of ELF‐magnetic fields in Salmonella typhimurium with the sensitive SOS‐based VITOTOX test

Verschaeve

Anthonissen

Grudniewska³

et al. 2011

Bioelectromagnetics

View full text Add to dashboard Cite

We performed a genotoxicity investigation of extremely low-frequency (ELF) magnetic fields (MFs, 50 Hz, 100 and 500 µT, 1 and 2 h exposure) alone and in combination with known chemical mutagens using the VITOTOX test. This test is a very sensitive reporter assay of Salmonella typhimurium bacteria based on the SOS response. Our study showed that ELF-MFs do not induce SOS-based mutagenicity in S. typhimurium bacteria and do not show any synergetic effect when combined with chemical mutagens.

show abstract

supporting

confidence: 49%

“…The VITOTOX test can be used as a simple, largely automated, high-throughput genotoxicity test. A detailed description of the test as well as of its merits is given elsewhere [Verschaeve et al, 1999;Muto et al, 2003Muto et al, , 2006Westerink et al, 2009]. In this test, two different S. typhimurium TA104 constructs are used.…”

mentioning

confidence: 99%

Genotoxicity investigation of ELF‐magnetic fields in Salmonella typhimurium with the sensitive SOS‐based VITOTOX test

Verschaeve

Anthonissen

Grudniewska³

et al. 2011

Bioelectromagnetics

View full text Add to dashboard Cite

show abstract

“…The specifi city of the Vitotox TM assay was 94% for Ames test and that of the RadarScreen assay was 74% for clastogenicity. The Vitotox TM was more sensitive (86%) than RadarScreen (77%) assay in comparison with the tested compounds [44].…”

Section: Genetically Engineered Bacteria In Genotoxicologymentioning

confidence: 90%

Environmental Application of Reporter-Genes Based Biosensors for Chemical Contamination Screening

Matejczyk¹,

Rosochacki²

2014

Archives of Environmental Protection

View full text Add to dashboard Cite

Abstract:The paper presents results of research concerning possibilities of applications of reporter-genes based microorganisms, including the selective presentation of defects and advantages of different new scientifi c achievements of methodical solutions in genetic system constructions of biosensing elements for environmental research. The most robust and popular genetic fusion and new trends in reporter genes technology -such as LacZ (β-galactosidase), xylE (catechol 2,3-dioxygenase), gfp (green fl uorescent proteins) and its mutated forms, lux (prokaryotic luciferase), luc (eukaryotic luciferase), phoA (alkaline phosphatase), gusA and gurA (β-glucuronidase), antibiotics and heavy metals resistance are described. Reporter-genes based biosensors with use of genetically modifi ed bacteria and yeast successfully work for genotoxicity, bioavailability and oxidative stress assessment for detection and monitoring of toxic compounds in drinking water and different environmental samples, surface water, soil, sediments.

show abstract

“…This gap has been addressed by assays relying on up-regulation of the RAD54 gene in yeast, including GreenScreen GC, which uses a RAD54-GFP reporter, 5 and RadarScreen, based on a RAD54-luciferase reporter. 3 The higher-throughput assays described above employ nonmammalian cell systems. Hastwell et al 6 developed GreenScreen HC, a reporter gene assay that employs a p53-competent human TK6 lymphoblastoid cell line.…”

Section: Introductionmentioning

confidence: 99%

The BlueScreen-384 Assay as an Indicator of Genotoxic Hazard Potential in Early-Stage Drug Discovery

et al. 2013

View full text Add to dashboard Cite

High-throughput cell-based techniques that permit early detection of compound-induced genotoxic damage have recently become available. Methods based on induction of the GADD45a promoter are attractive because multiple intracellular mechanisms that detect genetic damage intersect at this checkpoint gene. Consequently, assays such as GreenScreen HC, which uses p53-competant human TK6 lymphoblastoid cells and a GADD45a-GFP reporter, have been developed. GreenScreen HC allows weekly testing of dozens of compounds using 96-well microplates, with high interassay consistency. BlueScreen HC is a recent advancement, coupling GADD45a to Gaussia luciferase, with several advantages over GADD45a-GFP including the potential for miniaturization. Here we describe implementation of a 384-well BlueScreen assay. For drug discovery programs carrying out iterative analogue synthesis around a chemical lead series, these assays permit assessment of compound genotoxic potential in parallel to, rather than subsequent to, determination of activity at a therapeutic target. We demonstrate comparability of BlueScreen-384 to GreenScreen HC and illustrate the use of BlueScreen-384 to explore the structure-activity relationship around a genotoxic lead molecule to identify nongenotoxic analogues. BlueScreen-384 can reduce the need for costly and time-consuming analogue testing in more traditional genotoxicity tests, such as the Ames test.

show abstract

Evaluation of the Vitotox™ and RadarScreen assays for the rapid assessment of genotoxicity in the early research phase of drug development

Cited by 68 publications

References 65 publications

Genotoxicity investigation of ELF‐magnetic fields in Salmonella typhimurium with the sensitive SOS‐based VITOTOX test

Genotoxicity investigation of ELF‐magnetic fields in Salmonella typhimurium with the sensitive SOS‐based VITOTOX test

Environmental Application of Reporter-Genes Based Biosensors for Chemical Contamination Screening

The BlueScreen-384 Assay as an Indicator of Genotoxic Hazard Potential in Early-Stage Drug Discovery

Contact Info

Product

Resources

About