Evaluation of the usefulness of molecular markers Xgwm205, Xcfd81, Whs350 for the identification of resistance gene Pm2 to powdery mildew (Blumeria graminis f. sp. tritici) in wheat cultivars of different origins

Tomkowiak, Agnieszka; Kurasiak-Popowska, Danuta; Grynia, Joanna; Mikołajczyk, Sylwia; Weigt, Dorota; Niemann, Janetta; Kiel, Angelika

doi:10.14199/ppp-2017-023

Cited by 2 publications

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“…Tomkowiak et al [38] also evaluated the usefulness of the Xgwm205, Xcfd81 and Whs350 molecular markers to identify the Pm2 resistance gene against powdery mildew of cereals and grasses in 27 wheat varieties. As a result of SSR analyses, the Xgwm205 marker was considered the most effective in Pm2 gene identification; its product appeared in 25 out of 27 analyzed varieties.…”

Section: Discussionmentioning

confidence: 99%

Identification of Powdery Mildew Blumeria graminis f. sp. tritici Resistance Genes in Selected Wheat Varieties and Development of Multiplex PCR

et al. 2019

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The aim of the study was to identify the Pm2, Pm3a, Pm4b and Pm6 genes and to develop multiplex PCR reaction conditions to reduce time and limit analysis costs. The following molecular markers were used for gene identification: Xcfd81, Whs350 and Xgwm205 (for Pm2), Pm3a (for Pm3a), STS_241 and Xgwm382 (for Pm4b), NAU/BCDSTS 135-2 (for Pm6). Plant material consisted of 7 popular European wheat varieties from the wheat collection at the Department of Genetics and Plant Breeding of the Poznań University of Life Sciences. The field experiment was established in 2017 and 2018 on 10 m2 plots in a randomized complete block design in three replicates in the Dłoń Agricultural Experimental Farm of the Poznań University of Life Sciences (51°41’23.835”N 017°4’1.414”E). The analyses demonstrated that the accumulation of all identified Pm genes was found in the Assosan variety. The accumulation of the Pm2, Pm4b and Pm6 genes was found in Atomic, Bussard, Lear, Sparta, Tonacja and Ulka varieties. The work also involved developing multiplex PCR conditions for Xcfd81 and STS_241 and Xcfd81 and Xgwm382 primer pairs, allowing the simultaneous identification of the Pm2 and Pm4b genes.

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Section: Discussionmentioning

confidence: 99%

Identification of Powdery Mildew Blumeria graminis f. sp. tritici Resistance Genes in Selected Wheat Varieties and Development of Multiplex PCR

et al. 2019

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Characteristics of powdery mildew and its importance for wheat grown in Poland

Pietrusińska¹,

Tratwal²

2020

Plant Prot. Sci.

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Powdery mildew of grasses and cereals (Blumeria graminis) is a fungal plant disease which is caused by species of fungi from the Erysiphaceae order. B. graminis is a biotrophic parasite, biologically diverse parasite with a high degree of specialization in certain host species and with numerous physiological breeds adapted to different varieties of a particular host species. In Poland, powdery mildew of cereals and grasses is recorded every year, and its greatest intensity is in south-eastern and south-western regions. The degree of infestation by B. graminis varies every year. This means that the disease occurs every year, in greater or lesser severity. Therefore, it requires monitoring (harmfulness thresholds) and chemical control practically in every vegetation season. Nowadays, an important role is played by immunological breeding. In breeding programs, resistance genes from wild crop forms, primitive and indigenous varieties are an effective tool. The introduction of effective resistance genes into cultivated varieties is a common procedure used in breeding program.<br />The aim of this study was to describe the fungal disease of plants from the group of powdery mildews caused by <br /> B. graminis as an overview.

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Evaluation of the usefulness of molecular markers Xgwm205, Xcfd81, Whs350 for the identification of resistance gene Pm2 to powdery mildew (Blumeria graminis f. sp. tritici) in wheat cultivars of different origins

Cited by 2 publications

References 1 publication

Identification of Powdery Mildew Blumeria graminis f. sp. tritici Resistance Genes in Selected Wheat Varieties and Development of Multiplex PCR

Identification of Powdery Mildew Blumeria graminis f. sp. tritici Resistance Genes in Selected Wheat Varieties and Development of Multiplex PCR

Characteristics of powdery mildew and its importance for wheat grown in Poland

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