Evaluation of the use of conductimetry for the rapid and precise measurement of Salmonella spp. growth rates

Sherry, Aileen; Patterson, Margaret F.; Kilpatrick, D. J.; Madden, Robert H.

doi:10.1016/j.mimet.2006.03.004

Cited by 14 publications

(14 citation statements)

References 7 publications

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“…Juneja and Marks (2006) found that after heat‐injured salmonellas had undergone repair, i.e. completed lag phase, their growth rates were the same as for unstressed cells and Sherry et al. (2006) determined that the isolates used in this study had similar growth rates.…”

Section: Discussionmentioning

confidence: 68%

“…In addition, a sample of the stressed culture was inoculated into TSBYE and loaded into the RABIT to determine the TTD. The detection threshold was c. 1·4 × 10 8 CFU ml −1 (Sherry et al. 2006) under the conditions used.…”

Section: Resultsmentioning

confidence: 98%

“…Indirect impediometry was used to measure the time taken for the 40 serovars to recover and grow to a defined level of activity following the application of the stresses. The method was as detailed by Sherry et al. (2006).…”

Section: Methodsmentioning

confidence: 99%

“…1997). Further, the growth rates of isolates can be rapidly determined using indirect conductimetry (Sherry et al. 2006), and this parameter is important in considering the potential of isolates to reach hazardous levels postprocessing.…”

Section: Introductionmentioning

confidence: 99%

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Use of conductimetry to rapidly determine relative stress sensitivity inSalmonellaisolates

Sherry

Patterson

Madden

2009

Journal of Applied Microbiology

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Aims: To compare conventional plate counting and indirect conductimetry as techniques for ranking the resistance of Salmonella spp. to processing stressors. Methods and Results: Forty Salmonella isolates were subjected to three separate stressors used in food processing; irradiation, heat and high hydrostatic pressure (HHP). Total viable counts (TVC) using conventional plate counts and time to detection (TTD) using indirect conductimetry were determined. A significant negative correlation between TVC and TTD was seen with irradiation (P < 0·01) and heat (P < 0·05) but not HHP. Conclusions: For a group of salmonellas, indirect conductimetry can rapidly determine a ranking of isolate sensitivity to irradiation and heat. However, for HHP, the results indicated that conventional plate counting alone cannot be used to determine sensitivity. Significance and Impact of the Study: The resistance of micro‐organisms to processing systems must be ranked to allow the selection of appropriate isolates for process validation. TTD measurements allow rapid screening of salmonellas to rank isolates for resistance to irradiation and heat stress. However, following HHP, the TVC of survivors is independent of the time required for growth to a set cell density and therefore it cannot be used as the sole measure of relative stress resistance.

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Section: Discussionmentioning

confidence: 68%

Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Use of conductimetry to rapidly determine relative stress sensitivity inSalmonellaisolates

Sherry

Patterson

Madden

2009

Journal of Applied Microbiology

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“…Impedance based methods have included the Malthus, Bactometer, Rabit and Bactrac (Russell, 2000;Duran & Marshall, 2002;Sherry, Patterson, Kilpatrick, & Madden, 2006), and optical methods -VITEK (Odumeru et al, 1999) and MicroFoss (Odumeru & Belvedere, 2002). However, some tests, especially those requiring additional instrumentation, have been hampered by their complexity, high costs, limited applicability to and/or interferences by complex food samples.…”

Section: Introductionmentioning

confidence: 99%