Evaluation of the use of laparoscopic-guided cholecystocholangiography and liver biopsy in definitive diagnosis of neonatal cholestatic jaundice

Abstract

Background:Once it is established that a jaundiced infant has direct hyperbilirubinemia, the principal diagnostic concern is to differentiate hepatocellular from obstructive cholestasis. Traditional tests such as ultrasonography, percutaneous liver biopsy and technetium 99 m hepatobiliary iminodiacetic acid (HIDA) scan are often not sufficiently discriminating. Definitive exclusion of biliary atresia (BA) in the infant with cholestatic jaundice usually requires mini-laparotomy and intra-operative cholangiography. This approach has many drawbacks because those sick infants are subjected to a time-consuming procedure with the probability of negative surgical exploration.Aim of the Study:The aim of this study was to determine the feasibility of laparoscopic-guided cholecystocholangiography (LGCC) and its accuracy and safety in the diagnosis of BA and thus preventing unnecessary laparotomy in infants whose cholestasis is caused by diseases other than BA.Patients and Methods:Twelve cholestatic infants with direct hyperbilirubinemia subjected to LGCC (age, 7–98 days; mean, 56 days) after ultrasound scan and (99 mTc) HIDA scan and percutaneous liver biopsy failed to provide the definitive diagnosis.Results:One patient had completely absent gall bladder (GB) so the laparoscopic procedure was terminated and laparotomy was done (Kasai operation). Four patients had small size GB; they underwent LGCC that showed patent common bile duct with atresia of common hepatic duct, so laparotomy and Kasai operation was performed. Seven patients had well-developed GB, LGCC revealed patent biliary tree, so laparoscopic liver biopsies were taken for histopathology. Five of those patients had neonatal hepatitis, and two had cholestasis as a complication of prolonged TPN. No perioperative complications or mortalities were recorded.Conclusion:When the diagnosis neonatal cholestasis remains elusive after traditional investigations, LGCC is an accurate and simple method for differentiating BA from hepatocellular causes.

The Accuracy of Liver Biopsy to Diagnose Biliary Atresia: A Meta-Analysis

“…Liver biopsy is an invasive examination. There are many complications [29][30][31]. It reported that 5.8%-13.5% infants appear transient bacteremia after liver biopsy.…”

ANKRD1 and SPP1 as diagnostic markers and correlated with immune infiltration in biliary atresia

“…The current gold standard for the diagnosis of BA is exploratory laparotomy or intraoperative laparoscopic cholangiography, but this is an invasive examination method for children. [ 3 ] Abdominal ultrasound and hepatobiliary scintigraphy may be helpful in differentiating BA from other forms of IC. However, the accuracy of abdominal ultrasound is limited due to the experience of the operator.…”

“…Differentiation between BA and other forms of intrahepatic cholestasis (IC) is difficult. The current gold standard for the diagnosis of BA is exploratory laparotomy or intraoperative laparoscopic cholangiography, but this is an invasive examination method for children [3] . Abdominal ultrasound and hepatobiliary scintigraphy may be helpful in differentiating BA from other forms of IC.…”

Hepatic Subcapsular Flow as a Significant Diagnostic Marker for Biliary Atresia: A Meta-Analysis

“…However, patients may occasionally lack the symbolic signature for the early diagnosis of BA; thus, many of them may miss the optimal timing of surgical treatment. Intraoperative cholangiography and liver biopsy remain the gold standard for diagnosing BA, but they are invasive examination methods for children [14][15][16]. Although some markers and examinations have been proved distinct and have the potential for diagnosis of BA, an individual, ideal method has not yet been acknowledged.…”