Evaluation of the use of C-terminal part of the Schistosoma mansoni 200kDa tegumental protein in schistosomiasis diagnosis and vaccine formulation

Carvalho, Gardênia Braz Figueiredo de; Pacífico, Lucila Gonçalves Grossi; Pimenta, Deborah Laranjeira Ferreira; Siqueira, Liliane Maria Vidal; Teixeira-Carvalho, Andréa; Coelho, Paulo Marcos Zech; Pinheiro, Carina S.; Fujiwara, Ricardo Toshio; Oliveira, Sérgio C.; Fonseca, Cristina Toscano

doi:10.1016/j.exppara.2014.02.003

Cited by 21 publications

(14 citation statements)

References 40 publications

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“…It has also been shown that IgG4 reactivity to SEA in ELISA can be used as an immune biomarker for the monitoring of infection with S. mansoni in areas of endemicity (140). Recently, the S. mansoni 200-kDa tegumental protein (Sm200) was used in an ELISA for the diagnosis of S. mansoni infection in a murine model, exhibiting more than 90% sensitivity and specificity, denoting it as a promising reagent for diagnosis (141).…”

Section: Antibody Detectionmentioning

confidence: 99%

Advances in the Diagnosis of Human Schistosomiasis

et al. 2015

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SUMMARY Schistosomiasis is a major neglected tropical disease that afflicts more than 240 million people, including many children and young adults, in the tropics and subtropics. The disease is characterized by chronic infections with significant residual morbidity and is of considerable public health importance, with substantial socioeconomic impacts on impoverished communities. Morbidity reduction and eventual elimination through integrated intervention measures are the focuses of current schistosomiasis control programs. Precise diagnosis of schistosome infections, in both mammalian and snail intermediate hosts, will play a pivotal role in achieving these goals. Nevertheless, despite extensive efforts over several decades, the search for sensitive and specific diagnostics for schistosomiasis is ongoing. Here we review the area, paying attention to earlier approaches but emphasizing recent developments in the search for new diagnostics for schistosomiasis with practical applications in the research laboratory, the clinic, and the field. Careful and rigorous validation of these assays and their cost-effectiveness will be needed, however, prior to their adoption in support of policy decisions for national public health programs aimed at the control and elimination of schistosomiasis.

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Section: Antibody Detectionmentioning

confidence: 99%

Advances in the Diagnosis of Human Schistosomiasis

et al. 2015

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“…The recombinant CCA showed 100% sensitivity and 96% specificity by IgG detection in chronic individuals using magnetic microspheres [79]. The IgG-ELISA using recombinant 200-kDa tegumental protein demonstrated 90% sensitivity and 93.3% specificity with a strong correlation with egg burden in the same set of individuals [75]. El Aswad et al (2011) showed sensitivity and specificity of 89.7% and 100%, respectively, using the recombinant calreticulin and cercarial transformation fluid in ELISA [80].…”

Section: Discussionmentioning

confidence: 99%

Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity infections in endemic area in Brazil Antigen target in schistosomiasis diagnosis

Moraes¹,

Shollenberger

Castro-Borges

et al. 2018

Preprint

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52Background: Despite decades of employment of control programs, schistosomiasis 53 remains a global public health problem. To further reduce prevalence and intensity of 54 infection, or to achieve the goal of elimination in low-endemic areas, there need to be 55 better diagnostic tools to detect low intensity infections in low-endemic areas as Brazil. 56The rationale for development of new diagnostic tools is because in low-endemic 57 settings, the standard Kato-Katz diagnostic method loses its sensitivity and misses low 58 intensity infections. To develop new diagnostic tools, we employed a proteomics 59 approach search for biomarkers associated with schistosome-specific immune responses 60 to develop sensitive and specific new methods for immunodiagnosis. 61 62 Methods and findings: Immunoproteomic analysis was performed on an egg extract of 63 Schistosoma mansoni using pooled sera from infected or non-infected individuals from 64 a low-endemic area of Brazil. Cross reactivity with other helminth parasites was 65 determined using pooled sera from individuals infected with different parasitic 66helminths. Using this approach we identified 23 spots recognized by schistosome acute 67 and chronic sera samples. To identify immunoreactive spots that are likely glycan 68 epitopes, we compared immunoreactivity of spots treated by sodium metaperiodate 69 oxidation of egg extract. This treatment yielded 12/23 spots maintaining 70 immunoreactivity, suggesting they are protein epitopes. From these 12 spots, 11 spots 71 cross-reacted with sera from infection with other helminths and 10 spots cross-reacted 72 with the negative control group. Spot number 5 was exclusively immunoreactive with 73 sera from schistosome-infected groups in native and deglycosylated conditions and 74 corresponds to a Major Egg Antigen. We expressed the Major egg antigen as a 75 recombinant protein and showed by western blot a similar recognition pattern of that of 4 76 the native protein. IgG-ELISA gave a sensitivity of 87.10% and specificity of 89.09% 77 represented by area under ROC curve of 0.95. IgG-ELISA performed better than the 78 conventional K-K (2 slides) identifying 56/64 cases harboring 1-10 egg per gram of 79 feces that were undiagnosed by K-K parasitological technique.80 81 Conclusions: The serological proteome approach was able to identify a new diagnostic 82 candidate. The recombinant egg antigen provided good performance in IgG-ELISA to 83 detect individuals with extreme low-intensity infections (1 egg per gram of feces). 84 Therefore, the IgG-ELISA using this newly identified recombinant major egg antigen 85 can be a useful tool to be combined with other techniques in low-endemic areas to 86 determine the true prevalence of schistosome infection that is underestimated by Kato-87 Katz method. Further, to overcome the complexity of ELISA in the field, a second-88 generation of antibody-based rapid diagnostic tests (RDT) can be developed. 89 90 Recombinant Protein, Endemic Areas. 92 93 94 95 96 97 98 99 5 100 Author Summary 101 102 ...

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“… 70 , 81 , 82 Given these limitations, antibody-based detection methods are not currently the first choice for endemic areas; however, the determination of immunoreactivity against different schistosome antigens can identify infections with loads as low as 1 EPG. 24 , 68 , 83 , 84 , 85 , 86 This fact had led to the introduction of these methods as a complementary tool during epidemiological surveys in low-endemic areas. Its use decreases false-negative cases seen when few K-K slides are applied in conditions of low egg excretion.…”

Section: Antibody Detection Is a Valuable Tool To Identify Egg-negatimentioning

confidence: 99%

Diagnosis of Schistosoma mansoni infections: what are the choices in Brazilian low-endemic areas?

Silva-Moraes

Shollenberger

Siqueira

et al. 2019

Mem. Inst. Oswaldo Cruz

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The population of Brazil is currently characterised by many individuals harbouring low-intensity Schistosoma mansoni infections. The Kato-Katz technique is the diagnostic method recommended by the World Health Organization (WHO) to assess these infections, but this method is not sensitive enough in the context of low egg excretion. In this regard, potential alternatives are being employed to overcome the limits of the Kato-Katz technique. In the present review, we evaluated the performance of parasitological and immunological approaches adopted in Brazilian areas. Currently, the diagnostic choices involve a combination of strategies, including the utilisation of antibody methods to screen individuals and then subsequent confirmation of positive cases by intensive parasitological investigations.

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Evaluation of the use of C-terminal part of the Schistosoma mansoni 200kDa tegumental protein in schistosomiasis diagnosis and vaccine formulation

Cited by 21 publications

References 40 publications

Advances in the Diagnosis of Human Schistosomiasis

Advances in the Diagnosis of Human Schistosomiasis

Serological proteomic screening and evaluation of a recombinant egg antigen for the diagnosis of low-intensity infections in endemic area in Brazil Antigen target in schistosomiasis diagnosis

Diagnosis of Schistosoma mansoni infections: what are the choices in Brazilian low-endemic areas?

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