Evaluation of the transcriptional immune biomarkers in peripheral blood from Warao indigenous associate with the infection by Mycobacterium tuberculosis

Araujo, Zaida; Palacios, Alberto; Enciso-Moreno, Leonor; López-Ramos, Juan Ernesto; Wide, Albina; Waard, Jacobus H. de; Rivas-Santiago, Bruno; Serrano, Carmen; Bastián-Hernández, Y.; Castañeda-Delgado, Julio Enrique; Enciso-Moreno, José Antonio

doi:10.1590/0037-8682-0516-2018

Cited by 3 publications

(6 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Receiver Operating Characteristic (ROC) curves analysis, Student's t-test, Fisher's exact test, Mann Whitney test, Kruskal-Wallis and Dunn's multiple comparison tests were performed as previously reported [15,16]. A scattergram was plotted using GraphPad Prism software version 5.02 (Trial version, GraphPad-Sofware, Inc., San Diego, USA).…”

Section: Discussionmentioning

confidence: 99%

“…Peripheral blood samples from both groups were drawn for QuantiFERON-TB Gold In-Tube (QFT-IT) testing (Commercial test QuantiFERON-TB Gold In-Tube, Cellestis, Victoria, Australia). Inclusion criteria took into account the recommendation previously reported [16].…”

Section: Study Populationmentioning

confidence: 99%

“…Individuals who had evidence of clinical symptoms suggesting pulmonary TB infection were excluded after having been diagnosed as having pulmonary TB using at least one of the following previously applied criteria published [16,[26][27][28]. Individuals who had been prescribed immunosuppressive drugs (i.e., corticosteroids, azathioprine, and cyclophosphamide) were also excluded, as were participants who did not sign an informed consent agreement.…”

Section: Clinical Features Microscopy the Tuberculin Skin Test And Ch...mentioning

confidence: 99%

“…Blood samples under non-stimulation conditions and stimulation conditions with M. tuberculosis antigens, Early Secretory Antigenic Target-6 (ESAT-6), Culture Filtrate Protein-10 (CFP10), and TB antigen 7.7 (TB7.7) were studied. A real-time reverse transcription polymerase chain reaction (RT-qPCR) assay was performed as previously reported [16].…”

Section: Isolated Blood Cells and Gene Biomarker Amplificationsmentioning

confidence: 99%

“…Recently, we identified host biomarkers associated with LTBI in M. tuberculosis antigen-stimulated whole blood assays and IgG antibody serum from a TB endemic population in Delta's Orinoco. A host biomarker signature of IgG anti-P-12037, MMP-9, CCR5 and CCL11 was identified; high IgG anti-P-12037 and MMP-9 and low CCR5 and CCL11 levels, relative to controls, were associated with LTBI [15,16].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Immune Biomarker Combinations for Diagnosis Monitoring of Latent Tuberculosis Infection

Zaida¹,

Ernesto²,

Antonio³

et al. 2023

jdismarkers

View full text Add to dashboard Cite

Objective: Global Tuberculosis (TB) eradication efforts must also focus on detecting and treating cases of Latent TB Infection (LTBI); persons with LTBI can progress to active TB at any time, often many years or even decades after the initial infection, thereby serving as a source of new infections. Methods: The aim was evaluated the diagnostic accuracy of the combination of serological host biomarkers that may support the differentiation between LTBI and Non-Infected (NI) individuals. A total of 182 adult Warao Amerindians were included; cases with LTBI (n=103) and Non-Infected (NI) individuals (n=79). The Real-time quantitative PCR (qPCR) was performed on all peripheral blood samples from Warao Amerindians and analyzed transcriptional immune biomarkers (i.e., IFN-γ, CD14, MMP-9, CCR5, CCL11, CXCL9/MIG, and uPAR/PLAUR proteins) under stimulation condition with ESAT-6, CFP10, and TB7.7 Mycobacterium Tuberculosis (Mtb)-antigens. Additionally, Enzyme-Linked Immunosorbent Assays (ELISA) were performed for evaluating host biomarker anti-synthetic peptides (5 ESAT-6 and 17 Ag85A synthetic peptides) covering Mtb antigen sequences. Results: The approach’s diagnostic information was compared using Receiver Operating Characteristic (ROC) curves. The ROC analysis revealed high biosignature discriminative ability for the relative gene expression of MMP-9 high levels (AUC=0.799 ± 0.071: 0.640 - 0.917, 95% CI), p < 0.002) between LTBI and NI; additionally IgG anti-synthetic peptide; ESAT-6 P-12037 (AUC=0.640; 0.545-0.735 95% CI, p<0.007) allowed differentiation between LTBI and NI or healthy ones. Conclusion: The accuracy of the MMP-9/IgG anti-P-12037 combination could have a high discriminative ability for diagnosing LTBI; such an approach holds promise for further validation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Study Populationmentioning

confidence: 99%

Section: Clinical Features Microscopy the Tuberculin Skin Test And Ch...mentioning

confidence: 99%

Section: Isolated Blood Cells and Gene Biomarker Amplificationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Immune Biomarker Combinations for Diagnosis Monitoring of Latent Tuberculosis Infection

Zaida¹,

Ernesto²,

Antonio³

et al. 2023

jdismarkers

View full text Add to dashboard Cite

show abstract

Diagnostic Potential of the Serological Response to Synthetic Peptides from Mycobacterium tuberculosis Antigens for Discrimination Between Active and Latent Tuberculosis Infections

Araujo

Waard

Camargo

et al. 2022

Int J Pept Res Ther

View full text Add to dashboard Cite

Screening of potential biomarkers for distinguishing between latent and active tuberculosis in children using bioinformatics analysis

et al. 2021

View full text Add to dashboard Cite

Tuberculosis (TB) is one of the leading causes of childhood morbidity and death globally. Lack of rapid, effective non-sputum diagnosis and prediction methods for TB in children are some of the challenges currently faced. In recent years, blood transcriptional profiling has provided a fresh perspective on the diagnosis and predicting the progression of tuberculosis. Meanwhile, combined with bioinformatics analysis can help to identify the differentially expressed genes (DEGs) and functional pathways involved in the different clinical stages of TB. Therefore, this study investigated potential diagnostic markers for use in distinguishing between latent tuberculosis infection (LTBI) and active TB using children's blood transcriptome data. From the Gene Expression Omnibus database, we downloaded two gene expression profile datasets (GSE39939 and GSE39940) of whole blood-derived RNA sequencing samples, reflecting transcriptional signatures between latent and active tuberculosis in children. GEO2R tool was used to screen for DEGs in LTBI and active TB in children. Database for Annotation, Visualization and Integrated Discovery tools were used to perform Gene Ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway analysis. STRING and Cytoscape analyzed the protein-protein interaction network and the top 15 hub genes respectively. Receiver operating characteristics curve was used to estimate the diagnostic value of the hub genes. A total of 265 DEGs were identified, including 79 upregulated and 186 downregulated DEGs. Further, 15 core genes were picked and enrichment analysis revealed that they were highly correlated with neutrophil activation and degranulation, neutrophil-mediated immunity and in defense response. Among them TLR2, FPR2, MMP9, MPO, CEACAM8, ELANE, FCGR1A, SELP, ARG1, GNG10, HP, LCN2, LTF, ADCY3 had significant discriminatory power between LTBI and active TB, with area under the curves of 0.84, 0.84, 0.84, 0.80, 0.87, 0.78, 0.88, 0.84, 0.86, 0.82, 0.85, 0.85, 0.79, and 0.88 respectively. Our research provided several genes with high potential to be candidate gene markers for developing non-sputum diagnostic tools for childhood Tuberculosis.

show abstract

Evaluation of the transcriptional immune biomarkers in peripheral blood from Warao indigenous associate with the infection by Mycobacterium tuberculosis

Cited by 3 publications

References 25 publications

Immune Biomarker Combinations for Diagnosis Monitoring of Latent Tuberculosis Infection

Immune Biomarker Combinations for Diagnosis Monitoring of Latent Tuberculosis Infection

Diagnostic Potential of the Serological Response to Synthetic Peptides from Mycobacterium tuberculosis Antigens for Discrimination Between Active and Latent Tuberculosis Infections

Screening of potential biomarkers for distinguishing between latent and active tuberculosis in children using bioinformatics analysis

Contact Info

Product

Resources

About