Evaluation of the toxicity effects of silk fibroin on human lymphocytes and monocytes

Naserzadeh, Parvaneh; Mortazavi, Seyed Alireza; Ashtari, Khadijeh; Salimi, Ahmad; Farokhi, Mehdi; Pourahmad, Jalal

doi:10.1002/jbt.22056

Cited by 13 publications

(7 citation statements)

References 39 publications

(50 reference statements)

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“…In this line, some authors reported a limited inflammatory effect after 24 h of incubation with silk microparticles of 45–125 µm at 5 mg/mL [ 35 ]. Basophils, eosinophils, monocytes, and lymphocytes did not experience changes in their levels with respect to the control group, in agreement with previous studies where toxicity in lymphocytes and monocytes due to oxidative stress was studied at concentrations higher than 50 µg/mL of SFN [ 36 ].…”

Section: Discussionsupporting

confidence: 91%

In Vitro Hemocompatibility and Genotoxicity Evaluation of Dual-Labeled [99mTc]Tc-FITC-Silk Fibroin Nanoparticles for Biomedical Applications

et al. 2023

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Nuclear imaging is a highly sensitive and noninvasive imaging technique that has become essential for medical diagnosis. The use of radiolabeled nanomaterials capable of acting as imaging probes has shown rapid development in recent years as a powerful, highly sensitive, and noninvasive tool. In addition, quantitative single-photon emission computed tomography (SPECT) images performed by incorporating radioisotopes into nanoparticles (NPs) might improve the evaluation and the validation of potential clinical treatments. In this work, we present a direct method for [99mTc]Tc-radiolabeling of FITC-tagged silk fibroin nanoparticles (SFN). NPs were characterized by means of dynamic light scattering and scanning electron microscopy. In vitro studies were carried out, including the evaluation of stability in biological media and the evaluation of hemocompatibility and genotoxicity using the cytokinesis block micronucleus (CBMN) assay. The radiolabeling method was reproducible and robust with high radiolabeling efficiency (∼95%) and high stability in biological media. Hydrodynamic properties of the radiolabeled NPs remain stable after dual labeling. The interaction of SFN with blood elicits a mild host response, as expected. Furthermore, CBMN assay did not show genotoxicity induced by [99mTc]Tc-FITC-SFN under the described conditions. In conclusion, a feasible and robust dual-labeling method has been developed whose applicability has been demonstrated in vitro, showing its value for further investigations of silk fibroin NPs biodistribution in vivo.

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Section: Discussionsupporting

confidence: 91%

In Vitro Hemocompatibility and Genotoxicity Evaluation of Dual-Labeled [99mTc]Tc-FITC-Silk Fibroin Nanoparticles for Biomedical Applications

et al. 2023

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“…For instance, the stiffness of a scaffold is responsible for the formation of a brindled structure for skeletal myoblasts, stimulation of capillary tubes for endothelial cells, and neurite outgrowth for neuron cells [14]. Since cellular fate is modulated by cell-scaffold interactions, efforts have been done to regulate cellular responses by controlling the topography, 3D geometry, or chemical composition of cell substrates [15,16]. Additionally, some external factors can potentially affect cell–material interactions and biocompatibility including: Physical stimulation using surface topology; biochemical stimulations using release of growth factors; and mechanical and electrical stimulation (ES) [17,18,19].…”

Section: Introductionmentioning

confidence: 99%

Electrically Conductive Materials: Opportunities and Challenges in Tissue Engineering

et al. 2019

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Tissue engineering endeavors to regenerate tissues and organs through appropriate cellular and molecular interactions at biological interfaces. To this aim, bio-mimicking scaffolds have been designed and practiced to regenerate and repair dysfunctional tissues by modifying cellular activity. Cellular activity and intracellular signaling are performances given to a tissue as a result of the function of elaborated electrically conductive materials. In some cases, conductive materials have exhibited antibacterial properties; moreover, such materials can be utilized for on-demand drug release. Various types of materials ranging from polymers to ceramics and metals have been utilized as parts of conductive tissue engineering scaffolds, having conductivity assortments from a range of semi-conductive to conductive. The cellular and molecular activity can also be affected by the microstructure; therefore, the fabrication methods should be evaluated along with an appropriate selection of conductive materials. This review aims to address the research progress toward the use of electrically conductive materials for the modulation of cellular response at the material-tissue interface for tissue engineering applications.

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“…An interesting study conducted by Naserzadeh et al [ 79 ] revealed the toxic effect of SF. Application of silk nanoparticles on isolated fibroblasts and human umbilical vein endothelial cells (HUVECs) indicated mitochondrial dysfunction, reactive oxygen species (ROS) production, and lipid peroxidation, connected with cytochrome c release.…”

Section: Molecular Mechanisms Of Sf Actionmentioning

confidence: 99%

Silk Fibroin Biomaterials and Their Beneficial Role in Skin Wound Healing

et al. 2022

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The skin, acting as the outer protection of the human body, is most vulnerable to injury. Wound healing can often be impaired, leading to chronic, hard-to-heal wounds. For this reason, searching for the most effective dressings that can significantly enhance the wound healing process is necessary. In this regard, silk fibroin, a protein derived from silk fibres that has excellent properties, is noteworthy. Silk fibroin is highly biocompatible and biodegradable. It can easily make various dressings, which can be loaded with additional substances to improve healing. Dressings based on silk fibroin have anti-inflammatory, pro-angiogenic properties and significantly accelerate skin wound healing, even compared to commercially available wound dressings. Animal studies confirm the beneficial influence of silk fibroin in wound healing. Clinical research focusing on fibroin dressings is also promising. These properties make silk fibroin a remarkable natural material for creating innovative, simple, and effective dressings for skin wound healing. In this review, we summarise the application of silk fibroin biomaterials as wound dressings in full-thickness, burn, and diabetic wounds in preclinical and clinical settings.

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Evaluation of the toxicity effects of silk fibroin on human lymphocytes and monocytes

Cited by 13 publications

References 39 publications

In Vitro Hemocompatibility and Genotoxicity Evaluation of Dual-Labeled [99mTc]Tc-FITC-Silk Fibroin Nanoparticles for Biomedical Applications

In Vitro Hemocompatibility and Genotoxicity Evaluation of Dual-Labeled [99mTc]Tc-FITC-Silk Fibroin Nanoparticles for Biomedical Applications

Electrically Conductive Materials: Opportunities and Challenges in Tissue Engineering

Silk Fibroin Biomaterials and Their Beneficial Role in Skin Wound Healing

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